CURRICULUM VITAE

CV and publications Norbert Müller 1
CURRICULUM VITAE
Name Norbert Müller
Date of birth October 2, 1956
Place of birth Constance, Germany
Nationality German
Professional training/career
2003 - present Associated Professor at the Institute of Parasitology, University of Berne, Switzerland;
Head of the Human Medical Parasitology Unit, Supervisor of various applied and ba-
sic research projects (see funding and publication list)
1993-2003 “Assistent” and “Oberassistent” (since 1995) at the Institute of Parasitology, University
of Berne, Switzerland
1993 Habilitation at the Phil. nat. Faculty, University of Berne: Alveolar echinococcosis and
African trypanosomiasis: molecular biological approaches in immunodiagnosis and
vaccine development
1992 Research fellow at the Dept. of Animal Health and Biomedical Sciences (Prof. Dr. J.
Mansfield), University of Wisconsin, Madison, USA
1986-1992 Postdoc at the Institute of General Microbiology, University of Berne: Research on
immunological and molecular mechanisms involved in host-parasite interaction (Afri-
can trypanosomiasis, echinococcosis); Development of serological and molecular
tools for diagnosis of parasitic infections
1981-1986 PhD in molecular microbiology, University of Constance, Germany (Supervisor: Prof.
Dr. W. Boos): Sugar transport in Salmonella typhimurium
1976-1981 Diploma in biology, University of Constance, Germany (Supervisor: Prof. Dr. W.
Funded projects (since 2007)
boos): Focus on molecular biology and microbiology
Research grant for “Antiparasitic activity and mode of action of thiazolides“, COST-B22 project from
the SBF (2006-2007) (principal investigator: N. Müller, co-investigator. PD Dr. J. Müller, Institute of
Parasitology, Berne)

CV and publications Norbert Müller 2
Research grant, for "Genomic approaches to the selection of new antigens for serological diagnosis of
helminth infections“, from Rudolf Geigy Foundation (2006-2007) (principal investigator: PD Dr. S. Fel-
ger; Swiss Tropical and Public Health Institute, Basle, co-investigator: N. Müller)
Research grant, for "Genomic approaches to the selection of new antigens for serological diagnosis of
helminth infections“, from Roche Foundation Foundation (since 2007) (principal investigator: PD Dr. S.
Felger; Swiss Tropical and Public Health Institute, Basle, co-investigator: N. Müller)
Research grant, for „Genomic approaches to the selection of new antigens for serological diagnosis of
helminth infections“, from Velux Foundation (2007-2008) (principle investigators: N. Müller, PD Dr. S.
Felger; Swiss Tropical and Public Health Institute, Basle)
Research grant, for "Molecular and biochemical characterization of the cellular functions involved in
drug restance formation in Giardia lamblia“, from Swiss National Science Foundation (2007-2010)
(principle investigator: N. Müller, co-investigator: PD Dr. J. Müller, Institute of Parasitology, Berne)
Research grant, for "Untersuchung des Lebensraums von freilebenden pathogenen Amöben [FLA]
(Naegleria fowleri und andere), from Federal Office for Civil Protection (Spiez Laboratory) (2008-2010)
(principle investigators: N. Müller, Prof. B. Gottstein, Institute of Parasitology, Berne)
Research grant, for "Tritrichomonas foetus bei der Katze: Was bedeutet das für das Rind?" from BVET
(2010-2011) (principle investigator: Dr. C. Frey, Institute of Parasitology, Berne; co-investigators: N.
Müller, Prof. Dr. B. Gottstein, Institute of Parasitology, Berne)
Research grant: Analysis of pathogenicity mechanisms associated with Naegleria fowleri infections
from Federal Office for Civil Protection (Spiez Laboratory) (since 2011) (principle investigators: N.
Müller, Prof. B. Gottstein, Institute of Parasitology, Berne)
Research grant: Functional analysis of nitroreductases from the intestinal protozoan parasite Giardia
lamblia. from Swiss National Science Foundation (since 2012) (principle investigator: N. Müller, co-
investigator: PD Dr. J. Müller, Institute of Parasitology, Berne)
Further activities
More than 115 publications in peer-reviewed scientific journals, editorial board member of a scientific
journal (Parasitology International), reviewer for more than 30 scientific journals (e.g. Nature, Science,
Journal of Immunology), reviewer for national and international grant applications, member of the ex-
ecutive committee of the Swiss Society of Tropical Medicine and Parasitology (SGTP), member of the
Swiss Society of Biochemistry (SGB).

CV and publications Norbert Müller 3
List of publications (1982 – present).
1. Peer-reviewed original articles
Müller. N., Heine, H.G, Boos, W. (1982) Cloning of mglB, the structural gene of the galactose-binding
protein of Salmonella typhimurium and Escherichia coli. Molecular and General Genetics 185, 473-
480.
