ARI Volume 2 Number 1.pdf - Zoo-unn.org

ARI Volume 2 Number 1.pdf - Zoo-unn.org ARI Volume 2 Number 1.pdf - Zoo-unn.org

12.07.2015 Views

Animal waste management strategies 269Increase digestible P (g/Kg)1.41.210.80.60.40.20Corn/soy dietBy-product diet0 450 900 1350 1800 2250Phytase (FTU/Kg)Figure 1: Improvement in digestible P bymicrobial phytase (Natuphos) in two dietsfor growing pigs (Beers and Jongbloed,1992)(based either on corn-soybean meal or phytaterichby-products). The efficiency of microbialphytase appeared to be related to its dose and thetype of diet (Figure 1).From 0 – 400 FTU/Kg there was a rapidincrease in microbial phytase efficacy, whichflattened afterwards. In the experiment, it wasshown that microbial phytase was considerablyeffective in enhancing phosphorus digestion andso increases the amount of digestible /availablephosphorus in the feed for pigs.Approximately 67 % of the phosphorus in planttissue is in the form of phytate phosphorus(Myoinositol hexakisphosphate) (Cromwell et al.,1993), which is only minimally available tomonogastric animals since they lack the phytaseenzyme that hydrolyses phytic acid to inositoland/or thophosphate (Peeler, 1972).Supplementation of phytase enzyme in cornsoybeanmeal diet of broiler chickens improves theavailability of phytate bound phosphorus (Simonset al., 1992; Edens et al., 1999). Phytatephosphorus content of corn is 68% of the totalphosphorus, and in soybean meal phytatephosphorus represents 60 % of the totalphosphorus (Edens et al. 1999). Simons et al(1990) demonstrated that in three-week-oldbroilers the availability of dietary phosphorus couldbe increased up to 65 % by means ofsupplemental dietary phytase while reducing fecalphosphorus by 50 %. Furthermore, inclusion ofphytase activity in diets having wheat, triticale,rye, or their by-products resulted in betterphosphorus utilization in poultry (Choct, 2001).The efficacy of microbial phytase depends onanimal related factor such as physiological statusand housing condition (Kemme et al. 1997a).Kemme et al. (1997b) showed that the efficacy ofphytase in generating digestible phosphorusdecreased in the order of lactating sows, growingfinishingPigs, sows at the end of pregnancy,piglets and sows at mid pregnancy.A prerequisite for a good evaluation ofmicrobial phytase efficacy is that the animal be fedbelow their phosphorus requirement. This is due tointestinal regulation of phosphorus absorptionwhen animals are fed above their phosphorusrequirement. It is commonly known that higherdietary calcium levels decrease apparentabsorption of phosphorus. Thus a balance ofcalcium: phosphorus must be established forexcellent performance (Jongbloed, 1993).Reducing Nitrogen Excretion throughNutrition: To reduce the level of nitrogen in fecalmatter through nutrition, two approaches arepossible thus: (I) Enhancement of the depositionof nitrogen in animal products (meat, eggs, milk)and (ii) constant maintenance reduction of dietarynitrogen input while productivity is sustained(Tamminga and Verstegen, 1992). The firstapproach requires the intermediary metabolism tooperate more efficiently, while the secondapproach largely depends on reducing nitrogenlosses along the gastro-intestinal tract.Both approaches will result in a reductionof Nitrogen in animal excreta by 20 %. A moreefficient intermediary metabolism will reducenitrogen excretion in urine by 10 %, whereas areduction in losses from gastro-intestinal willreduce quantities present in both faeces and urineby 30 % (Lenis and Jongbloed, 1994). Feedingration containing a poor balance of amino acidsresults in removal of excess nitrogen in the faeces.Taylor, et al. (1979) indicated that the dietarycontent of total crude protein could be reducedfrom 17.6 % to 14.5 % by the addition ofcrystalline lysine. This leads to improved balanceof essential amino acids present in the diet andbetter protein utilisation.Inclusion of protease in the diet may alsopromote more efficient utilization of dietary protein(Headon and Walsh, 1994). The endogenousproteolytic activities associated with the digestivetract are normally more than adequate to promoteefficient degradation of dietary protein. Forinstance supplementation of feeds with exogenousmicrobial proteolytic activities can serve to improveprotein utilization in animals subject to highprotein intake.Cellulase help in the breakdown of cellwall structure and make nutrients in vegetablematerials much more available to the animal, theyalso break down xylan in cereal grains and reduceviscosity of the digester. The increase in the transittime in the gut leads to increase efficiency ofutilization. The enzyme activation may also help

