Schriften zu Genetischen Ressourcen - Genres

Schriften zu Genetischen Ressourcen - Genres Schriften zu Genetischen Ressourcen - Genres

30.11.2012 Views

U. FREYTAG, G.H. BUCK-S ORLIN and B. SCHMIDT 2) PHA 6018: var. nanus ‘Michelite’: Virides with strings; qualified cultivar in France and the Netherlands. 3) PHA 6019: var. nanus ‘Redlands Greenleaf B’: Virides with strings. 4) PHA 6020: var. nanus ‘Dubbele Witte’: Virides with strings; pods 10-12 cm long, narrow to medium broad, flat oval, seed locations clearly marked, weakly to medium bending; pod whitish-green, seed very full, white to greyish-white, often with brown spots. 5) PHA 6021: var. vulgaris ‘Great Northern U.I. 31’. Virides with strings. 6) PHA 6022: var. nanus ‘Pinto 114’: Virides without strings. Cultivation – growing conditions Germination in bean takes places within a temperature frame of 10 to 37°C. Planting seeds outdoors can be done only when the medium daily temperature is 12-18°C (which is the case in Gatersleben at the beginning of May). Seedling emergence in the field requires, along with a soil temperature of at least 9°C, 7-14 days of 10-14°C air temperature. This ensures good seed maturity, which is particularly important for a genebank. The cultivation period (date of planting seeds to last harvest date) was between 106 and 159 days in Phaseolus vulgaris, depending on year and accession. Favourable for seed production is a dry climate; wind exposure, on the other hand, is unsuitable for bean cultivation, since, due to strong air movements, shoots are rubbed against each other and damaged, which often is followed by infections. Planting dates ranged between 5 May and 9 May, flowering dates between 18 June and 29 July, and harvest dates between 19 August and 12 October. Harvest was carried out on several occasions per accession. Assessment of diseases (Viruses, halo blight, alfalfa mosaic virus) was done during 1993 and 1994 in all accessions. Degree of infestation was assessed as “few halo spots” and “few viruses”. All accessions of the genebank were botanically identified in the field and assessed for the traits growth form, background pod colour, strings, standard pod colour, pod form, flowering date, flower colour, date of maturity, diseases, seed size, seed form, and seed colour. Additionally, agronomical traits were captured. These data were registered in a FoxPro database table. 259

Evaluation of pod, seed, and phenological traits of common beans Fig.1: Phaseolus vulgaris - Seeds from six accession about eight years Post-harvest treatment and seed storage in the cold depository Before depositing the seeds in the cold storage facility, they were dried in a separate room for 20 days at 20% humidity and a temperature of 20°C. Thus, a moisture content of 5-6% was achieved. Storage succeeded stepwise in cold chambers at 0°C and –15°C, from 2001 on at –15°C in 1-litre preserving jars with an addition of 160g silica gel per jar. Thousand grain weight Thousand Grain Weight was measured using the computer software WIN-TKG and stored directly on a PC. In doing so, the PC was connected via a serial interface to an electronic scale, which measured the weight of the seeds after counting using a seed counting machine. 260

U. FREYTAG, G.H. BUCK-S ORLIN and B. SCHMIDT<br />

2) PHA 6018: var. nanus ‘Michelite’: Virides with strings; qualified cultivar in France<br />

and the Netherlands.<br />

3) PHA 6019: var. nanus ‘Redlands Greenleaf B’: Virides with strings.<br />

4) PHA 6020: var. nanus ‘Dubbele Witte’: Virides with strings; pods 10-12 cm long,<br />

narrow to medium broad, flat oval, seed locations clearly marked, weakly to medium<br />

bending; pod whitish-green, seed very full, white to greyish-white, often with<br />

brown spots.<br />

5) PHA 6021: var. vulgaris ‘Great Northern U.I. 31’. Virides with strings.<br />

6) PHA 6022: var. nanus ‘Pinto 114’: Virides without strings.<br />

Cultivation – growing conditions<br />

Germination in bean takes places within a temperature frame of 10 to 37°C. Planting<br />

seeds outdoors can be done only when the medium daily temperature is 12-18°C<br />

(which is the case in Gatersleben at the beginning of May). Seedling emergence in<br />

the field requires, along with a soil temperature of at least 9°C, 7-14 days of 10-14°C<br />

air temperature. This ensures good seed maturity, which is particularly important for<br />

a genebank.<br />

The cultivation period (date of planting seeds to last harvest date) was between 106<br />

and 159 days in Phaseolus vulgaris, depending on year and accession. Favourable<br />

for seed production is a dry climate; wind exposure, on the other hand, is unsuitable<br />

for bean cultivation, since, due to strong air movements, shoots are rubbed against<br />

each other and damaged, which often is followed by infections.<br />

Planting dates ranged between 5 May and 9 May, flowering dates between 18 June<br />

and 29 July, and harvest dates between 19 August and 12 October. Harvest was carried<br />

out on several occasions per accession.<br />

Assessment of diseases (Viruses, halo blight, alfalfa mosaic virus) was done during<br />

1993 and 1994 in all accessions. Degree of infestation was assessed as “few halo<br />

spots” and “few viruses”.<br />

All accessions of the genebank were botanically identified in the field and assessed<br />

for the traits growth form, background pod colour, strings, standard pod colour, pod<br />

form, flowering date, flower colour, date of maturity, diseases, seed size, seed form,<br />

and seed colour. Additionally, agronomical traits were captured. These data were<br />

registered in a FoxPro database table.<br />

259

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