Benner, D., Müller. N. and Boos, W. (1985) Temperature-sensitive catabolite activator protein in Escherichia
coli BUG6. Journal of Bacteriology 161, 347-352.
Müller. N. Heine, H.G. and Boos, W. (1985) Characterization of the Salmonella typhimurium mgl operon
and its gene products. Journal of Bacteriology 163, 37-45.
Vogel, M., Gottstein, B., Müller. N., Seebeck, T. (1988) Production of a recombinant antigen of Echinococcus
multilocularis with high immunodiagnostic sensitivity and specificity. Molecular and Biochemical
Parasitology 31, 117-126.
Müller. N., Gottstein, B., Vogel, M., Flury, K., Seebeck, T. (1989a) Application of a recombinant Echinococcus
multilocularis antigen in an enzymelinked immunosorbent assay for immunodiagnosis of
human alveolar echinococcosis. Molecular and Biochemical Parasitology 36, 151-160.
Müller. N., Gottstein, B., Vogel, M., Scholle, A., Seebeck, T. (1989b) Plasmid vector for overproduction
and export of recombinant protein in Escherichia coli: efficient one-step purification of a recombinant
antigen from Echinococcus multilocularis (Cestoda). Gene 75, 329-332.
Gottstein, B., Müller. N., Cryz, S., Vogel, M., Tanner, I. and Seebeck, T. (1990) Humoral and cellular
immune response in mice and dogs induced by a recombinant Echinococcus multilocularis antigen
produced by a Salmonella typhimurium vaccine strain. Parasite Immunology 12, 163-174.
Müller. N., Hemphill, A., Imboden, M., Duvallet, G., Dwinger, R.H., Seebeck, T. (1992) Identification
and characterization of two repetitive non-variable antigens from African trypanosomes which are recognized
early during infection. Parasitology 104, 111-120.
Müller. N., Imboden, M., Detmer, E., Seebeck, T. (1993) Cytoskeleton-associated antigens from African
trypanosomes are recognized by self-reactive antibodies of uninfected mice. Parasitology 107,
411-417.
Vogel, M., Müller. N., Gottstein, B., Flury, K., Eckert, J., Seebeck. T. (1991) Echinococcus multilocularis:
Characterization of a DNA probe. Acta Tropica 48, 109-116.
Troll, H., Winckler, T., Lascu, I., Müller. N., Saurin, W., Veron, M., Mutzel, R. (1993) Mitochondrial
nucleoside diphosphate kinase from Dictyostelium discoideum defines an organelle branch of the enzyme
family. The Journal of Biological Chemistry 268, 25469-25475.
Affolter, M., Hemphill, A., Roditi, I., Müller. N., Seebeck, T. (1994) The repetitive microtubuleassociated
proteins MARP1 and MARP2 of Trypanosoma brucei. Journal of Structural Biology 112,
241-251.
Imboden, M., Müller. N., Hemphill, A., Mattioli, R., Seebeck, T. (1995) Repetitive proteins from the
flagellar cytoskeleton of African trypanosomes are diagnostically useful antigens. Parasitology 110,
249-258.
Felleisen, R., Zimmermann, V., Gottstein, B., Müller. N. (1996) Use of a 96 well format for the affinity
purification of maltose binding protein (MBP) fusion proteins. BioTechniques 20, 618-620.
Hemphill, A., Stäger, S., Gottstein, B., Müller. N. (1996) Electron microscopical investigation of surface
alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody. Parasitology
Research 82, 206-210.

CV and publications Norbert Müller 4
Müller. N., Stäger, S., Gottstein, B. (1996) Serological analysis of antigenic heterogeneity of Giardia
lamblia variant surface proteins. Infection and Immunity 64, 1385-1390.
Müller. N., Zimmermann, V., Hentrich, B., Gottstein, B. (1996) Diagnosis of Neospora caninum and
Toxoplasma gondii by PCR and DNA-Hybridization Immunoassay. Journal of Clinical Microbiology 34,
2850-2862.
Müller. N., Mansfield, J.M., Seebeck, T. (1996) Trypanosome variant surface glycoproteins are recognized
by self-reactive antibodies in uninfected hosts. Infection and Immunity 64, 4593-4597.
Weber, K. Schneider, A., Müller. N., Plessmann, U. (1996) Polyglycylation of tubuline in the diplomonad
Giardia lamblia, one of the oldest eukaryotes. FEBS Letters 393, 27-30.
Detmer, E., Hemphill, A., Müller. N., Seebeck, T. (1997) The Trypanosoma brucei autoantigen I/6 is an
internally repetitive cytoskeletal protein. European Journal of Cell Biology 72, 378-384.
Stäger, S. , Felleisen, R., Gottstein, B., Müller. N. (1997) Giardia lamblia variant surface protein (VSP)
H7 stimulates a heterogeneous repertoire of antibodies displaying differential cytological effects on the
parasite. Molecular and Biochemical Parasitology 85, 113-124.
