Schriften zu Genetischen Ressourcen - Genres

Proof of long-term stored potato germplasm by use of molecular markers
Proof of long-term stored potato germplasm by use of molecular
markers
O.Y. ANTONOVA 1 , L.I. KOSTINA 1 , T. GAVRILENKO 1 , K. SCHÜLER 2 and R. THIEME 3
Abstract
Specific SSRs were successfully used to generate fingerprints of cultivars which have
been long-term stored in different genebanks located at Groß Lüsewitz and St. Petersburg.
Using twelve pairs of SSR primers it was possible to distinguish all the
twelve cultivars used in the present study. By comparison of the banding patterns
identity in ten of twelve cultivars of both genebanks was proven. In the cultivars ‘Early
Rose’ and ‘Epicure’ differences in banding patterns were found for five and ten SSR
primer pairs, respectively. Conceivable reasons are discussed.
Introduction
The difficulty in confidently distinguishing cultivars is a problem facing potato breeders,
seed growers, micropropagators as well as germplasm curators. Proof of identity
of varieties plays an important role in the work with collections in genebanks. Clonal
identification in potato has been traditionally based upon morphological criteria.
However, limitations exist because this method is time-consuming. Therefore, numerous
attempts have been made to develop biochemical and DNA markers which
can be utilised in cultivar identification.
Our objective was to determine whether SSRs will generate fingerprints of cultivars
and can be used as an efficient and rapid technique for proof of identity of long-term
stored material.
1 N. I. Vavilov Institute of Plant Industry (VIR)
42, Bolshaya Morskaya str.
190000 St. Petersburg, Russia
2 IPK Genebank
External Branch “North”
D-18190 Groß Lüsewitz, Germany
3
Federal Centre for Breeding Research on Cultivated Plants
Rudolf-Schick-Platz 3
D-18190 Groß Lüsewitz, Germany
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