UCHEWA, Emmanuel Nwafoagu et al. 270promote more efficient digestion of poorlydigestible proteins, such as those found in intimateassociation with some other dietary factors(Bateman 1998).In monogastric animals, proteindigestibility is low for some legume seeds, due tothe presence of anti-nutritional factors (ANF) likelectins and protease inhibitors (Tamminga andVerstegen 1994). Low protein digestion can beovercome by technological treatment of the diet inan optimal combination of temperature, moistureand time. Short treatment at high temperature ismore effective in reducing the antinutritional factorcontent of the dietary ingredient. Reduction in theactivity of proteinous ANF and further breakdownof non-starchy polysaccharides can beaccomplished using enzymes, during germinationand grinding to finer particle size.Beal et al. (1998) in a factorial analysisdemonstrated the difference in the in vitronitrogen digestibility between raw soybean anddifferent full fat soybean meals both with andwithout enzyme treatment at different p H (Table1). Surprisingly, they observed that raw soybeanappeared to be more digestible in pigs thanprocessed soybean meal. However, the pretreatmentof soybean with exogenous enzymesincreases protein digestibility. This is because largemolecular weight proteins are partially hydrolyzedbefore the commencement of digestion. Thedifference in nitrogen digestibility between the rawand processed soybean meal could be due to anumber of factors. Heat denaturation preventingdigestive enzymes to act on amino acid residues,differences in solubility due to the pH of thestomach and loss of available protein due to heatinduced interactions with other substances.Nitrogen excretion can also be reducedsubstantially by supplying dietary amino acids inaccordance with the animal’s requirement and byincorporating free amino acids in the feeds andlowering crude protein content.Multi phase feeding, in which diets can beautomatically adjusted by means of a computercontrolled feeding system may reduce excretion ofnitrogen and phosphorus by 10 and 15 to 22%,respectively. Bourdon and others achievednitrogen and phosphorus reduction applying multiphase feeding to castrated male pigs weighingbetween 25 and 100kg with decreased dietaryprotein levels, and supplementary addition oflimiting amino acids (VanKlooseter et al., 1998).From the experiments they concluded that theamount of nitrogen excreted was reduced by 50%, with multi phase feeding accounting for 0.10%. Van-der Peet-schwering et al. (1996) usingmultiphase feeding for growing and castrated malepigs between 25 and 110Kg live weight. Reportedthat multi phase feeding reduced ammoniaemission by 45%, compared to the single controldiet. Further more, multi phase feeding lead to 22% reduction in phosphorus excretion by growingpigs (Beers and Jongbloed 1992). Results ofKemme et al. (1997a, b) indicated that multi phasefeeding does not always lead to optimumperformance and slaughter quality of pigs.Requirements of nitrogen and phosphorusfor breeding sows are much lower duringpregnancy than during lactation. The use ofseparate diets for pregnancy and lactationcompared with one diet for both reduced theexcretion of nitrogen and phosphorus by 20 %(VanKlooseter et al., 1998).Growth promoters, because of improvedfeed conversion ratios have an estimated 7 and3% reduction on nitrogen and phosphorusexcretion per weaned piglet and growing pigrespectively, according to Jongbloed et al (1992).Both nitrogen and phosphorus excretion can befurther reduced with recombinant porcinesomatotropin (rPST) (Bateman 1998);unfortunately, the use of growth promoters in feedis banned.The source and level of fermentablecarbohydrates in the diet influence ammoniavolatilisation of pig slurry (Coppoolse et al. 1990).In an experiment, using three different treatments,Aarnink and Lenis (1998) fed soluble maize starchin treatment 1 and replaced it with coconutexpeller and soybean hulls in treatment II and IIIrespectively, ammonia volatilisation was decreasedunder laboratory conditions by 0.35%, 0.51% and0.36% respectively. In a second experiment, thesame authors examined the effect of electrolytebalance (Na + K – Cl), Ca-level and Ca-salt onammonia emission from slurry. When CaSO4, Ca –benzoate or CaCl2, replaced CaCO3 respectively,the ammonia emission of slurry under laboratoryconditions was reduced by 30%, 54% and 33%respectively.EFFECT ON THE PERFORMANCE OF THEANIMALThere have been numerous reports on the effectsof microbial phytase and phosphorus utilization. Inaddition to the general established improvementsin phosphorus digestibility, significantly higher liveweight gain and better-feed conversion efficiencyhave often been reported (Beers and Jongbloed,1992; Cromwell, 1980; Dungelhoef andRodehutscord, 1995 and Kemme et al., 1997a).This could be explained by interference of phyticacid with the digestion of other essential mineralsand protein. Phytate complexes in acid andalkaline media have been described (Dierick andDecuypere (1994). In vitro studies have shownthat phytate-protein complexes involving aminogroups of lysine, histidine and arginine are formed,which are insoluble and biologically unavailable in