Stäger, S. Gottstein, B., Müller. N. (1997b) Systemic and local antibody response in mice induced by a
recombinant peptide fragment from Giardia lamblia variant surface protein (VSP) H7 produced by a
Salmonella typhimurium vaccine strain. International Journal for Parasitology 27, 965-971.
Stäger, S., Müller. N. (1997) Giardia lamblia infections in B-cell-deficient transgenic mice. Infection and
Immunity 65, 3944-3946.
Weber, K., Schneider, A., Westermann, S., Müller. N., Plessmann, U. (1997) Posttranslational modifications
of α- and β-tubulin in Giardia lamblia. FEBS Letters 419, 87-91.
Hemphill, A., Felleisen, R., Connolly, B., Gottstein, B., Hentrich, B. and Müller. N. (1997) Characterization
of a cDNA-clone encoding Nc-p43, a major Neospora caninum tachyzoite surface protein. Parasitology
115, 581-590.
Felleisen, R., Lambelet, N., Bachmann, P., Nicollet, J., Müller. N. and Gottstein, B. (1998) Detection of
Tritrichomonas foetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences.
Journal of Clinical Microbiology 36, 513-519.
Stäger, S., Gottstein, B., Sager, H. Jungi, T.W., Müller. N. (1998) Influence of antibodies in mother's
milk on antigenic variation of Giardia lamblia in the murine mother-offspring model of infection. Infection
and Immunity 66, 1287-1292.
Gottstein, B., Hentrich, B., Wyss, R., Thür B., Busato, A., Stärk, K.D.C., Müller. N. (1998) Molecular
and immunodiagnostic investigations on bovine neosporosis in Switzerland. International Journal for
Parasitology 28, 679-691.
Müller N., Stäger S. (1999) Periodic appearance of a predominant variant antigen-type during a
chronic Giardia lamblia infection in a mouse model International Journal for Parasitology 29, 1917-
1923.
Gottstein, B. Hentrich, B., Wyss, R., Thür, B., Müller. N., Kaufmann, H., Waldvogel, A. (1999) Molekular-
und immundiagnostische Untersuchungen zur bovinen Neosporose in der Schweiz. Schweizer
Archiv für Tierheilkunde 141, 59-68.
Kern, B. Maurer, C.A., Schilling, M., Müller. N., Büchler, M.W. (2000) Ein seltener Fall einer disseminierten
intra- und retroperitonealen Infektion mit Echinococcus cysticus. Viszeralchirurgie 35, 275-278.
Wyss, R., Sager, H., Müller. N., Inderbitzin, F., König, M., Audigé, L., Gottstein, B. (2000) Untersuchungen
zum Vorkommen von Toxoplasma gondii und Neospora caninum unter fleischhygienischen
Aspekten. Schweizerisches Archiv für Tierheilkunde 142, 95-108.

CV and publications Norbert Müller 5
Bienz, M. Wittwer, P., Zimmermann, V., Müller. N. (2001) Molecular characterization of a predominant
antigenic portion of Giardia lamblia variant surface protein H7. International Journal for Parasitology
31, 827-832.
Müller. N., Sager, H., Hemphill, A., Mehlhorn, H. , Heydorn, A. O., Gottstein, B. (2001) Comparative
molecular investigation of Nc5-PCR amplicons from Neospora caninum NC-1 and Hammondia heydorni-Berlin-1996.
Parasitology Research 87, 883-885.
Bienz, M., Siles-Lucas, M., Wittwer, P., Müller. N. (2001). vsp gene expression by Giardia lamblia
clone GS/M-83-H7 during antigenic variation in vivo and in vitro. Infection and Immunity 69, 5278-
5285.
Bienz, M., Siles-Lucas, M., Müller. N. (2001) Use of a novel DNA melting profile assay for the identification
of PCR-amplified genomic sequences encoding for variant-specific surface proteins (VSPs)
from the clonal GS/M-83-H7 line of Giardia lamblia. Parasitology Research 87, 1011-1015.
Müller. N., Vonlaufen, N., Gianinazzi, C., Leib, S., Hemphill, A. (2002). Application of real-time fluorescent
PCR for quantitative assessment of Neospora caninum infections in organotypic slice cultures of
rat central nervous system. Journal of Clinical Microbiology 40, 252-255.
Vonlaufen, N., Gianinazzi, C., Müller. N., Simon, F., Björkman, C., Jungi, T.W., Leib, S., A. Hemphill.
(2002) Infection of organotypic slicecultures from rat central nervous tissue with Neospora caninum:
An alternative approach to study host-parasite interactions. International Journal for Parasitology 32,
533-542.
Gottstein, B., Dai, W.J., Walker, M., Stettler, M., Müller. N., Hemphill, A. (2002) An intact laminated
layer is important for the establishment of secondary Echinococcus multilocularis infection, Parasitology
Research, 88, 822-828.
