Lambda 20/Lambda 40 Installation and Maintenance Guide - VTP UP

Basic InstallationThis completes the coarse alignment of the cell holder. If necessary, proceed withthe fine alignment as described below.Fine AlignmentIf fine alignment is necessary, proceed as follows:1. Using the [GOTO] key, slew the monochromator to your measurementwavelength or to 460 nm.2. Call up a method that uses transmission (%T) as the ordinate.If necessary change the ordinate mode to transmission.3. Open the sample compartment cover.4. Insert the cell with a low absorbing solvent into the sample cell holder.Leave the reference cell holder empty.5. Make horizontal fine alignment to the sample cell holder (locking screws andmilled posts loosened) to obtain the highest possible transmittance reading onthe display (close sample compartment cover while measuring transmittance).6. Make vertical fine adjustment using the vertical adjustment screw again toobtain the highest possible reading (close sample compartment cover whilemeasuring transmittance).7. When you are satisfied with the alignment, tighten the milled posts and thelocking screws on the cell holder.8. Insert the matching cell with the same low absorbing solvent into the referencecell holder.The first cell remains in the sample cell holder.9. Repeat steps 5 to 7 with the reference cell holder, but this time obtain thelowest possible transmittance reading on the display.This completes the fine alignment procedure.33