Cosmosil Protein-R columns - Hplc.eu

Wide Pore HPLC Column for Protein ChemistryCOSMOSIL Protein-R・Excellent Separation・High recovery rate・Outstanding Stability at Low pHSilica GelAverage Particle SizeAverage Pore SizeStationary PhaseMain InteractionHigh Purity Porous Spherical Silica5 μmapprox. 300ÅOctadecyl GroupHydrophobic InteractionSiOHOctadecyl GroupCOSMOSIL Protein-R is a reversed phase HPLC column designed specificallyfor protein and peptide separation. COSMOSIL Protein-R provides significantlyimproved peak shapes, high recovery rate and outstanding stabilityat low pH, which are often problematic for the separation of proteins andpeptides with conventional C18-300Å and C4-300Å columns.Improved SeparationCOSMOSIL Protein-R shows sharper peaks than conventional C18 wide pore columns.COSMOSIL Protein-RC18-300(A company)C4-300(B company)Column SizeMobile PhaseFlow RateTemperatureDetection4.6 mm I.D. x 150 mmA: 0.05% TFA - 20% AcetonitrileB: 0.05% TFA - 60% AcetonitrileB conc. 0 100% 20 min Linear Gradient1.0 ml/min30UV 220nmSample1. Ribonuclease A2. Cytochrome C3. Lysozyme4. BSA5. Myoglobin6. Ovalbumin(1.0 μg each)(MW: 13700)(MW: 12400)(MW: 14300)(MW: 66000)(MW: 17000)(MW: 45000)Recovery RateCOSMOSIL Protein-R shows a higher recovery rate thanconventional C18-300Å and C4-300Å columns.DurabilityCOSMOSIL Protein-R provides more consistent retentiontime and longer column lifetimes than conventional C4-300Å .Protein-RC18-300(A company)C4-300(B company)Myoglobin (M.W. 17000)BSA(M.W. 66000)Cytochrome C (M.W. 12400)Ribonuclease A (M.W. 13700)120100 80604020 COSMOSIL Protein-R C4-300(B company)0% 20% 40% 60% 80% 100%0050 100time (hr)C4-300ÅC18-300ÅProblemsWeak Acid ResistanceLow Separation CapacityLow Recovery RateAdvantages of COSMOSIL Protein-RCOSMOSIL Protein-R solves these problems, and providesdramatic improvement of separation capasity and recoveryrate with high acid resistance.

Preparative Separation4.6 mm I.D. x 250 mm 4.6 mm I.D. x 250 mm10.0 mm I.D. x 250 mmTotal Protein 6 μg (1 μg each) Total Protein 600 μg (100 μg each) Total Protein 3000 μg (500 μg each)Sample1. Ribonuclease A (MW: 13700)2. Cytochrome C (MW: 12400)3. Lysozyme (MW: 14300)4. BSA5. Myoglobin6. Ovalbumin(MW: 66000)(MW: 17000)(MW: 45000)Application Data・Peptides・High Molecular Weight ProteinsColumn SizeMobile PhaseFlow RateTemperatureDetectionSample4.6 mm I.D. x 150 mmA: 0.05% TFA / WaterB: 0.05% TFA / AcetonitrileB conc. 10 40% 20 min Linear Gradient1.0 ml/min30 o CUV 220 nm1. Oxytocin2. Angiotensin II (Human)3. Angiotensin I (Human)4. Substance P(0.8 μg each)Column SizeMobile PhaseFlow RateTemperatureDetectionSample4.6 mm I.D. x 150 mmA: 0.05% TFA / 20%AcetonitrileB: 0.05% TFA / 60%AcetonitrileB conc. 0 100% 10 min Linear Gradient1.0 ml/min30 o CUV 220 nm, 0.256AUFS1. Fibrinogen (4.0 μg)2. Catalase (2.0 μg)3. Thyroglobulin (6.0 μg)・Peptides5C18-MS-II (pore size 120) shows better separation of low molecular weight proteins, but COSMOSIL Protein-R shows better separation of high molecular weight proteins.Column SizeMobile PhaseFlow RateTemperatureDetectionSample4.6 mm I.D. x 150 mmA: 0.05% TFA / WaterB: 0.05% TFA / AcetonitrileB conc. 0 35% 60 min Linear Gradient1.0 ml/min30 o CUV 220 nmPoly-L-Glutamic Acid Sodium Salt(MW: 1500-5000) (200 μg)5C18-MS-IIProtein-ROrdering InformationProduct Name Column Size Product NumberCOSMOSIL Protein-R 2.0 mm I.D. x 150 mm 06514-71Packed Column 4.6 mm I.D. x 50 mm 06525-314.6 mm I.D. x 150 mm 06526-214.6 mm I.D. x 250 mm 06527-1110.0 mm I.D. x 150 mm 06529-9110.0 mm I.D. x 250 mm 06530-5120.0 mm I.D. x 150 mm 06531-4120.0 mm I.D. x 250 mm 06532-31Other size may be available. Please enquire.Product Name Column Size Product NumberCOSMOSIL Protein-R 4.6 mm I.D. x 10 mm 06518-31Guard Column 10.0 mm I.D. x 20 mm 06528-0120.0 mm I.D. x 20 mm 08692-81

COSMOSIL C18-AR-300, C8-AR-300, C4-AR-300, Ph-AR-300COSMOSIL offers a variety of stationary phases with wide-pore silica gel material for separations of polypeptides and proteins.Stationary Phase5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300SiOHSiOHSiOHSiOHOctadecyl Group Octyl Group Butyl Group Phenyl GroupCarbon Content approx. 12% approx. 7% approx. 6% approx. 7%USP Category L1 L7 L26 L11Main InteractionHydrophobic InteractionHydrophobic Interactionπ-π InteractionPacking MaterialHigh Purity Porous Spherical SilicaAverage Particle Size5 μmAverage Pore Sizeapprox. 300 ÅComparison of Separation5C18-AR-3005C8-AR-300 5C4-AR-300 5Ph-AR-300Column SizeMobile PhaseFlow RateTemperatureDetection4.6 mm I.D. x 150 mmA; 0.05%TFA - 20%AcetonitrileB; 0.05%TFA - 60%AcetonitrileB conc. 0 100% 20min Linear Gradient1.0 ml/min30UV 220 nmSampleHemoglobin, Bovine (10 μg)For more data on C18-AR-300, C8-AR-300, C4-AR-300, Ph-AR-300, please visit our website at www.nacalai.comCOSMOSIL ApplicationCOSMOSIL Application, which includes more than 7,000 applications using COSMOSIL columns, is available on our webiste.The application is searchable by sample category, sample name, CAS No., column name and particle size. For more information,please visit www.nacalai.com