full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1163-1172 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1168Table 1. Details <strong>of</strong> the plant samples used in the study.S No. Name <strong>of</strong> the species Place <strong>of</strong> collection Accession No.1 C. fenestratum Bangalore market, KA L/06/09/0312 Kanyakumari, TN L/06/12/0153 Pune, MH L/07/02/0334 Bangalore market, KA L/07/04/0155 Nadavayal forest, Wayanad, KL L/07/08/0106 B. aristata Potter’s hill, Shimla, HP L/08/09/0127 Shillaru, Shimla, HP L/08/10/0088 Potter’s Hills, Shimla, HP L/08/10/0119 B.asiatica Mulshi, Mussoorie, HP L/07/12/00110 Saharan-Nahar Road, HP L/08/09/01311 Saharan-Nahar road, HP L/08/09/01612 B.lycium Karol, Manikaran, HP L/06/06/03713 Shillaru, Kangra, HP L/08/10/00914 Chail, Solan, HP L/08/10/01015 M. umbellata Kannimarsholai, Tiruchi, TN L/07/04/00116 Sirumalai hills, Dindigul, TN L/07/07/05317 Mavanatham, Erode, TN L/09/03/009Table 2. Details <strong>of</strong> the primers used for amplifying the ITS1 <strong>and</strong> ITS2 regions <strong>of</strong> C. fenestratumPrimer detailsRegion Direction Name <strong>of</strong> Primer sequence (5’ 3’) Size <strong>of</strong> the GenBankthe primer amplicon accession No.ITS1 Forward ITS 1 TCCGTAGGTGAACCTGCGG 245 bp GQ496588Reverse ITS 2 GCTGCGTTCTTCATCGATGCITS2 Forward ITS 3 GCATCGATGAAGAACGCAGC 386 bp GQ496589Reverse ITS 4 TCCTCCGCTTATTGATATGCTable 3. The homology score in the ITS regions <strong>of</strong> C. fenestratum with its adulterant speciesAdulterant Species GenBank Homology %accession no. ITS1 ITS2B. aristata GQ259132 43.87 37.03B. asiatica GQ259133 52.55 34.44B. lycium GQ259134 51.53 35.18M. umbellata AY514063 52.04 56.66Balasubramani <strong>and</strong> Venkatasubramanian