DBSC-001 Operation Manual - Mrclab.com

Safety Warnings and Guidelines1. Important operation information of the security:Before the users’ operation, they should have a perfect conception of how touse the Instrument. Therefore, read this Manual carefully before using it.Operation before reading the Manual is forbidden. Read theguidelines and directions below and carry out the countermeasureaccording to them.2. Security:The operation, maintenance and repair of the Instrument should comply withthe basic guidelines and the remarked warning below. If you don’t comply withthem, it will have effect on the scheduled using life of the Instrument and theprotection provided.This product is a normal and an indoor Instrument.Read the Manual carefully before operation, The expert of wiringof equipment Read Read the the can operate Manual Manual this carefully carefully Instrument.before before operation, operation, The expert The expert of wiring ofequipment can operate this wiring equipment canInstrument.operate this Instrument.The operator should not open or repair the Instrument byhimself, which will result in losing the qualification of repairguarantee or occur accident. If there is some wrong with theInstrument, the company will repair it.A.C. power’s grounding should be reliable to safeguardagainst an electric shock. The 3-pin plug supplied withthermo-shaker’s power cable is a safety device that should bematched with a suitable grounded socket.During the normal operation, the temperature of metal blockwill be very high. There will be scald or boiling of the liquid.Therefore strictly prohibit any part of the body to touch theInstrument from scald.Close the test tube lids before put the test tubes into the block.Liquids maybe spill in the block or onto the device if tube lidsopened, that will damage the block or the device.

Before power on, guarantee the voltage used should beaccordant to the voltage needed, and the rated load of electricaloutlet should not lower than the demand. If the electric line isdamaged, you should replace it with the same type. You shouldassure there’s nothing on the electric line and you should not putthe electric line in the ambulatory place. Hold the jack when youpull out the electric line, and don’t pull the electric line.The Instrument should be put in the place of low temperature,little dust, no water and no sun or strong lamp. What’s more, theplace should be good aeration, no corrosively gas or strongdisturbing magnetic field, far away from central heating, campstove and other hot resource. Don't put the Instrument in wet anddusty place. The vent on the Instrument is designed for aeration.Don’t wall up or cover the vent in order to keep from hightemperature. If you use the more than one Instrument the sametime, the distance between them should be more than 100cm.Mains switch is on the rear of the device, push “I” topower on the device, and push “O” to power off thedevice.Power off when you finish your work. Pull off the connectorplug when there’s long time no use of the Instrument and cover itwith a cloth or plastic paper to prevent from dust.Pull the connector plug from the jack at once in the followingcase, and contact the vendor: There is some liquid flowing into the Instrument; Drenched or fire burned. Abnormal operation: such as abnormal sound or smell. Instrument dropping or outer shell damaged. The function has obviously changed.3. The maintenance of InstrumentThe well in the block should be cleaned by the cloth stained with alcohol toassure good heat translation between the block and the test tube and no pollution.If there are smutches on the Instrument, clean them with cloth.Power off when cleaning the Instrument.When cleaning the well, don’t drop the cleaning liquid in the well.Corrosive cleaning liquid is strongly prohibited.

ContentsChapter 1 Introduction--------------------------------------------------------------1Chapter 2 Specifications-----------------------------------------------------------21 The normal operating condition-------------------------------------------22 The basic parameters and performance--------------------------------2Chapter 3 Preparations-------------------------------------------------------------31 Structure Description----------------------------------------------------------32 Keyboard and Display panel------------------------------------------------43 Key Functions--------------------------------------------------------------------5Chapter 4 Operation Guide--------------------------------------------------------61 Single Temperature, Speed and Time setting-------------------------62 Multi-points Programs Setting---------------------------------------------73 How to Shut Off the Temperature Speed and Timing------------- 84 Short Mix--------------------------------------------------------------------------85 Temperature Calibration-----------------------------------------------------86 Exchange of Block------------------------------------------------------------12Chapter 5 Failure analysis and troubleshooting-------------------------13Annex 1 Wiring Diagram of DBSC-001 Thermo Shaker------------------14

