Automated Axon Tracking of 3D Confocal Laser Scanning ...

(e)Figure 10 – Tracking results in dataset one: (a) The MIP image of the dataset, (b) Cross-over in the MIP image(The region inside the yellow rectangle in (a) has been magnified for better visualization), (c) Manual trackingresults, (d) Centerlines in MIP image, and (e) 3D model of the centerlines. The scale bars in (a) and (d) correspondto 6μm. The scale bar in (b) corresponds to 4μm.Approximately 50% of the dataset was tracked using the cross-sectional tracking algorithm.The average deviation of the centerlines detected by our algorithm from the manually trackedresults was found to be 1.75 pixels.The second dataset contained six axons with 256 cross-sectional images, each having aresolution of 43 x 512 pixels. The cross-sectional images were sampled along the X direction.Figure 11(a) shows the MIP image for this dataset. The cross-over of the axons in the MIP imageis shown inside the white ellipse in Figure 11(b). The maximum width of the axon was set toforty pixels while the resolution of the grid for the MIP image was set to fifteen pixels. Figure11(c) shows the manual tracking results for the dataset. The 2D and the 3D centerlines are shownin Figure 11(d)-(e).26