Class III, Biological Safety Cabinets

Class III, Biological Safety CabinetsThe Premier Solution for High Containment LaboratoriesAirstream Class lll, Biological Safety Cabinet,Model AC3-4B_.

Main FeaturesExhaust air is double-filtered through high-qualityULPA filters (per IEST-RP-CC-001.3) with typicalefficiency of >99.999% for 0.1 to 0.3 micronparticles, better than HEPA filters.Exclusive dual exhaust filters provide >100,000times better protection than single-stage designs.Microprocessor-based Esco Sentinel Silver controlsystem provides visual / audible alarms for airflow.Magnehelic* pressure gauge is mounted in therear of the work zone for at-a-glance monitoringof work zone negative pressure.2Neoprene gauntlets are single piece leak-testedglove assemblies which guarantee maximumprotection.An integrated pass-through with interlockingdoors permits materials transfer without risk ofenvironmental contamination.Escoantimicrobial surface on allpainted surfaces minimizes contamination.An angled cabinet front ensures an ergonomicworking posture.Cabinet operates at negative pressure relative tothe laboratory in order to prevent migration ofpathogenic materials out of the work area.*Registered trademark of Dwyer Instruments, Inc.Airstream Class III Biological Safety Cabinet.Model AC3-4B_.Biological Safety Cabinets • Class III Biological Safety Cabinets

capability than conventional HEPAfilters that have a typical efficiency of>99.99% for 0.3 micron particles.• Modern separator-less mini-pleat filterconstruction maximizes the filter surfacearea to extend filter life and eliminatespossible filter media damage by thinand sharp aluminum separators used inconventional HEPA filter construction.• The filter frame and media is constructedin accordance with EN1822 requirementsfor fire retardant properties• The supply ULPA filter provides ISOClass 3 (per ISO14644.1) clean air to thework surface in a gentle vertical flow forproduct protection.• The dual exhaust ULPA filters trapaerosols generated in the work areabefore air is exhausted to the roomproviding personnel and environmentalprotection.• The exhaust filter media is protectedfrom mechanical damage by anintegrated metal screen guard, which isabsent from conventional HEPA filters.Neoprene synthetic arm-length gauntletsare flame and abrasion resistant. Single piece,leak-tested assembly guarantees maximumprotection. Black color allows the detection ofleaks by visual means.Containment and ProtectionThe Esco Airstream Class III BiologicalSafety Cabinet provides the industry’s bestprotection for high-hazard applications.• Complete welded construction ensuresan air-tight carcass for absolute containment,operator and environmentalsafety.• Dual exhaust ULPA filters deliver superiorprotection - the combined typicalefficiency of the two exhaust filtersin series allows only 1 particle out of10 billion (of 0.1 - 0.3 microns size) topenetrate. Filtration efficiency is evenhigher for particles larger than 0.3 micronsor smaller than 0.1 microns.• Inflow room air is ULPA filtered to establishproduct protection; unfilteredroom air does not enter the work zone,preventing product contamination.• Integrated pass-through with interlockingdoors allows materials andequipment to be easily passed intothe main chamber without the risk ofenvironmental contamination.• Magnehelic* pressure gauge mountedin the rear wall of the work zone enablescontinuous monitoring of workzone negative pressure.Operator, Product and Environmental ProtectionThe Esco Airstream Class III Biological Safety Cabinet(AC3) offers the highest possible level of containmentand protection. The cabinet's airtight seal and advancedULPA filtered laminar airflow provides product,operator and environmental protection and is suitablefor use with agents assigned to Biosafety Levels 1,2, 3 and 4. The AC3 is frequently used for workinvolving the deadliest biohazards, bacteria, viruses andmicroorganisms.Mini-pleat Separatorless Filter (left) vs.Conventional Aluminum Separator Filter (right)Esco cabinets use Swedish Camfil Farr ® mini-pleatfilters without aluminum separators to increase filterefficiency, minimize the chance of leakage, and toprolong filter life. Filters include a lightweightaluminum frame for structural stability andelimination of swelling common to conventionalwood frames.Blower SystemThe Airstream blower system is designedfor high performance operation, maximumenergy efficiency and minimalmaintenance.• Dual fan design ensures cabinet containmentand operator safety in the event ofa single fan failure.• The permanently lubricated direct-driveexternal rotor motor / blower reducesoperating costs.• The external rotor motor design allowsfor optimum cooling of the motorduring extended operations and extendsthe motor bearing life.• An integral blower hour meter tracksoperating life and aids in predictivemaintenance planning.Sentinel TM Microprocessor Control,Alarm, Monitoring SystemThe Esco Sentinel microprocessor-basedcontrol system supervises operation of allcabinet functions.3*Registered trademark of Dwyer Instruments, IncIntegrated Filtration SystemA combination of a supply ULPA filter anddual exhaust ULPA filters give the AC3cabinet a fully integrated performanceenvelope for product, operator andenvironmental protection.• ULPA filters (per IEST-RP-CC001.3),are tested to a typical efficiency of>99.999% for 0.1 to 0.3 micronparticles; these provide better filtrationEsco ULPA Filter EfficiencyTypical PenetrationIndependent supply and exhaustfilters provide 99.999% typicalefficiency for particle sizes of 0.1to 0.3 microns. Airstream Seriesfilters meet the IEST-RP-CC001.3recommended practice for ULPAperformance (USA), and EN 1822for H14 performance (EU).