DBSC-001 Thermo Shaker Operations Manualchapter 1 IntroductionChapter 1 IntroductionThe Thermo-shaker for Micro-tubes is an ideal instrument for intensive mixing ofsamples in the regulated temperature conditions.Mixing and heating modes can be used both simultaneously and independently i.e,the device can work as shaker and as a thermostat. The main body of the Mixing Blockcan be used with different kinds of blocks. DBSC-001 is applicable for DNA analysis,extraction of lipids and other cell components, DNA library creation, PCR amplification,pre-denaturation in electrophoresis, serum solidification etc.Features of this product as follows:1. As it is equipped with various optional mixing blocks, the instrument can adapt todifferent tubes & wells to cope with experimental needs. It is easy to replace the metalblocks and is very simple to clean and sterilize. Customized blocks are available tosuit your specific demands2. LCD display. It Easy to setup and use3. Simultaneous display of set and actual time, temperature and speed4. Over heating protection device ensures safety & reliability5. Temperature can be calibrated to meet user's needs6. Beep-signal / Stop after program completion7. Can be produced for OEM customers─ 1 ─

DBSC-001 Thermo Shaker Operations ManualChapter 3 PreparationsChapter 3 PreparationsThis chapter is introduces Thermo Shaker’s mechanical structure, the keyboardand each key’s functions and some preparations before power-on. You should befamiliar with this chapter before the Thermo Shaker is first operated。1. Structure DescriptionStandard BlockKeyboardPower SwitchPower connectorFuse─ 3 ─

DBSC-001 Thermo Shaker Operations ManualChapter 3 Preparations2. Keyboard and Display panelSeg.Temp.(℃) Speed (rpm) Time (h:m)Prog.PVSVLCDShortMixSeg. Temp. Speed TimeStartStopDisplay Panel:Section in runCurrent temperature Current speed Remnant timeS1 100.0 1201 10:30S1 100.0 1200 15:00Setting SectionSetting temperature Setting speed Setting time─ 4 ─

DBSC-001 Thermo Shaker Operations ManualChapter 3 Preparations3. Key FunctionsSeg To select procedure section: five segment points asS1,S2,S3,S4,S5.Temp Temperature setting key. Pressing“”or“”to set runningtemperature , To set temperature though pressing“”or“”continuously, which is more fast and conveniently.Speed Speed setting key. Pressing“”or“”to set mixing speed,Toset mixing speed though pressing “”or“”continuously,which is more fast and conveniently. Each time the key ispressed , the mix speed changes by 10rpm.TimeTiming setting key. Pressing“”or“”to set timing hours,Toset timing hours though pressing “”or“”continuously,which is more fast and conveniently.ProgProgrammable setting key. Pressing “prog” to make section fromS1to S2,or S1 to S2 to S3,or S1 to S2 to S3 to S4, or S1 to S2 toS3 to S4 to S5 running continuously.Short MixThe device mixes at the frequency visible in the display for aslong as the Short Mix key is held down. The time is counted inseconds until 999S has expired.Start/StopStop/start key. Pressing this key to stop or start the procedure.Pressing momentary to start, Pressing continuously to stop.─ 5 ─

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guideChapter 4 Operation Guide1. Single temperature, speed and timing settinga) The LCD will display the picture as the chartwhen the instrument powers on and theinstrument goes into the initial state with thesound of “du…”.System-Testing......b) About 6 seconds,the display window forpractical temperature shows 30.0, which isthe practical temperature of the block. Settingtemperature shows 37.0, which is theestablishment temperature. While 10:00 is theformer timing time, and S1 is the formertemperature section. The temperature unit is“℃”, the speed unit is “RPM” and the timeunit is “hour:minute”.c) Pressing Temp’s “”or“”,the value ofdisplay windows for setting temperature willincrease or reduce from decimal digit, unitdigit, tens digit to hundreds digit.S1 30.0 0 00:00S1 37.0 1000 10:00Temp S1 30.0 0 00:00S1 40.0 1000 10:00MSpeed S1 30.0 0 00:00S1 40.0 1200 10:00MPressing Speed or Time’s “”or“”,to setmix speed or timing time according to thesame transformation rule above.Besides, pressing “”or“” for 2 secondscontinuously to amend the digit from decimalto unit ,from unit to tens digit ,from tens digitto hundreds digit quickly.Time S1 30.0 0 00:00S1 40.0 1200 2:00Start/StopStart single temperature procedure— 6 —

