PDMS Chip Interface - Dolomite Microfluidics
PDMS Chip Interface - Dolomite Microfluidics
PDMS Chip Interface - Dolomite Microfluidics
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<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong>product datasheetpageDescription 2Main Benefits 3Specification 4Cross-Sectional View 5Geometry 6Design Guidelines 8Fabrication Guidelines 10<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> Starter Kit 11Custom Options 11
The <strong>Dolomite</strong> Centre LtdPart namePart number<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> 3200232<strong>PDMS</strong> <strong>Chip</strong> Slide 50 x 25 mm (pack of 10) 3200264<strong>PDMS</strong> <strong>Chip</strong> Slide 75 x 25 mm (pack of 10) 3200275<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> Starter Kit 3200273DescriptionThe <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> (Part No. 3200232) allows users to create fast and reliablefluidic connections to <strong>PDMS</strong>-on-glass devices. The interface is designed to be used with a<strong>PDMS</strong> device (fabricated by the user) mounted to a <strong>PDMS</strong> <strong>Chip</strong> Slide with pre-drilledholes for fluidic connection (2 sizes available).Designing a <strong>PDMS</strong> device to fit the <strong>Dolomite</strong> format is quick and easy when following thedesign guidelines. Fabrication can be carried out using existing methods and equipment.The <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> is compatible with the Multiflux® Linear Connector 4-way (PartNo. 3000024) to provide 4 or 8 fluidic connections from 1/16” OD tubing to the <strong>PDMS</strong>chip.<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> (Part No. 3200232)<strong>PDMS</strong> <strong>Chip</strong> Slide (Part No. 3200275)Full assembly of the <strong>PDMS</strong> <strong>Chip</strong> with two<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong>s and two LinearConnectors 4-way (Part No. 3000024)Full assembly of the <strong>PDMS</strong> <strong>Chip</strong> with one<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> and one LinearConnector 4-way (Part No. 3000024)<strong>PDMS</strong>_chip_interface_product_datasheet Page 2 of 12
The <strong>Dolomite</strong> Centre LtdMain Benefits• Robust and reliable connections• Provides a standard format for <strong>PDMS</strong> chip connections• Ideal for research use• Time savings in fabrication: no requirement to punch holes in <strong>PDMS</strong>• Time savings in use: no downtime due to leaks• Extends chip lifetimes• Widens applications for <strong>PDMS</strong> chips outside of research labs• Quick connect/disconnect• Low dead volume• Good microscope access• Accommodates wide range of <strong>PDMS</strong> device heights<strong>PDMS</strong>_chip_interface_product_datasheet Page 3 of 12
The <strong>Dolomite</strong> Centre LtdSpecificationSpecification <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> (Part No. 3200232)Number of fluidic connections 4 or 8*Compatible glass slide dimensions 50 x 25 x 1.3mm or 75 x 25 x 1.3mm (L x W x H)Glass slide hole size<strong>PDMS</strong> chip thickness options<strong>PDMS</strong> chip width options<strong>PDMS</strong> chip length optionsOutside diameter of connection tubingInside diameter of connection tubingConnection tubing materialOperating pressureOperating temperature range of sealWetted materialsØ0.5mmUp to 6mmUp to 15mm10 – 44mm (50 x 25mm slide)10 – 69mm (75 x 25mm slide)1.6mm (1/16inch)0.25, 0.5 or 0.8mmPTFE, FEP5bar**-15 to 150°C***Glass, <strong>PDMS</strong>, PTFE (or FEP), and perfluoroelastomerConnector materials PEEK, Stainless Steel 316*8 fluidic connections requires 2 x <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> (Part No. 3200232) and 2 x Multiflux®Linear Connector 4-way (Part No. 3000024)**Based on tests at 21C, <strong>PDMS</strong> chip may delaminate at lower pressure***Assumes external surfaces of connector are at room temperature and tube I.D.
