Analysis of Fluorescence Decay by the Nonlinear Least Squares ...

RESULTS OF ANALYSIS BY
D a = 0.5 ns; ,=0.25 ns; FWHM = 0.9 ns
TABLE II
METHODS I AND II OF DATA SIMULATED USING
R(t) = W(e -e-t/0)
(AIIA2)* 7,* CH1SQ*
I II I II I II
10,0.2,1,2 10.07 ± 0.03 9.90 ± 0.03 0.198 ± 0.001 0.201 ± 0.001 1.05 ± 0.13 1.06 ± 0.15
1,0.2,1,2 1.03 ± 0.01 1.00 ± 0.01 0.195 ± 0.002 0.200 ± 0.002 1.12 ± 0.07 1.10 ± 0.06
10,0.1,1,2 10.21 ± 0.05 9.48 ± 0.05 0.098 ± 0.001 0.104 ± 0.001 1.12 ± 0.08 1.15 ± 0.07
1,0.1,1.2 1.05 ± 0.02 0.96 ± 0.02 0.096 ± 0.002 0.104 ± 0.002 1.07 ± 0.07 1.06 ± 0.08
10,0.05,1,2 10.40 ± 0.14 8.23 ± 0.07 0.048 ± 0.001 0.058 ± 0.001 1.09 ± 0.16 1.19 ± 0.14
1,0.05,1,2 1.13 ± 0.06 0.86 ± 0.03 0.045 ± 0.003 0.056 ± 0.002 1.03 ± 0.06 1.03 ± 0.07
10,0.02,1,2 10.85 ± 0.30 5.42 ± 0.05 0.018 ± 0.001 0.031 ± 0.001 1.02 ± 0.04 1.05 ± 0.03
1,0.02,1,2 1.27 ± 0.19 0.58 ± 0.03 0.017 ± 0.003 0.030 ± 0.003 1.01 ± 0.14 1.01 ± 0.14
E a = 1.0 ns; =0.5 ns; FWHM = 1.75 ns
10,0.1,1,2 10.10 ± 0.08 9.39 ± 0.06 0.099 ± 0.001 0.105 ± 0.001 1.16 ± 0.28 1.22 ± 0.29
1,0.02,1,2 1.40 ± 0.06 0.57 ± 0.06 0.017 ± 0.006 0.029 ± 0.006 1.09 ± 0.13 1.09 ± 0.13
F a = 2.5 ns; 3= 1.0 ns; FWHM = 4.0 ns
10,0.1,1,2 10.03 ± 0.09 9.33 ± 0.07 0.100 ± 0.001 0.106 ± 0.001 1.03 ± 0.05 1.05 ± 0.05
1,0.02,1,2 0.90 ± 0.22 0.54 ± 0.05 0.24 ± 0.005 0.031 ± 0.005 0.98 ± 0.04 0.98 ± 0.04
*Average and standard deviation of ten values.
$rl and T2 are in nanoseconds.
G y = 0.2 ns; FWHM =0.65 ns
TABLE III
RESULTS OF ANALYSIS BY METHODS I AND II OF DATA SIMULATED USING
R(t) = Wt2e-t'y
(AIIA2)* T,* CH1SQ*
I II I II I II
10,0.2,1,2 9.98 ± 0.04 9.82 ± 0.04 0.201 ± 0.001 0.204 ± 0.001 1.53 ± 0.15 1.86 ± 0.15
1,0.2,1,2 0.99 0.01 0.96 ± 0.01 0.203 ± 0.002 0.208 ± 0.002 1.33 ± 0.15 1.42 ± 0.16
10,0.1,1,2 9.87 ± 0.04 9.21 ± 0.04 0.102 ± 0.001 0.107 ± 0.001 1.54 ± 0.14 2.10 ± 0.18
1,0.1,1,2 0.94 + 0.02 0.87 ± 0.02 0.107 ± 0.002 0.115 ± 0.002 1.24 ± 0.06 1.33 ± 0.07
10,0.05,1,2 9.58 _ 0.10 7.82 ± 0.05 0.052 ± 0.001 0.061 ± 0.001 1.51 ± 0.20 2.04 ± 0.25
1,0.05,1,2 0.82 _ 0.02 0.69 ± 0.01 0.062 ± 0.002 0.072 ± 0.002 1.22 ± 0.12 1.28 ± 0.12
10,0.02,1,2 8.56 ± 0.19 4.98 ± 0.04 0.024 ± 0.001 0.035 ± 0.001 1.27 ± 0.16 1.42 ± 0.18
1,0.02,1,2 0.48 +0.04 0.36 ± 0.02 0.044 ± 0.003 0.055 ± 0.003 1.07 ± 0.08 1.09 ± 0.08
H y = 0.5 ns; FWHM =1.7 ns
10,0.1,1,2 9.86 ±0.09 9.21 ± 0.07 0.102 ± 0.001 0.107 ± 0.001 1.17 ± 0.09 1.28 ± 0.08
1,0.02,1,2 0.54 _ 0.11 0.36 ± 0.05 0.039 ± 0.008 0.049 ± 0.008 1.09 ± 0.12 1.09 ± 0.12
I y=0.1;FWHM=0.35ns
10,0.1,1,2 9.94 + 0.04 9.28 ± 0.03 0.101 ±_ 0.107 ± 2.34 ± 0.27 3.78 ± 0.38
1,0.02,1,2 0.52 ± 0.03 0.37 ± 0.01 0.040 ± 0.003 0.052 ± 0.003 1.26 ± 0.15 2.00 ± 0.08
*Average and standard deviation of ten values.
5Tl and T2 are in nanoseconds.
1Standard deviation is