Vonlaufen, N., Müller. N., Keller, N., Naguleswaran, A., Bohne, W., McAllister, M.M:., Björkman, C.,
Müller, E., Caldelari, R., Hemphill, A. (2002) Exogenous nitric oxide triggers Neospora caninum
tachyzoite-to-bradyzoite stage conversion in murine epidermal keratinocyte cell cultures. International
Journal for Parasitology 32, 1253-1265.
Bienz, M., Dai, W.J., Welle, M., Gottstein, B., Müller. N. (2003) Interleukin-6-deficient mice are highly
susceptible to Giardia lamblia infections but exhibit normal intestinal IgA responses against the parasite.
Infection and Immunity, 71, 1569-1573.
Müller. N., Vonlaufen, N., Gianinazzi, C., Leib, S., Hemphill, A. (2002) Application of real-time fluorescent
PCR for quantitative assessment of Neospora caninum infections in organotypic slice cultures of
rat central nervous system. Journal of Clinical Microbiology 40, 252-255.
Vonlaufen, N., Gianinazzi, C., Müller. N., Simon, F., Björkman, C., Jungi, T.W., Leib, S., A. Hemphill.
(2002) Infection of organotypic slice cultures from rat central nervous tissue with Neospora caninum:
An alternative approach to study host-parasite interactions. International Journal for Parasitology 32,
533-542.
Gottstein, B., Dai, W.J., Walker, M., Stettler, M., Müller. N., Hemphill, A. (2002) An intact laminated
layer is important for the establishment of secondary Echinococcus multilocularis infection. Parasitology
Research 88, 822-828.
Vonlaufen, N., Müller. N., Keller, N., Naguleswaran, A., Bohne, W., McAllister, M.M:, Björkman, C.,
Müller, E., Caldelari, R., Hemphill, A. (2002) Exogenous nitric oxide triggers Neospora caninum
tachyzoite-to-bradyzoite stage conversion in murine epidermal keratinocyte cell cultures. International
Journal for Parasitology 32, 1253-1265.
Cannas, A., Naguleswaran, A., Müller. N., Eperon, S., Gottstein, B., Hemphill, A. (2003) Vaccination of
mice against experimental Neospora caninum infections using NcSAG1- and NcSRS2-based recombinant
antigens and DNA vaccines. Parasitology 126, 303-312.

CV and publications Norbert Müller 6
Cannas, A., Naguleswaran, A., Müller. N., Gottstein, B., Hemphill, A. (2003) Reduced cerebral infection
of Neospora caninum-infected mice after vaccination with recombinant microneme protein
NcMIC3 and ribi adjuvant. Journal of Parasitology 89, 44-50.
Marti, M., Regos, A., Li, Y., Schraner, E.M., Wild, P., Müller. N., Knopf, L.G., Hehl, A.B. (2003) An
ancestral secretory apparatus in the protozoan parasite Giardia intestinalis. Journal of Biological
Chemistry 278, 24837-24848.
Müller. N., Zimmermann, V., Forster, U., Bienz, M., Gottstein, B.,Welle, M. (2003) PCR-based detection
of canine Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration
of a protocol for quality assessment of the diagnostic amplification reaction. Veterinary Parasitology
114, 223–229.
Naguleswaran, A., Müller. N., Hemphill A. (2003) Neospora caninum and Toxoplasma gondii: a novel
adhesion/invasion assay reveals distinct differences in tachyzoite-host cell interactions. Experimental
Parasitology 104, 149-158.
Bienz, M., Dai, W.J., Welle, M., Gottstein, B., Müller. N. (2003) Interleukin-6-deficient mice are highly
susceptible to Giardia lamblia infections but exhibit normal intestinal IgA responses against the parasite.
Infection and Immunity 71, 1569-1573.
Althaus, H., Müller. N., Busato, A., Mellor, P.S., Torsteinsdottir, S., Marti, E. (2004) Cloning and sequencing
of a cDNA expressing a ribosomal P0 peptide from Culicoides nubeculosus (Diptera). Veterinary
Immunology and Immunopatholology 99, 99-111.
Nunes, C.P., Zaha, A., Gottstein, B., Müller. N., Siles-Lucas, M. (2004) 14-3-3 gene characterization
and description of a second 14-3-3 isoform in both Echinococcus granulosus and E. multilocularis.
Parasitology Research 93, 403-409.
Kurdova, R., Müller. N., Tsvetkova, N., Michov, L., Georgieva, D., Ivanova, M., and Gottstein, B.
(2004) Characterisation of Trichinella isolates from Bulgaria by molecular typing and cross-breeding.
Veterinary Parasitololgy 123, 179-188.