Cabinet Filtration SystemDynamic air barrier, inflow and forward-directed downflow air convergeAmbient air is pulled into the cabinetthrough a disposable pre-filter, trappinglarger particles and prolonging the lifeof main filter. Air is then passed throughthe downflow ULPA filter into the workzone as a vertical laminar flow air stream,bathing the work surface in clean air.The uniform non-turbulent air streamprotects against cross contaminationwithin and throughout the work area.Near the work surface, the downflowair stream splits and exits the chamberthrough perforations around theperimeter of the work zone.ULPA-filtered airUnfiltered / potentially contaminated airRoom air / Inflow airAir from work zone passes through thefirst ULPA exhaust filter below the worksurface, before traveling through internalducting and exhausting to the laboratoryenvironment after passing through thesecond ULPA exhaust filter located abovethe cabinet's main chamber.Biosafety Cabinets For Air Quality For Filtration For Electrical SafetyStandardsComplianceEN 12469, EuropeISO 14644.1 Class 3, WorldwideIEST-G-CC1001, USAIEST-G-CC1002, USAIEST-RP-CC034.1, WorldwideIEST-RP-CC007.1, WorldwideIEST-RP-CC001.3, WorldwideEN 1822 (H14), EuropeIEC 61010-1, WorldwideEN 61010-1, EuropeUL 61010-1, USACAN/CSA-22.2, No. 61010-14• The control panel is located in thecenter of the cabinet and angled downfor easy access by the operator.• Cabinet airflow is monitored continuouslyand displayed on a bright, easyto-readLCD display.• An integrated, temperature-compensatedtrue airflow velocity sensorprovides an accurate airflow reading despiteroom temperature fluctuation.• All electronic parts are contained insidea plug-and-play module that permitseasy exchange if required.• Microprocessor software updates areavailable from Esco for download viathe Internet.Sentinel functions are factory set to defaultto ON or OFF, depending on worldwidedestination and local preferences.Default settings can be user activatedthrough the touchpad data entry access.• Automatic start-up sequence will preparethe cabinet for normal operationand advise when safe conditions areestablished.• An administrator controlled PIN (personalidentification number) can be setto restrict access to main menu.• The airflow alarm can be activated ordeactivated depending on user preferenceand nature of the work.Consult your Esco Operating Manualor contact your Sales Representativefor information on user-preferenceprogramming capabilities built into theSentinel microprocessor platform.Cabinet ConstructionRobust construction and enhanced safetyfeatures qualify the cabinet for the mostdemanding laboratory applications. Thecabinet is fully assembled and ready toinstall and operate when shipped.• All stainless steel work surface is easyto clean.• Optional electrical service fittings arelocated for easier access.8"Glove Sizes• External surfaces are coated with EscoIsocide antimicrobial coating to protectagainst surface contamination andinhibit bacterial growth. Isocide eliminates99.9% of surface bacteria within24 hours of exposure.• Industrial grade support frame constructedof electrogalvanised steel withan abrasion resistant finish.Comfortable Ergonomic DesignThe AC3 cabinet is engineered for comfort,utility value and safety.• The angled front improves reach intothe work area and reduces glare fromthe glass surface.• The instant-start 5000k fluorescentlamp operates on an electronic ballastto reduce heat, improve comfort andconserve energy.To find out your glove size, measure (in inches) around yourhand with a tape measure at the place indicated on the diagram.This measurement, in inches, is the closest to your glove size,i.e. 8” is equal to a glove size of 8.You should use your dominant hand, the right if you are righthanded,and the left if you are left-handed.Standard glove sizes available are 7, 8 (standard size) and 9.Specify when ordering.Biological Safety Cabinets • Class III Biological Safety Cabinets

