Antibody Drug Conjugates for Cancer Therapy
Antibody Drug Conjugates for Cancer Therapy Antibody Drug Conjugates for Cancer Therapy
How do we discover, define and validate MAb targets?•Abundance•Tumor specificityTaqman•Immunization•Screen AbProteinScreenReviewCloningReviewMAbs In Vivo Toxicity•Tumor specificity•Prevalence•Tissue specificity•Abundance•Transmembrane•Plasma membraneIn Situ•Cell type•Prevalence•Tumor specificityCell Line•Screen Ab•Internalization•Localization•In Vivo testing•Counter-screen• Immunization•Epitope•Affinity•Activity•FACS•IHC•Naked•Toxinconjugated•Clone Cynogene•Clone Ab•Chimeraproduction•conjugation
Target Screening- Large scale mRNA-based search for putative TM-containing proteins.Signal intensity (Ave. Diff. x 10 -3 )0 5 10 15 20 0 1 2 3 4 5adiposeadrenaladiposebreastTACSTD1/EpCAMCRC g1cervixCNScolorectalendometriumesophagushead&neckheartkidneyliverlungmuscleneurocrineovarypancreasprostateskinSm. intestinestomachtestisthyroidurinarywbc
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- Page 6 and 7: Naked antibodies are likely to be i
- Page 9 and 10: How many xenograft models should we
- Page 11 and 12: RelativeTenB2 expression in human p
- Page 13 and 14: Antibodies armed with auristatinsAN
- Page 15: MRP4 (multi drug resistance protein
- Page 18 and 19: Structure of the MUC16 antigen and
- Page 20 and 21: Endothelin B receptor: A target for
- Page 22 and 23: Melanocytes and EDNBR staining in n
- Page 24 and 25: Increased cell killing in vitro wit
- Page 26 and 27: ETBR Expression in Cell Lines versu
- Page 28 and 29: Efficacy/SafetyFour ways for armed
- Page 30 and 31: Antibody doses are normalized to dr
- Page 32 and 33: In Vivo Efficacy is Retained With T
- Page 34 and 35: Cumulative toxicity from long-lived
- Page 36: • GNE ADC Team- Susan Spencer- Ke
How do we discover, define and validate MAb targets?•Abundance•Tumor specificityTaqman•Immunization•Screen AbProteinScreenReviewCloningReviewMAbs In Vivo Toxicity•Tumor specificity•Prevalence•Tissue specificity•Abundance•Transmembrane•Plasma membraneIn Situ•Cell type•Prevalence•Tumor specificityCell Line•Screen Ab•Internalization•Localization•In Vivo testing•Counter-screen• Immunization•Epitope•Affinity•Activity•FACS•IHC•Naked•Toxinconjugated•Clone Cynogene•Clone Ab•Chimeraproduction•conjugation
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