Vonlaufen, N., Guetg, N., Naguleswaran, A., Müller. N., Bjorkman, C., Schares, G., von Blumröder, D.,
Ellis, J., and Hemphill, A. (2004) In vitro induction of Neospora caninum bradyzoites in vero cells reveals
differential antigen expression, localization, and host-cell recognition of tachyzoites and
bradyzoites. Infection and Immunity 72, 576-583.
Ammann, P., Waldvogel, A., Breyer, I., Esposito, M., Müller. N., Gottstein, B. (2004) The role of B- and
T-cell immunity in toltrazuril-treated C57BL/6 WT, micro MT and nude mice experimentally infected
with Neospora caninum. Parasitology Research 94, 178-187.
Tsvetkova, N., Schild, M., Panaiotiv, S., Kurdova-Mintcheva, R., Gottstein, B., Walochnik, J., Aspöck,
H., Siles Lucas, M., Müller. N. (2004) Taxonomic identification of free-living amoebae isolated from the
environment in Bulgaria. Parasitology Research 92, 405-413.
von Allmen, N., Bienz, M., Hemphill, A., Müller. N. (2004) Experimental infections of neonatal mice
with cysts of Giardia lamblia clone GS/M-83-H7 are associated with an antigenic reset of the parasite.
Infection and Immunity 72, 4763-4771.
van Maanen, C. , Wouda, W., Schares, G., von Blumröder; D., Conraths, F. J., Norton, D., Williams, J.
L., Esteban-Redondo, I., Innes, E. A. , Mattsson, J. G., Björkman, C., Fernández-García, A., Ortega-
Mora, L. M., Müller. N., Sager, H. Hemphill, A. (2004) An interlaboratory comparison of Immunohistochemistry
and PCR methods for detection of Neospora caninum in bovine foetal tissues. Veterinary
Parasitology 126, 351-364.
Gottstein, B., Razmi, G. R. , Ammann, P., Sager, H., Müller. N. (2005) Toltrazuril treatment to control
diaplacental Neospora caninum transmission in experimentally infected pregnant mice . Parasitology
130, 41-48.
Rüfenacht, S., Sager, H., Müller. N., Schaerer, V., Heier, A., Welle, M.M., and Roosje, P. J. (2005)
Two cases of feline Leishmania infections in Switzerland. Veterinary Record 156, 542.

CV and publications Norbert Müller 7
Brachelente, C., Müller. N., Doherr, M.G., Sattler, U., Welle, M. (2005) Cutaneous leishmaniosis in
naturally infected dogs is associated with a Th2-biased immune response. Veterinary Pathology 42,
166-175.
von Allmen, N., Bienz, M., Hemphill, A., Müller. N. (2005) Quantitative assessment of sense and antisense
transcripts from genes involved in antigenic variation (vsp genes) and encystation (cwp 1
gene) of Giardia lamblia clone GS/M-83-H7. Parasitology, 130, 389-396.
Scheidegger, A., Vonlaufen, N., Naguleswaran, A., Gianinazzi, C., Müller. N., Leib, S., and Hemphill,
A. (2005) Differential effects of IFN-gamma and TNF-alpha on Toxoplasma gondii proliferation in organotypic
rat brain slice cultures. Journal of Parasitology, 91, 307-315
B Esposito, M., Stettler, R., Moores, S. L., Pidathala, C., Müller. N., Stachulski, A., Berry, N. G., Rossignol,
J. F., Hemphill, A. (2005) In vitro efficacies of nitazoxanide and other thiazolides against Neospora
caninum tachyzoites reveal antiparasitic activity independent of the nitro group. Antimicrobial
Agents and Chemotherapy, 49, 3715-3723
Gianinazzi, C., Schild, M., Müller. N., Leib, S. L., Simon, F., Nunez, S. Joss, P., Gottstein, B. (2005)
Organotypic slice cultures from rat brain tissue. A new approach for Naegleria fowleri CNS infection in
vitro. Parasitology, 131, 797-804
Rehmann, P., Gröne, A., Gottstein, B., Sager, H., Müller. N., Vollm, J., Bacciarini, L. N. (2005) Alveolar
echinococcosis in the zoological garden Basle. Schweizerisches Archiv für Tierheilkunde 147, 498-
502.
Müller, J., Rühle, G., Müller. N., Rossignol, J. F., Hemphill, A. (2006) In vitro effects of thiazolides on
Giardia lamblia WB C6 cultured axenically and in co-culture with Caco2 cells. Antimicrobial Agents
and Chemotherapy, 50, 162-170.
Matsumoto, J., Müller. N., Hemphill, A., Oku, Y., Kamiya M., Gottstein, B. (2006) 14-3-3- and II/3-10-
gene expression as molecular markers to address viability and growth activity of Echinococcus metacestodes.
Parasitology, 132, 83-94.
