Schriften zu Genetischen Ressourcen - Genres

F. MARTHE, W. SCHÜTZE, H. KRÜGER, P. SCHOLZE, R. KRÄMER and U. RYSCHKA
(CH3CN) (99:1; start conditions), 17.5 min: B 25%, 20.0 min: B 25%, 25.0 min: B 1%,
analytic time: 30 min, column temperature: 35°C, wave length: 229 nm, internal standard:
sinigrine; determination as desulfoglucosinolates. Flavonoids, HPLC-
Conditions: system: System 1100 (Hewlett Packard with DAD), column: ZORBAX SB
C18 150 x 2.1 mm; 3.5µ, flow: 0.50 ml/min, gradient: A (1% COOH )/ B (CH3CN)
(100:0; start conditions), 4.0 min: B 0%, 25.0 min: B 25%, 30.0 min: B 60%, 30.1 min:
B 60% flow: 0.70 ml/min, 35.0 min: B 0% flow: 0.70 ml/min, 40.0 min: B 0% flow: 0.50
ml/min, analytic time: 40 min, column temperature: 35°C, wave length: 288 nm. To
isolate the volatiles, an isooctane extract was prepared.
Resistances were tested by inoculations of plants with a suspension separately for
each pathogen: Alternaria brassicae, A. brassicicola, Phoma lingam and Plasmodiophora
brassicae. All tests were carried out in climate chambers under defined conditions
(18-23 °C, 16 h light, humid conditions 7 days past inoculation). The turnip mosaic
virus (TuMV) was tested by mechanical inoculation under green house conditions.
Plants were rated for disease reaction 4 weeks after inoculation by ELISA immunotest.
Results and discussion
Both accessions were grown from April to November in the experimental field. The
plant development was very slow – at the end of vegetation period the hypocotyl
bulbs reached 1 to 1.5 cm. No plant survived the winter outside. Plants which were
grown in the greenhouse needed a vernalisation period of about 12 weeks (0 - 4 °C,
12 h light). The flowers are self-fertile.
Fig. 1: HPLC-separation of glucosinolates from a root extract of maca and
UV-spectra
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