Schriften zu Genetischen Ressourcen - Genres
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Schriften zu Genetischen Ressourcen - Genres

Schriften zu Genetischen Ressourcen - Genres Schriften zu Genetischen Ressourcen - Genres

genres.de
30.11.2012 Views

E.R.J. KELLER, A. SENULA and H. SCHULZ Fig. 2: Relative percentages of some selected cysteine sulfoxides in the basal parts of the plants Note that the hybrid plants show intermediate behaviour Discussion and conclusions The genepool of wild Allium species accumulated in the Gatersleben collection provides a good background for % 90 80 70 60 50 40 30 20 10 0 A. obliquum Hybrid A. cepa methyl 2-propenyl 1-propenyl hybridisation programmes in breeding and basic research. Examples demonstrated in this study are an initial step for further strategies. Primary hybrids derived from distant crosses are usually seedsterile. Therefore, diploidisation of the material should be attempted to obtain presumably fertile amphidiploid plants. One of the options to get such material is in vitro colchicination. First hybrids have been treated with colchicine in our laboratory. A sample of 150 plants from in vitro colchicine treatment has been transferred into the field. First measurements by flow cytometry resulted in 131 plants with doubled chromosome sets. Once the plants come to the flowering stage, seed set will be the final prove of successful production of fertile plants. Management and sensible use of the genetic diversity within a group of crop plant species and their wild relatives offers a promising way to future use of the richness of their genepool. References EMSWELLER, S.L. and H.A. JONES (1935): An interspecific hybrid in Allium. - Hilgardia 9, 277-288. 277

Wild species as a source for introgression - the case of Allium FRITSCH, R.M. (2002): A short history of the taxonomic collection of genus Allium housed at the Institute of Plant Genetics and Crop Plant Research (IPK). In: L. MAGGIONI, J. KELLER and D. ASTLEY (compilers): European collections of vegetatively propagated Allium. Report of a Workshop, 21-22 May 2001, Gatersleben, Germany. International Plant Genetic Resources Institute, Rome, Italy. pp. 36- 43. KELLER, E.R.J., I. SCHUBERT, J. FUCHS and A. MEISTER (1996): Interspecific crosses of onion with distant Allium species and characterization of the presumed hybrids by means of flow cytometry, karyotype analysis and genomic in situ hybridization. - Theor. Appl. Genet. 92, 417-424. PETERKA, H., H. BUDAHN and O. SCHRADER (1997): Interspecific hybrids between onion (Allium cepa L.) with S-cytoplasm and leek (A. ampeloprasum L.). - Theor. Appl. Genet. 94, 383-389. SCHULZ, H., H. KRÜGER, N. HERCHERT and E.R.J. KELLER (2000a) Vorkommen flüchtiger Sekundärmetaboliten in ausgewählten Allium-Wildtypen. - J. Appl. Bot. 74, 119-121. SCHULZ, H., H. KRÜGER, N. HERCHERT, E.R.J. KELLER and M. KEUSGEN (2000b): Charakterisierung von Allium-Bastarden auf Basis der Aromaprofile. DGQ, XXXV. Vortragstagung Karlsruhe 2000; pp. 235-240. 278 Tab. 1: Field infection of wild alliums and hybrids with Allium cepa indexed with ELISA and TPIA Species ELISA (MS) TPIA (ST) A. cepa (female parent) OYDV+SLV or LYSV+SLV OYDV+SLV or SLV Male Parent Hybrid Male Parent Hybrid A. albidum LYSV or VF LYSV+SLV LYSV or VF LYSV+SLV or SLV or VF A. globosum SLV SLV SLV or VF SLV A. hymenorrhizum SLV or VF SLV or VF OYDV or VF SLV or VF A. lineare LYSV or VF SLV or VF LYSV or VF SLV or VF A. obliquum LYSV LYSV+SLV LYSV+SLV LYSV+SLV A. rubens VF SLV VF SLV A. saxatile VF SLV VF SLV A. senescens VF VF VF VF Abbreviations: ELISA – enzyme-linked immunosorbent assay, LYSV – leek yellow stripe virus, MS – mixed samples, OYDV – onion yellow dwarf virus, SLV – shallot latent virus, ST – single leaf test, TPIA – tissue print immunoassay, VF – virus free. The underlined viruses cause more or less serious disease symptoms.

Wild species as a source for introgression - the case of Allium<br />

FRITSCH, R.M. (2002): A short history of the taxonomic collection of genus Allium<br />

housed at the Institute of Plant Genetics and Crop Plant Research (IPK). In: L.<br />

MAGGIONI, J. KELLER and D. ASTLEY (compilers): European collections of vegetatively<br />

propagated Allium. Report of a Workshop, 21-22 May 2001, Gatersleben,<br />

Germany. International Plant Genetic Resources Institute, Rome, Italy. pp. 36-<br />

43.<br />

KELLER, E.R.J., I. SCHUBERT, J. FUCHS and A. MEISTER (1996): Interspecific crosses of<br />

onion with distant Allium species and characterization of the presumed hybrids<br />

by means of flow cytometry, karyotype analysis and genomic in situ hybridization.<br />

- Theor. Appl. Genet. 92, 417-424.<br />

PETERKA, H., H. BUDAHN and O. SCHRADER (1997): Interspecific hybrids between onion<br />

(Allium cepa L.) with S-cytoplasm and leek (A. ampeloprasum L.). - Theor.<br />

Appl. Genet. 94, 383-389.<br />

SCHULZ, H., H. KRÜGER, N. HERCHERT and E.R.J. KELLER (2000a) Vorkommen flüchtiger<br />

Sekundärmetaboliten in ausgewählten Allium-Wildtypen. - J. Appl. Bot. 74,<br />

119-121.<br />

SCHULZ, H., H. KRÜGER, N. HERCHERT, E.R.J. KELLER and M. KEUSGEN (2000b): Charakterisierung<br />

von Allium-Bastarden auf Basis der Aromaprofile. DGQ, XXXV.<br />

Vortragstagung Karlsruhe 2000; pp. 235-240.<br />

278<br />

Tab. 1: Field infection of wild alliums and hybrids with Allium cepa indexed<br />

with ELISA and TPIA<br />

Species ELISA (MS) TPIA (ST)<br />

A. cepa<br />

(female parent)<br />

OYDV+SLV or LYSV+SLV OYDV+SLV or SLV<br />

Male Parent Hybrid Male Parent Hybrid<br />

A. albidum LYSV or VF LYSV+SLV LYSV or VF LYSV+SLV<br />

or SLV<br />

or VF<br />

A. globosum SLV SLV SLV or VF SLV<br />

A. hymenorrhi<strong>zu</strong>m SLV or VF SLV or VF OYDV or VF SLV or VF<br />

A. lineare LYSV or VF SLV or VF LYSV or VF SLV or VF<br />

A. obliquum LYSV LYSV+SLV LYSV+SLV LYSV+SLV<br />

A. rubens VF SLV VF SLV<br />

A. saxatile VF SLV VF SLV<br />

A. senescens VF VF VF VF<br />

Abbreviations: ELISA – enzyme-linked immunosorbent assay, LYSV – leek yellow stripe virus,<br />

MS – mixed samples, OYDV – onion yellow dwarf virus, SLV – shallot latent virus, ST –<br />

single leaf test, TPIA – tissue print immunoassay, VF – virus free. The underlined viruses<br />

cause more or less serious disease symptoms.

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