Sager, H., Moret, C.S., Müller. N., Staubli, D., Esposito, M., Schares, G., Hässig, M., Stärk, K., Gottstein,
B. (2006) Incidence of Neospora caninum and other intestinal protozoan parasites in populations
of Swiss dogs. Veterinary Parasitology, 139, 84-94.
von Allmen, N., Christen, S., Forster, U., Gottstein, B., Welle, M., Müller. N. (2006) Acute trichinellosis
increases susceptibility to Giardia lamblia infection in the mouse model. Parasitology, 133, 139-149.
Haag, K. L., Gottstein, B., Müller. N., Schnorr, A. Ayala, F. J. (2006) Redundancy and recombination in
the Echinococcus AgB multifamily: is there any similarity with protozoan contingency genes? Parasitology,
133, 411-419.
Esposito, M., Müller. N., Hemphill, A. (2007) Structure-activity relationships from in vitro efficacies of
the thiazolide series against the intracellular apicomplexan protozoan Neospora caninum. International
Journal for Parasitology, 37, 183-190.
Graichen, D.A., Gottstein, B., Matsumoto, J., Müller. N., Zanotto, P.M., Ayala, F.J., Haag, K.L. (2007)
Expression and diversity of Echinococcus multilocularis AgB genes in secondary infected mice: evaluating
the influence of T-cell immune selection on antigenic variation. Gene, 392, 98-105.
Cortes, H.C.E., Müller. N., Esposito, M., Leitao, A., Naglueswaran, A., Hemphill. A. (2007) In vitro
efficacy of nitro- and bromo-thiazolyl-salicylamide compounds (thiazolides) against Besnoitia besnoiti
infection in Vero cells. Parasitology, 134, 975-985.
Schild, M., Gianinazzi, C., Gottstein, B., Müller. N. (2007) PCR-based detection of Naegleria sp. infection
in formalin-fixed and paraffin-embedded brain sections. Journal of Clinical Microbiology, 45, 564-
567.

CV and publications Norbert Müller 8
Müller. N., Frei, E., Nunez, S., Gottstein, B. (2007) In vitro-generated Echinococcus multilocularis antigen
to improve serodiagnosis of alveolar echinococcosis by ELISA and immunoblotting. Parasitology,
134, 879-888.
Cortes, H.C.E., Reis, Y., Gottstein, B., Hemphill, A., Leitao, A., Müller. N. (2007) Application of conventional
and real-time fluorescent ITS1 rDNA PCR for detection of Besnoitia besnoiti infections in
bovine skin biopsies. Veterinary Parasitology, 146, 352-356.
Müller, J., Wastling, J., Sanderson, S., Müller. N., Hemphill, A. (2007) A novel Giardia lamblia nitroreductase,
GINR1, interacts with nitazoxanide and other thiazolides. Antimicrobial Agents and
Chemotherapy, 51, 1979-1986.
Müller, J., Sterk, M., Hemphill, A., Müller. N. (2007) Characterization of Giardia lamblia WB C6 clones
resistant to nitazoxanide and to metronidazole. Journal of Antimicrobial Chemotherapy, 60, 280-287.
Sterk, M., Müller, J., Hemphill, A., Müller. N. (2007) Characterisation of a Giardia lamblia WB C6 clone
resistant to the isoflavone formononetin, Microbiology, 153, 4150-4158.
Müller, J., Naguleswaran, A., Müller. N., Hemphill, A. (2008) Functional inhibition of Neospora caninum
protein disulfide isomerase by the 5-nitrothiazole-analogue nitazoxanide and other thiazolides. Experimental
Parasitology, 118, 80-88.
Ben Nouir, N., Nunez, S., Frei, E, Gorcci, M., Müller. N., Gianinazzi, C., Nouri, A., Mekki, M., Babba,
H., Gottstein, B. (2008) Post-surgical follow-up (by ELISA and immunoblotting) of cured versus no
cured cystic echinococcosis in young patients. Parasitology, 135, 105-114.
Ben Nouir, N., Nunez, S., Gianinazzi, C., Müller. N., Nouri, A, Babba, H., and Gottstein, B. (2008) Assessment
of Echinococcus granulosus somatic antigens for serological follow-up of young patients
surgically treated for cystic echinococcosis. Journal of Clinical Microbiology 46, 1631-1640.
Müller, J., Ley, S. Felger, I., Hemphill, A., Müller. N. (2008) Identification of differentially expressed
genes in a Giardia lamblia WB C6 clone resistant to nitazoxanide. Journal of Antimicobial Chemotherapy
62, 72-82.
Ryhner, T., Müller, N., Balmer, V., Gerber, V. (2008) Increased mucus accumulation in horses chronically
affected with recurrent airway obstruction is not associated with up-regulation of CLCA1, EGFR,
MUC5AC, Bcl-2, IL-13 and INF-gamma expression. Veterinary Immunology and Immunopathology,125:
8-17.
Strohbusch, M., Müller. N., Hemphill. A., Greif, G., Gottstein, B. (2008) NcGRA2 as a molecular target
to assess the parasiticidal activity of toltrazuril against Neospora caninum. Parasitology, 135:1065-
1073.