Touchpad data entry buttons permitcontrol settings and access to diagnostics,default settings and hierarchical menus.Color coded indicator lamps display green forprimary function (fan operation); blue for secondaryfunction (fluorescent lights and electrical outlet);and orange for caution (UV lamp ON).Programmable automatic UV lighttimer simplifies operation, enhancescontamination control, extends UVlamp life and saves energy.Digital read-out with alpha-numeric display indicatesall input, status and alarm functions.All functions can be user activated through touch-padprogramming access; see Operations Manual.Sentinel Microprocessor Control System, ProgrammableWhen programmed ON • the start-up sequence confirms status with Air Safe and local time display.• the Personal Identification Number (PIN) access restricts unauthorized adjustments.• an airflow alarm warns of deviations from normal velocities.• The lamp delivers uniform lighting tothe work surface for greater comfort,reduced glare and improved productivity(see Technical Specifications).• Neoprene synthetic arm-length gauntletsare flame and abrasion resistant.Single piece, leak-tested assembly guaranteesmaximum protection. Black colorallows the detection of leaks by visualmeans.• Glove ports are designed for easy andsafe glove changing.Electrical Safety and CertificationAll components meet or exceed applicablesafety requirements.• Each cabinet is individually factorytested for electrical safety.• Documentation specific to each cabinetserial number is maintained on file.• All electrical components are UL-listedor recognized.Contact Esco or your Sales Representativefor site preparation information (seeElectrical Specifications).WarrantyThe AC3 is warranted for 3 years excludingconsumable parts and accessories.• Each cabinet is shipped with a comprehensiveUser’s Manual completewith a report documenting all testprocedures.• Additional IQ/OQ documentation isavailable upon request.Contact your local Sales Representative forspecific warranty details or documentationrequests.Accessories and OptionsEsco offers a variety of options and accessoriesto meet local applications. ContactEsco or your local Sales Representative forordering information.Electrical Outlets and Utility Fixtures• Electrical outlet, ground fault,North America• Electrical outlet, Europe / WorldwideCabinet Accessories• Germicidal UV lamp- Controlled by automatic UV lamptimer through Sentinel TM microprocessorcontrol panel5Antimicrobial Powder-CoatingBacterial Population (10 fold)8642All exterior painted surfaces are powder-coatedwith Esco Isocide, an antimicrobial inhibitor tominimize contamination. Isocide is integrated intothe coating substrate and cannot wash out ordiminish by repeated cleaning. Performance resultsare available upon request. Contact Esco or yourEsco Sales Representative for details.0 4 hours 8 hours 12 hours 16 hours 20 hours 24 hoursContact Time in Hours

General Specifications, Airstream Class lll, Biological Safety CabinetsNote to customer: Insert electrical voltage number into last model number digits _ when orderingModel AC3-4B__ AC3-5B__ AC3-6B__Nominal Size 1.2 meters (4') 1.5 meters (5') 1.8 meters (6')External Dimensions (W x D x H)1665 x 850 x 2250 mm65.6" x 33.5" x 88.6"1970 x 850 x 2250 mm77.6" x 33.5" x 88.6"2275 x 850 x 2250 mm89.6" x 33.5" x 88.6"Internal Work Area, Dimensions(W x D x H)1130 x 560 x 650 mm44.5" x 22.0" x 25.6"1435 x 560 x 650 mm56.5" x 22.0" x 25.6"1740 x 560 x 650 mm68.5" x 22.0" x 25.6"Internal Work Area, Space 0.63 m 2 (6.8 sq.ft) 0.80 m 2 (8.6 sq.ft) 0.97 m 2 (10.4 sq.ft)Number of Gloves Ports 2 ports 4 ports 4 portsGlove Type & Sizes AvailableNeoprene TM polychloroprene synthetic rubber gauntlets.Available in sizes 7, 8 (standard size) and 9Initial Airflow Volume 603 m 3 /h ( 355 cfm) 756 m 3 /h ( 445 cfm) 902 m 3 /h (531 cfm)Negative Work Zone Pressure-275 Pa (-1.1" Wg)6Pre-FilterULPA Filter Typical Efficiency(Downflow, 1st Exhaust, 2nd Exhaust)SoundEmission(Typical)*Disposable and non-washable polyester fibers with 85% arrestance / EU3 ratedTypical: 99.999% at 0.1 to 0.3 μm and MPPSNSF 49 186 foot candles)1.5 mm (0.06") 16 gauge electro-galvanized steel with white oven-bakedepoxy-polyester Isocide antimicrobial powder coated finish1.2 mm (0.05") 18 gauge stainless steel, type 304, with 4B finish220-240V, AC, 50Hz, 1 AC3-4B1 AC3-5B1 AC3-6B1Cabinet Power/ Amp 520 W/ 3 A 525 W/ 3 A 550 W/ 3 AOutlet Maximum Amp 5 A 5 A 5 AFull Load Amps 8 A 8 A 8 ABTU/ Hr 1061 1071 1122110-120V, AC, 60Hz, 1 AC3-4B2 AC3-5B2 AC3-6B2Cabinet Power/ Amp 1000 W/ 10 A 1200 W/ 11.5 A 1200 W/ 11.5 AOutlet Maximum Amp 5 A 5 A 5 AFull Load Amps 15 A 16.5 A 16.5 ABTU/ Hr 2040 2448 2448Net Weight 498 kg (1096 lbs) 598 kg (1316 lbs) 676 kg (1487 lbs)Shipping Weight 606 kg (1336 lbs) 697 kg (1536 lbs) 788 kg (1737 lbs)Shipping Dimensions,Maximum (W x D x H)1960 x 1320 x 2650 mm77.2" x 51.9" x 104.3"2265 x 1320 x 2750 mm89.2" x 51.9" x 108.3"2550 x 1320 x 2750 mm100.4" x 51.9" x 108.3"Shipping Volume, Maximum 6.86 m 3 (242 cu.ft.) 8.1 m 3 ( 286 cu.ft.) 8.92 m 3 (315 cu.ft.)* Noise reading in open field condition/ anechoic chamber.** Additional voltages may be available; contact Esco for ordering information.(Cont.)- Emission of 253.7 nanometers formost efficient decontamination• Ergonomic lab chair- Laboratory grade construction,meets Class 100 cleanliness; alcoholresistant PVC materials- Adjustable height 395-490 mm (15.6"-19.3")• Ergonomic foot rest- Angled, helps maintain proper posture- Adjustable height- Anti-skid coating, chemicalresistant finish• Formalin vaporizerThe Airtsream Class lll Biological Safety Cabinetis available in all stainless steel construction forpharmaceutical grade applications, contact Escoor your Sales Representative for more details.Biological Safety Cabinets • Class III Biological Safety Cabinets