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guideIf you want to set temperature to 40.0℃, mix speed to 1200 rpm ,timing time to 2hour, pressing temp’s “” continuously to let the temperature reach 40.0, it willbe confirmed and stored at once. Press Speed’s “” continuously to let themix speed reach 1200rpm ,Meanwhile pressing time’s “” continuously to letthe timing time reach 02:00, it will be confirmed and stored at once too. Afterfinishing setting section S1, pressing “stop/start” key to run S1 program.Heating begins afterStart/Stop has been pressed or one of the Temp. curor keys has been pressed.d) Pressing seg’s“” “” to select onesection of S1,S2,S3,S4,S5. Then set thevalues of temperature, speed and timeaccording to step (C).You can set these five points as usual segmentpoint, whenever you want to use,you can transferone of them.Seg S2 30.0 0 00:00S2 50.0 1400 3:002. Multi-points programs settinga) Pressing “prog” key , can connect S1-S2, S1-S2-S3, S1-S2-S3-S4, S1-S2-S3-S4-S5into two-points or multi-points program.Note!the first section in the multi-points program is only for S1b) If set S1-S2-S3-S4 multi-points program,pressing“prog” Key , the display window show “Star:S1End:S2”, “Start:S1” means the first section of themulti-points program is S1,it can not amend, “End:S2” means the end section of the multi-pointsprogram is S2, it can amend , then pressing seg’s“”, let S2 reach S4, pressing “prog” to confirm.Then the multi-points progam S1-S2-S3-S4 is settingfinish. Now the setting section shows S14.PROG to validateStar:S1 End:S2Seg ProgS1 30.0 0 00:00S14 50.0 1400 3:00Start/Stop— 7 —Run multi-points program

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guidec) Pressing “stop/start”key to run S1-S2-S3-S4 multi-points program。Note!We also can press “stop/start” key to run the multi-points programafter we have selected the end section.3. How to shut off the temperature, speed and timing functiona) Press seg’s“” “” key to Select one section of the S1,S2,S3,S4,S5.b) Press Temp’s “” key , the lowest value displayes “OFF” , and the deviceremeans at room temperature. Also press Speed or Time’s “” key, the lowestvalue displayes “OFF”, Speed displayes “OFF”, it means no mixing function. Timedisplayes “OFF” , it means no timing function, timing time is ∞, if start/stop keyis pressed, it will display “CON” with flickering .c) In multi-points program, do not set the time to “OFF” in any section.4. Short MixThe device mixes at the frequency visible in thedisplay for as long as the Short Mix key is helddown. The time is counted in seconds until 999Short Mix is running600rpm 023Sseconds has expired.The max. short mix speed can be set accordingto your requirement at the current section.5. Temperature calibrationThe temperature of the Instrument has been adjusted before it is sold out. But if there isdeviation between the actual temperature and the displayed temperature due to somereasons, you can do as follows to correct the error.Notes: The Instrument uses three temperatures adjustment to ensure its veracity. Thismeans it is linearly adjusted on 10℃, 40℃ and 100℃ three points. The temperature veracitywill be within ±0.5℃ after the double temperature adjustment.Both the circumstances and the block temperature should be lower than 25℃.Adjustment methods as follows:a) After the startup of the Instrument, it enters waiting interface. Make sure thetemperature in display is below 25℃. If the temperature is higher than 25℃, you shouldwait until the temperature is below 25℃.— 8 —

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guideb) Inject olefin oil into one of the cone-shaped wells, and then put a thermometer intothis well (Make sure the precision of the thermometer should be within 0.1℃ and thetemperature ball should be absolutely immerged into the cone-shaped well). Heatinsulation material is needed on the block to separate it from the circumstance.Seeing from Fig a.:ThermometerAdiabatic BoardOlefin oilcone-shapedFig aNotes: Please read the actual value after 20minutes’ constant temperature to ensure theadjustment precision.c) Pressing seg’s“”and“”simultaneously,practical temperature shows 20.5, and rise to10.0 at once,at the same time the sign“*”flicker ceaselessly. When the practicaltemperature reach 10.0, the sign “ADJ” and“*” flicker ceaselessly togetherP:20.5AdjTemp=ADJ 10.0P:40.1AdjTemp=ADJ 10.0— 9 —

