Lambda 20/Lambda 40 Installation and Maintenance Guide - VTP UP

Basic Installation3. Move the milled posts a little to locate the threaded holes in the baseplate, andthen tighten the milled posts.The tube ports located at the front of the sample compartment allow you to leadtubes from flowcells, water-thermsotatted cell holders, etc. in and out of thesample compartment.When not in use, you should always insert the caps into the ports.Aligning the Single Cell HolderCoarse alignment of the single cell holder is carried out as follows:1. Open the sample compartment cover.2. Fill matching cells with a low-absorbing solvent (deionized water or ethanol).3. Insert one cell into the sample cell holder and one into the reference cell holder.Make certain that the cell is pushed down fully.NOTE: The alignment procedure is for a given cell in a given holder. After alignment, thecell should always be used in the same holder.4. Block the sample and reference beam window on the right hand side of thesample compartment with a card to prevent white light from saturating thedetector.5. Return to standby display.6. Using the [GOTO] key, slew the monochromator to 0 nm to obtain a beam ofvisible (zero order) radiation in the sample compartment.7. By holding a piece of matt white paper behind each cell holder, visuallyexamine the light spot to see that the radiation beam is passing through the cellsample area.Diffraction patterns become apparent if the radiation beam impinges on the cellwall.31