340 mm13.4"650 mm (25.6")900 mm (35.4")2250 mm (100.4")250 mm9.8"Airstream Model AC3 Class lll Biological Safety Cabinet Technical SpecificationsModel AC3-4B_Model AC3-5B_15151112132121321414400 mm15.7"400 mm15.7"450 mm17.7"400 mm15.7"330 mm13.0"400 mm15.7"1340 mm (52.8")1665 mm (65.5")1645 mm (64.8")1970 mm (77.6")Model AC3-6B_Common Side View250 mm9.8"71531041112131256290 mm (11.4")147400 mm15.7"8450 mm17.7"400 mm15.7"450 mm17.7"91950 mm (76.8")2275 mm (89.6")850 mm (33.5")1 Esco Sentinel microprocessor control system2. Pass box3. Pre- filter4. Electrical & electronics panel5. ULPA filter (downflow)6. Fluorescent light7. Glove ports8. First exhaust ULPA filter9. First exhaust blower10. Second exhaust ULPA filter11. Second exhaust blower12. UV lamps (optional)13. Pressure gauge14. Work zone back wall: optional electrical outletRetrofit Kit provisions (2 outlets in work zoneone on each side)15. Exhaust collar (optional)

8Biological Safety ProductsCleanroom ProductsContainment / Pharma ProductsDuctless Fume Hoods / Carbon FiltrationGeneral Purpose Scientific EquipmentIndustrial Lab EquipmentIn-Vitro Fertilization ProductsPCR ProductsPharmacy ProductsLab Animal Research ProductsLab Thermostatics ProductsLab Ventilation / Chemical Fume Products / Lab FurniturePowder Handling ProductsSince 1978, Esco has emerged as a leader in the development of controlled environment, laboratory and cleanroomequipment solutions. Products sold in more than 100 countries include biological safety cabinets, fume hoods, ductlessfume hoods, laminar flow clean benches, animal containment workstations, cytotoxic cabinets, hospital pharmacy isolators,and PCR cabinets and instrumentation. With the most extensive product line in the industry, Esco has passed more tests,in more languages, for more certifications, throughout more countries than any biosafety cabinet manufacturer in theworld. Esco remains dedicated to delivering innovative solutions for the clinical, life science, research and industriallaboratory community. www.escoglobal.com.Biological Safety Cabinets and Laminar Flow • Laboratory Fume Hoods • Laboratory OvensLaboratory Incubators • PCR Thermal Cyclers • Microplate Shaker/Incubators • Ultraflow FreezersEsco Technologies, Inc. • 2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USAToll-Free USA and Canada 877-479-ESCO • Tel 215-441-9661 • Fax 215-441-9660us.escoglobal.com • usa@escoglobal.comEsco Micro Pte. Ltd. • 21 Changi South Street 1 • Singapore 486 777Tel +65 6542 0833 • Fax +65 6542 6920 • mail@escoglobal.comwww.escoglobal.comEsco Global Offices | Kuala Lumpur, Malaysia | Leiden, The Netherlands | Manama, BahrainMumbai, India | Philadelphia, USA | Salisbury, UK | Shanghai, China | Singapore©2009 Esco Micro Pte. Ltd. Specifications Subject to Change. ES1268F_V2_3K_06/09escoglobal.comLIVE SUPPORTEsco Micro Pte LtdCert. No: 651333PT Esco Bintan IndonesiaCert. No: 651333/1