FA 5 Progress Report WV-INBRE - Joan C. Edwards School of ...

Program Director/Principal Investigator (Last, First, Middle): Rankin, Gary O 81Graduate Students No NoUndergraduate StudentsWVU Microscope Imaging FacilityThe Microscope Imaging Facility provides resources for light microscopy image acquisition, as well asimage processing and analysis, on a fee-per-use basis. This shared resource is available to all researchersacross the university and the state. The facility currently has eight microscopes, two of which were addedduring the previous year. One is a new fully automated Zeiss AxioImager that is equipped forepifluorescence, brightfield, darkfield and DIC imaging. This system was purchased with funds provided bythe WV-INBRE grant (P20 RR016477). The second new system is a Zeiss Axiovert 40 CFL tissue culturemicroscope for widefield fluorescence and phase contrast imaging. The facility also has two laser scanningconfocal systems, an inverted Zeiss LSM 510 confocal system with 3 lasers (488, 514, 543 and 633nm) andan upright Zeiss LSM 510 confocal with four lasers (405, 458, 477, 488, 514, 543 and 633nm). Thesesystems are used for experiments such as multi-color fluorescent imaging, 3D rendering and FRAP. Anupright Olympus AX70 brightfield/epifluorescent microscope equipped with the MicroBrightField Neurolucidaand Stereo Investigator software packages is available for color histology records, slide scanning and serialsection reconstruction. For microdissection, the facility has a Zeiss PALM MicroBeam system fornon-contact microdissection of single cells or groups of cells. The isolated cells can then be used for DNAor RNA analysis, proteomic analysis or for further cultivation. For live-cell imaging, the facility has a NikonTE2000S epifluorescent microscope with Prior filter wheels and a Photometrics Coolsnap HQ CCDcamera. This microscope also has an Eppendorf FemtoJet microinjection system and the MetaMorph andMetaFluor software packages. This system is used for multi-color time-lapse, FRET and calcium ratioimage acquisition. For high-end live-cell imaging, the facility has a new Nikon Swept-Field Confocal systemwith 3 solid-state lasers (491, 561 and 638nm). This system uses a Photometrics QuantEM CCD camerawith on-chip gain to maximize signal and to increase the rate of acquisition. This system is also equipped forepifluorescence, DIC, phase contrast and laser TIRF acquisition using a high resolution Photometrics Cool-Snap HQ2 CCD camera. A Prior ProScan II motorized stage supports multipoint acquisition, whichhas increased throughput from single experiments. Environmental control (OKO Labs Stage Top Incubatorwith temperature, humidity and CO2 control, a Bioptechs Delta T4 dish heater and objective heaters and amicro-perfusion system) and a fully equipped tissue culture facility including an incubator are available tomaintain live cell cultures. Two off-line image analysis workstations are available to the facility users forimage processing and data analysis. Software packages include AutoQuant (deconvolution), NIS-Elements(2D tracking, ratio and FRET analysis and 3D rendering), Zeiss AxioVision, Photoshop and Image J. Thefacility staff are dedicated to providing ongoing training and support to ensure the success of imagingprojects. The Microscope Imaging Facility has been supported by the Mary Babb Randolph Cancer Centerand NIH grants P20 RR016440, P30 RR032138/GM103488 and P20 RR016477.Users Unique # Fees Charged Fees Paid by CenterFaculty/Post Doctorate/Staff Sometimes NoGraduate Students No NoUndergraduate Students No NoMU Genomics Core FacilityMarshall University Genomics Core Facility:NoNoNext generation sequencing and microarray experimentation require sophisticated methods, expensiveinstrumentation and reagents, and intense data management and analysis. These tasks are most efficientlyexecuted in a core facility environment. The primary objective of the MU Genomics Core Facility is to enablethe genomic research goals of the INBRE, COBRE and other research projects at WV universities andcolleges. The Genomics Core Facility currently provides the following services: (1) high throughput nextgeneration sequencing (NGS), (2) microarray-based gene expression profiling and statistical support, (3)automated capillary DNA sequencing and access to DNA sequence analysis software (4) access toreal-time thermal cyclers for quantitative PCR and to Agilent 2100 Bioanalyzers for DNA/RNA quantitationand quality assessment, and (5) purification and banking of patient genomic DNA.PHS 2590 (Rev. 06/09)Continuation Format Page