Animal waste management strategies 269Increase digestible P (g/Kg)1.41.210.80.60.40.20Corn/soy dietBy-product diet0 450 900 1350 1800 2250Phytase (FTU/Kg)Figure 1: Improvement in digestible P bymicrobial phytase (Natuphos) in two dietsfor growing pigs (Beers and Jongbloed,1992)(based either on corn-soybean meal or phytaterichby-products). The efficiency of microbialphytase appeared to be related to its dose and thetype of diet (Figure 1).From 0 – 400 FTU/Kg there was a rapidincrease in microbial phytase efficacy, whichflattened afterwards. In the experiment, it wasshown that microbial phytase was considerablyeffective in enhancing phosphorus digestion andso increases the amount of digestible /availablephosphorus in the feed for pigs.Approximately 67 % of the phosphorus in planttissue is in the form of phytate phosphorus(Myoinositol hexakisphosphate) (Cromwell et al.,1993), which is only minimally available tomonogastric animals since they lack the phytaseenzyme that hydrolyses phytic acid to inositoland/or thophosphate (Peeler, 1972).Supplementation of phytase enzyme in cornsoybeanmeal diet of broiler chickens improves theavailability of phytate bound phosphorus (Simonset al., 1992; Edens et al., 1999). Phytatephosphorus content of corn is 68% of the totalphosphorus, and in soybean meal phytatephosphorus represents 60 % of the totalphosphorus (Edens et al. 1999). Simons et al(1990) demonstrated that in three-week-oldbroilers the availability of dietary phosphorus couldbe increased up to 65 % by means ofsupplemental dietary phytase while reducing fecalphosphorus by 50 %. Furthermore, inclusion ofphytase activity in diets having wheat, triticale,rye, or their by-products resulted in betterphosphorus utilization in poultry (Choct, 2001).The efficacy of microbial phytase depends onanimal related factor such as physiological statusand housing condition (Kemme et al. 1997a).Kemme et al. (1997b) showed that the efficacy ofphytase in generating digestible phosphorusdecreased in the order of lactating sows, growingfinishingPigs, sows at the end of pregnancy,piglets and sows at mid pregnancy.A prerequisite for a good evaluation ofmicrobial phytase efficacy is that the animal be fedbelow their phosphorus requirement. This is due tointestinal regulation of phosphorus absorptionwhen animals are fed above their phosphorusrequirement. It is commonly known that higherdietary calcium levels decrease apparentabsorption of phosphorus. Thus a balance ofcalcium: phosphorus must be established forexcellent performance (Jongbloed, 1993).Reducing Nitrogen Excretion throughNutrition: To reduce the level of nitrogen in fecalmatter through nutrition, two approaches arepossible thus: (I) Enhancement of the depositionof nitrogen in animal products (meat, eggs, milk)and (ii) constant maintenance reduction of dietarynitrogen input while productivity is sustained(Tamminga and Verstegen, 1992). The firstapproach requires the intermediary metabolism tooperate more efficiently, while the secondapproach largely depends on reducing nitrogenlosses along the gastro-intestinal tract.Both approaches will result in a reductionof Nitrogen in animal excreta by 20 %. A moreefficient intermediary metabolism will reducenitrogen excretion in urine by 10 %, whereas areduction in losses from gastro-intestinal willreduce quantities present in both faeces and urineby 30 % (Lenis and Jongbloed, 1994). Feedingration containing a poor balance of amino acidsresults in removal of excess nitrogen in the faeces.Taylor, et al. (1979) indicated that the dietarycontent of total crude protein could be reducedfrom 17.6 % to 14.5 % by the addition ofcrystalline lysine. This leads to improved balanceof essential amino acids present in the diet andbetter protein utilisation.Inclusion of protease in the diet may alsopromote more efficient utilization of dietary protein(Headon and Walsh, 1994). The endogenousproteolytic activities associated with the digestivetract are normally more than adequate to promoteefficient degradation of dietary protein. Forinstance supplementation of feeds with exogenousmicrobial proteolytic activities can serve to improveprotein utilization in animals subject to highprotein intake.Cellulase help in the breakdown of cellwall structure and make nutrients in vegetablematerials much more available to the animal, theyalso break down xylan in cereal grains and reduceviscosity of the digester. The increase in the transittime in the gut leads to increase efficiency ofutilization. The enzyme activation may also help

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