Ben Nouir, N., Gianinazzi, C., Gorcii, M., Müller. N., Nouri, A., Babba, H., Gottstein, B. (2009) Isolation
and molecular characterization of recombinant Echinococcus granulosus P29 protein (recP29) and its
assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients.
Transactions of the Royal Society of Tropical Medicine and Hygiene, 103: 355-364.
Frey, C.F., Schild, M., Hemphill, A., Stünzi, P., Müller. N., Gottstein, B., Burgener, I.A. (2009) Intestinal
Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR. Parasitology
Research, 104, 783-788.
Gianinazzi, C., Schild, M., Wüthrich, F., Ben Nouir. N., Füchslin, H.P., Schürch, N., Gottstein, B.,
Müller. N. (2009) Screening Swiss water bodies for potentially pathogenic free-living amoebae. Research
in Microbiology, 160, 367-374.
Frey, C.F., Schuppers, M.E., Müller. N., Ryser-Degiorgis, M.P., Gottstein, B. (2009) Assessment of the
prevalence of Trichinella spp. in red foxes and Eurasian lynxes from Switzerland. Veterinary Parasitology,
159 :295-299 (2009).

CV and publications Norbert Müller 9
Gianinazzi, C., Schild, M., Wüthrich, F., Müller. N., Schürch, N., Gottstein, B. (2009) Potentially human
pathogenic Acanthamoeba isolated from a heated indoor swimming pool in Switzerland. Exerimenta
Parasitology, 121, 180-186.
Gianinazzi, C., Schild, M., Zumkehr, B., Wüthrich, F., Nüesch, I., Ryter, R., Schürch, N., Gottstein, B.,
Müller. N. (2009) Screening of Swiss hot spring resorts for potentially pathogenic free-living amoebae.
Exerimental Parasitology, 2009 Dec 29. [Epub ahead of print]
Müller. N., Welle, M., Lobsiger, L., Stoffel, M.H., Kühni Boghenbor, K., Hilbe, M., Gottstein, B., Frey,
C.F., Geyer, C., von Bomhard, W. (2009) Occurence of Leishmania sp. in cutaneous lesions of horses
in Cenral Europe. Veterinary Parasitology, 166, 346-351.
Strohbusch, M., Müller. N., Hemphill, A., Krebber, R., Greif, G., Gottstein, B. (2009) Toltrazuril treatment
of congenitally acquired Neospora caninum infection in newborn mice. Parasitology Research,
104, 1335-1343.
Strohbusch, M., Müller. N., Hemphill, A., Margos, M., Grandgirard, D., Leib, S., Greif, G., Gottstein, B.
(2009) Neospora caninum and bome marrow-derived dendritic cells: parasite survival, proliferation and
induction of cytokinenexpression. Parasite Immunology, 31, 366-372.
Müller, J., Nillius, D., Hehl, A., Hemphill, A., Müller. N. (2009) Stable expression of Escherichia coli
beta-glucuronidase A (GusA) in Giardia lamblia: application to high-throughput drug susceptibility testing.
Journal of Antimicrobial Chemotherapy, 64, 1187-1191
Buonomano, R., Brinkmann, F., Leupin, N., Boscacci, R., Zimmermann, A., Müller, N., Fux, C.A.
(2009) Holiday souvenirs from the Mediterranean: Three instructive cases of visceral leishmaniasis.
Scandinavian Journal of Infectious Diseases, 41, 777-781
Gottstein, B., Wittwer, M., Schild, M., Merli, M., Leib, S.L., Müller, N., Müller, J., Jaggi, R. (2010) Hepatic
gene expression profile in mice perorally infected with Echinococcus multilocularis eggs. PLoS
One, 5,e9779.
List, C., Qi, W., Maag, E., Gottstein. B., Müller, N., Felger, I. (2010) Serodiagnosis of Echinococcus
spp. infection: explorative selection of diagnostic antigens by peptide microarray. PLoS Neglegted
Tropical Diseases, 4, e771.
Mejri, N., Müller, N., Hemphill, A., Gottstein, B. (2011) Intraperitoneal Echinococcus multilocularis infection
in mice modulates peritoneal CD4+ and CD8+ regulatory T cell development. Parasitology
International, 60, 45-53.
Gianinazzi C, Schild M, Zumkehr B, Wüthrich F, Nüesch I, Ryter R, Schürch N, Gottstein B, Müller N.
(2010) Screening of Swiss hot spring resorts for potentially pathogenic free-living amoebae. Experimental
Parasitology,126, 45-53.
Stadelmann, B., Spiliotis, M., Müller, J., Scholl, S., Müller, N., Gottstein, B., Hemphill, A. (2010) Echinococcus
multilocularis phosphoglucose isomerase (EmPGI): a glycolytic enzyme involved in metacestode
growth and parasite-host cell interactions. International Journal for Parasitology, 40, 1563-
1574.
