Schriften zu Genetischen Ressourcen - Genres

K.J. DEHMER
Towards a molecular taxonomy and localisation of origin in the
Solanum nigrum complex
K.J. DEHMER 1
Abstract
Molecular markers were employed within the taxonomically difficult Solanum nigrum
complex in order to characterise the genetic diversity present in the IPK genebank
accessions, to obtain new insights into the taxonomic subdivision of the complex, to
classify unknown accessions and to attempt an approximate localisation of the geographic
origin of accessions with unknown provenance.
After examining 69 entries from seven species, an initial species attribution of the material
seems already feasible by using only two SSR primer pairs. The respective results, in
some cases yielding hardly any differences at all between certain species towards a
differentiation on an interspecific level, were confirmed by AFLP analyses. In the latter,
unexpectedly high levels of infraspecific variation could be found as well, despite
geographically close origins. Furthermore, by the reflection of the provenance of the
material in several of the resulting clusters, clues on the currently unknown origin of some
of the genebank material might be obtained.
Overall, the two employed marker systems made it possible to classify at least 19 former
Solanum sp. accessions and to correct the classification of seven black nightshade
accessions.
Reference
DEHMER, K.J. (2001): Conclusions on the taxonomy of the Solanum nigrum complex by
molecular analyses of IPK germplasm accessions. In: R.G. VAN DEN BERG, G.W.M.
BARENDSE, G.M. VAN DER WEERDEN and C. MARIANI (Eds.): Solanaceae V: Advances
in taxonomy and utilization. Proceedings of the V th International Solanaceae
Conference, July 23-29 th 2000, Nijmegen. Nijmegen University Press, pp. 85-
1 Institute of Plant Genetics and Crop Plant Research (IPK)
Genebank Department, Molecular Markers Research Group
Corrensstr. 3
D-06466 Gatersleben, Germany
207