Program Director/Principal Investigator (Last, First, Middle): Rankin, Gary O 78extraction, sample injection and running the HPLC for ATP analysis. These students are becomingmore pr<strong>of</strong>icient every day at conducting Western blot analysis. Michael Wright is independently ableto conduct our protein analysis, gel preparation and antibody applications for western analysis.We are currently accepting applications for the full-time technician position in the laboratory <strong>of</strong> Dr.Valentovic. Currently there are 8 applicants have applied for the position. It is hoped that interviewand hiring can be completed by March 1.PROTECTION AGAINST RESEARCH RISKSN 1. Will human subjects be involved next year?Y 2. Will vertebrate animals be used next year?If yes, provide the date <strong>of</strong> Institutional Animal Care and Use Committee (IACUC) approval and enclosewith transmittal.02/06/2009If no approval date, please explain.N Is this IACUC approval date different from the date reported last year?N 3. Will recombinant DNA experiment(s) be conducted next year?N 4. Are there potential hazards to laboratory workers (carcinogens, pathogens, ionizing radiation, etc.)involved in the proposed research for next year? If yes, identify:N5. Will any <strong>of</strong> the research-risk categories,not involved next year, be involved future years? If yes, identify:PHS 2590 (Rev. 06/09)Continuation Format Page
Program Director/Principal Investigator (Last, First, Middle): Rankin, Gary O 79SHARED <strong>FA</strong>CILITIES<strong>WV</strong>U Flow Cytometry Core FacilityThe <strong>WV</strong>U Flow Cytometry Core Facility provides instrumentation and scientific support for cell analysis andsorting. The facility routinely performs analysis <strong>of</strong> both eukaryotic and prokaryotic cells for expression <strong>of</strong>intracellular and extracellular proteins, cell cycle, cell quantitation, cytokine production, and cell sorting basedon antigen expression or cell cycle. The facility is currently equipped with two cytometers, a BectonDickinson (BD) <strong>FA</strong>CSAria and a BD <strong>FA</strong>CSCalibur. The <strong>FA</strong>CSAria is a 15 parameter (13 fluorescent markersand two scatter parameters) high-speed bench top sorter capable <strong>of</strong> sorting cells into 4 different populations.Using a grant from the <strong>WV</strong>-<strong>INBRE</strong>, the Facility will purchase and install a biological safety cabinet (BSC) thatis specially designed for the sorter prior to April 30, 2012. The BSC will replace the current aerosolcontainment system and enable the Facility to sort infectious agents or cells <strong>of</strong> human origin under BiosafetyLevel-2 conditions, which has become the recommended standard for these activities. The dual laser<strong>FA</strong>CSCalibur is capable <strong>of</strong> 4-color analysis and is equipped with a sample autoloader. In addition to the twocytometers, the facility has a Miltenyi Biotec AutoMACS and tissue culture hood for sterile magnetic cellsorting. The facility has auxiliary laboratory space with appropriate equipment to train personnel and supportthe needs <strong>of</strong> researchers. The Flow Cytometry Core has several s<strong>of</strong>tware packages including FCS Express,CellQuest Pro, ModfitLT and BD Diva s<strong>of</strong>tware for data acquisition and analysis. It also has two separateanalysis workstations with 2 print quality color laser printers. The facility is managed by the technical directorKathleen Brundage, PhD who has training from BD biosciences and many years <strong>of</strong> experience with flowcytometry. She oversees the day-to-day operations <strong>of</strong> the facility, operates the <strong>FA</strong>CSAria for cell sorting,provides necessary instrument and s<strong>of</strong>tware training, and consults onexperimental design and data analysis. The facility is house in an approximately 750 sq ft lab space locatedon the 2nd floor <strong>of</strong> the Robert C. Byrd Health Sciences Center North building.Users Unique # Fees Charged Fees Paid by CenterFaculty/Post Doctorate/Staff Sometimes NoGraduate Students No NoUndergraduate Students No No<strong>WV</strong>U Genomics CoreThe <strong>WV</strong>U Genomics Core Facility <strong>of</strong>fers full-service gene expression pr<strong>of</strong>iling and DNA sequencing for allinvestigators in West Virginia. For researchers without expertise in these laboratory-based techniques, thecore provides full gene expression services, including RNA purification from provided tissue samples,assessment <strong>of</strong> RNA quality, amplification (if necessary), probe synthesis and labeling, array hybridization,and multi-level bioinformatics analysis. This breadth <strong>of</strong> service provides accessibility to gene expressionpr<strong>of</strong>iling for many different types <strong>of</strong> investigators. For investigators more adept at molecularcharacterizations, a limited subset <strong>of</strong> these assays could be performed by the core facility personnel.Similar varied service levels are <strong>of</strong>fered for DNA sequencing. The Director and Laboratory Manager forMicroarray/Gene Expression are available prior to the start <strong>of</strong> experiments to ensure that appropriate designmeasures are included for statistically significant microarray findings. The Co-Director and LaboratoryManager for DNA Sequencing provide technical and bioinformatics consulting for users <strong>of</strong> those services.Interactions between investigators and the core staff through every step <strong>of</strong> the design and execution <strong>of</strong> theresearch study will facilitate better and faster scientific discovery and make these core services far moreconvenient and effective than commercial vendors. The core facility also assists investigators in placingdatasets into public databases and maintains these datasets on Genomics Core Facility servers for accessby the scientific community at large.Users Unique # Fees Charged Fees Paid by CenterFaculty/Post Doctorate/Staff No NoGraduate Students No NoUndergraduate Students No NoPHS 2590 (Rev. 06/09)Continuation Format Page
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