The <strong>Dolomite</strong> Centre LtdCross-sectional viewLinear Connector 4-way(Part No. 3000024)Glass slide 50 x 25 mm(Part No. 3200264)Left: Cross-sectional viewwith <strong>PDMS</strong> <strong>Chip</strong> Slide 50 x 25mm (Part No. 3200264)Below: Cross-sectional viewwith <strong>PDMS</strong> <strong>Chip</strong> Slide 75 x 25mm (Part No. 3200275)<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> (Part No. 3200232)<strong>PDMS</strong> microfluidic deviceLinear Connector 4-way(Part No. 3000024)Glass slide 75 x 25 mm(Part No. 3200275)<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> (Part No. 3200232)<strong>PDMS</strong> microfluidic devicePTFE or FEP tubingPerfluoroelastomer sealHole through glass slideDetailed View of Sealing <strong>Interface</strong>Microchannel in <strong>PDMS</strong><strong>PDMS</strong>_chip_interface_product_datasheet Page 5 of 12
The <strong>Dolomite</strong> Centre LtdGeometryGeometry of <strong>PDMS</strong> <strong>Chip</strong> with one <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> and one Linear Connector 4-way (Part No. 3000024)<strong>PDMS</strong>_chip_interface_product_datasheet Page 6 of 12
The <strong>Dolomite</strong> Centre LtdGeometry of <strong>PDMS</strong> <strong>Chip</strong> with two <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong>s and two Linear Connectors 4-way (Part No. 3000024)<strong>PDMS</strong>_chip_interface_product_datasheet Page 7 of 12
The <strong>Dolomite</strong> Centre LtdDesign GuidelinesIn order to design a compatible <strong>PDMS</strong> device for use with the <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong>, it isnecessary to follow 5 simple steps:1. Decide if you require 4 fluidic connections or 8 fluidic connections.a. 4 connections can be made with one <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> and one LinearConnector 4-way. Two chips can be mounted to each slide.b. 8 connections can be made with two <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong>s and two LinearConnectors 4-way. One chip can be mounted to each slide.2. Decide if your device length fits onto the standard 50 x 25 mm slide or if yourequire the longer 75 x 25 mm slide.3. Design pad features in <strong>PDMS</strong> (recommended Ø1 mm) at a pitch of 3 mm to alignwith Ø0.5 holes in glass slide. These are the inlet and outlet ports.4. Design outer dimensions of <strong>PDMS</strong> device ensuring that the boundaries are withinthe maximum width and length specified.a. Maximum width is 15 mm. The maximum distance from outside port to<strong>PDMS</strong> side edges is 3 mm.b. Maximum length depends on the number of fluidic connections (step 1)and the slide size selected (step 2). The maximum distance from inlet andoutlet ports to <strong>PDMS</strong> back edge is 3 mm.5. Design channel features in <strong>PDMS</strong> ensuring that all features to be viewed arepositioned at least 3 mm from the inlet and outlet ports. Visible features should beoutside of the red box in the diagram below.<strong>PDMS</strong>_chip_interface_product_datasheet Page 8 of 12
The <strong>Dolomite</strong> Centre LtdKey design dimensions of <strong>PDMS</strong> replica if 4 fluidic connections are required and2 chips of equal length are mounted onto the <strong>PDMS</strong> <strong>Chip</strong> SlideKey design dimensions of <strong>PDMS</strong> replica if 8 fluidic connections are required (1 chipmounted onto <strong>PDMS</strong> <strong>Chip</strong> Slide)<strong>PDMS</strong>_chip_interface_product_datasheet Page 9 of 12
The <strong>Dolomite</strong> Centre LtdFabrication GuidelinesFabrication of the <strong>PDMS</strong> device can be carried out using existing soft lithographicmethods and equipment:1. Fabricate a photoresist master (spin coat photoresist onto a silicon wafer, exposeand develop).2. Degas the <strong>PDMS</strong> mixture. Then cast the <strong>PDMS</strong> onto the master, cure and peelthe wafer of <strong>PDMS</strong> replicas from the photoresist master. The <strong>PDMS</strong> height shouldbe no greater than 6 mm.3. Dice the wafer of <strong>PDMS</strong> replicas into individual chips ensuring that outerdimensions are within the allowed size.4. Ensure that bonding surfaces of the glass and <strong>PDMS</strong> are clean then expose bothsurfaces to O 2 plasma.5. Align <strong>PDMS</strong> pad features to holes in the glass slide by eye or using lowmagnification eyepiece or viewer. Apply light pressure to create bond.6. The completed <strong>PDMS</strong> chip can then be used with the <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong>.Described above is a typical soft lithography process, but alternative methods may beused for the creation of channel features in the <strong>PDMS</strong>.Example of <strong>PDMS</strong> replica mounted onto <strong>PDMS</strong> <strong>Chip</strong> Slide<strong>PDMS</strong>_chip_interface_product_datasheet Page 10 of 12
The <strong>Dolomite</strong> Centre Ltd<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> Starter KitThe <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> Starter Kit (Part No. 3200273) contains the requiredcomponents to start using the Multiflux® <strong>PDMS</strong> chip connection system.The starter kit includes:• 1 x <strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> – Part No. 3200232• 1 x <strong>PDMS</strong> <strong>Chip</strong> Slide 50 x 25mm (pack of 10) – Part No. 3200264• 1 x Multiflux® Linear Connector 4-way – Part No. 3000024• 1 x Fluid Tube PTFE 1/16” OD, 0.5mm I.D, 10m Length – Part No. 3200067<strong>PDMS</strong> <strong>Chip</strong> <strong>Interface</strong> Starter Kit (Part No. 3200273)Custom Options<strong>Dolomite</strong> can offer custom connection systems for individual applications including thinlayer glass slides for high magnification microscopy. Microscope holders are alsoavailable on request. The range of <strong>Dolomite</strong> services available covers all aspects of thedevelopment process from concept studies and feasibility testing to the design ofcommercial instruments. Please contact <strong>Dolomite</strong> to discuss your application.<strong>PDMS</strong>_chip_interface_product_datasheet Page 11 of 12
The <strong>Dolomite</strong> Centre Ltd<strong>PDMS</strong>_chip_interface_product_datasheet Page 12 of 12