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guided) After 20 mintues,the actual temperature ofThermometer is 9.8℃.Pressing temp’s“”or“”key ,amend the value of “Adjtemp” toreach 9.8, then pressing “stop/start”key toconfirm.P:10.1 ADJ AdjTemp= 9.8Press “stop/start”Then rise to 40.0℃ automatically and “*”flicker ceaselessly.P:35.1 ADJ AdjTemp= 40.0e) When the practical temperature reach 40.0,the sign “ADJ” and “*” flicker ceaselesslytogether.P:40.1 ADJ AdjTemp= 40.0f) After 20 mintues,the actual temperature ofThermometer is 38.0℃.Pressing temp’s“”or“”key ,amend the value of “Adjtemp” toreach 38.0. then pressing “stop/start”key toconfirm.P:40.0 Adj AdjTemp= 38.0Press “stop/start”Then rise to100.0℃ automatically and “*”flicker ceaselessly.g) When the practical temperature reach 100.0,the sign “ADJ” and “*” flicker ceaselesslytogether.P:100.1 ADJ AdjTemp= 100.0h) After 20 mintues,the actual temperature ofThermometer is 98.0℃.Pressing temp’s“”or“”key ,amend the value of “Adjtemp” toreach 98.0. then pressing “stop/start”key toconfirm.P:100.0 Adj AdjTemp= 98.0Press “stop/start”— 10 —

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guideAfter Temperature calibration, the temperature displayed is the same with thepractical temperature of block.S1 30.0 0 00:00S1 50.0 1400 3:00Note!During Temperature calibration, press seg’s “”and“” simultaneously tocancel the calibration. The system keeps the former calibration.So don’ttemperature!press seg’s “”and“” simultaneously unless need calibrate the— 11 —

DBSC-001 Thermo Shaker Operations Manualchapter 4 Operation guide6.Exchange of blocka) Opens the transparent lid and pull out thefour screws which fix the block to theheating board with the screwdriver.Spannerb) Takes out the screws, closes the lid, putsout the block from the main enginec) Takes another model block, steadily laysaside on the main engine. The blockinstallment holes aim consistently with themain engine installment holes.d) Puts the screws into the installment hole,fixes the metal block on the instrument withthe spanner.Screw— 12 —

DBSC-001 Operations ManualChapter 5 Failure analysis and troubleshootingChapter 5 Failure analysis and troubleshootingFailure analysis and processing proceduresNo. Phenomenon Possible Causes Processing ProcedureNo powerCheck the power1No signals on thedisplay when it ispowered on.Broken FuseBroken switchExchange fuse(250V 3.0A5X20)Exchange the switchOthersContact to the seller234The actual anddisplayedtemperatures are quitedifferent.“OPEN” in thetemperature displaywith the alarm of“du...”“SHORT” in thetemperature displaywith the alarm of“du...”5 No heating or coolingBroken sensorTemperature sensor arebroken or the environmentaltemperature is below zeroTemperature sensor arebroken or the environmentaltemperature is below zeroBroken sensor or Broken TEmoduleContact to the sellerContact to the sellerContact to the sellerContact to the seller6 Press invalid Broken film switch Contact to the seller— 13 —

DBSC-001 Thermo Shaker Operations ManualannexAnnex 1 Wiring Diagram of DBSC-001 Thermo Shaker(This diagram is just for you reference. If there is any change, excuse for no notice.)LL10.5mm2 red24V PowerLPENPEEarthLPEN+-12V+12V-N0.5mm2 blue0.75mm2 black0.75mm2 redJ6PowerJ3Keypad interfaceJ6J3 16wireLcd DisplayTE0.75mm2 red0.75mm2 blackTE+TE-J6'J1PowerTE DriverLCD interfaceJ4 1x5wirekeyFan0.5mm2 red0.5mm2 blackFAN+FAN-J5Fan DriverMotor interfaceJ8motorPT1000.2mm2 white0.2mm2 whiteJ2Sensor— 14 —