Berger-Schoch, A.E., Herrmann, D.C., Schares, G., Müller, N., Bernet, D., Gottstein, B., Frey, C.F.
(2010) Prevalence and genotypes of Toxoplasma gondii in feline faeces (oocysts) and meat from
sheep, cattle and pigs in Switzerland. Veterinary Parasitology, 177, 290-297.
Cortes, H.C., Müller, N., Boykin, D., Stephens, C.E., Hemphill, A. (2011) In vitro effects of arylimidamides
against Besnoitia besnoiti infection in Vero cells. Parasitology, 138, 583-592.
Nillius, D., Müller, J., Müller, N. (2011). Nitroreductase (GlNR1) increases susceptibility of Giardia
lamblia and Escherichia coli to nitro drugs. Journal of Antimicrobial Chemotherapy, 66, 1029-1035.
Schweiger, A., Grimm, F., Tanner, I., Müllhaupt, B., Bertogg, K., Müller, N., Deplazes, P. (2011) Serological
diagnosis of echinococcosis: the diagnostic potential of native antigens. Infection, 40, 139-152.

CV and publications Norbert Müller 10
Barlow, A.M., Gottstein, B., Müller, N. (2011) Echinococcus multilocularis in an imported captive European
beaver (Castor fiber) in Great Britain. Veterinary Record, 169, 339.
Burri, D.C., Gottstein, B., Zumkehr, B., Hemphill, A., Schürch, N., Wittwer, M., Müller, N. (2012) Development
of a high- versus low-pathogenicity model of the free-living amoeba Naegleria fowleri. Microbiology,
in press
2. Review articles
Müller. N., Felleisen, R. (1995) Bacterial expression systems as tools for the production of immunodiagnostic
parasite antigens. Parasitology Today 11, 476-480.
Felleisen, R., Müller. N., Yamage, M., Gottstein, B. (1996) Diagnostische PCR in der Veterinärparasitologie:
Tritrichomonose, Neosporose/Toxoplasmose, Echinokokkose/Zystizerkose. Schweizer Archiv
für Tierheilkunde. 138, 144-151 (invited review).
Müller. N., Gottstein, B. (1998) Antigenic variation and the murine immune response to Giardia lamblia.
International Journal for Parasitology 28, 1829-1839 (invited review).
Hemphill, A., Müller. N., Sager, H., Gottstein B. (2000) Neospora caninum und Neosporose – Grundlagenforschung
am Institut für Parasitologie und mögliche Anwendungen. Schweizer Archiv für Tierheilkunde
142, 257-263 (invited review).
Augustine, P.C., Barta J.R., Innes, L., Müller. N. (2001) Chasing coccidia-new tools enter the race
(meeting report). Trends in Parasitology 17, 509-511.
Welle, M., Brachelente, C., Müller. N. (2005) PCR-based detection and quantification of canine
Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies. Advances in Veterinary
Dermatology, 5, 314-318.
Müller. N., von Allmen, N. (2005) Recent insights into the mucosal reactions associated with Giardia
lamblia infections. International Journal for Parasitology, 35, 1339-1347.
Müller. N., Sager, H., Schuppers, M., Gottstein, B. (2006) Methoden zur Untersuchung von Trichinella
Infektionen bei Haus- und Wildschwein. Schweizer Archiv für Tierheilkunde, 148, 463-471.
Hemphill, A., Müller. N., Müller, J. ( 2007) Structure function relationship of thiazolides, a novel class of
anti-parasitic drugs, investigated in intracellular and extracellular protozoan parasites and larval stage
cestodes. Anti-Infective Agents and Medical Chemistry, 6, 273-282.
Hemphill, A., Stadelmann, B., Scholl, S., Müller, J., Spiliotis, M., Müller, N., Gottstein, B., Siles-Lucas,
M.. (2010) Echinococcus metacestodes as laboratory models for the screening of drugs against cestodes
and trematodes. Parasitology, 137, 569-87.
Frey, C. F., Müller, N. (2012) Tritrichomonas - Systematics of an enigmatic genus. Molecular and Cellular
Probes 26, 132-136.
3. Book chapters
Müller. N., Lucius, R. (2006) Antiparasitäre Impfstoffe. Lehrbuch der Allgemeinen Parasitologie, Enke
Verlag, Stuttgart. Lucius, Gottstein, Hiepe eds. 435-444.
Mattson, J., Müller. N. (2007) Neosporosis: Polymerase Chain Reaction. In: Protozoal abortion in farm
ruminants – Guidelines for diagnosis and control; CAB International, Oxfordshire, UK. Ortega-Mora,
Gottstein, Conraths, Buxton eds. 59-63.
Müller, J. Hemphill, A, Müller, N. (2011) Treatment of giardiasis and drug resistance. In: Giardia - a
model organism. Springer Verlag Wien, Austria