full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1157-1162 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1161growth <strong>of</strong> the larvae. 2% gly twice a day treatmentstarted from the 2 nd day <strong>of</strong> V th instar larvae hasfound to have maximum silk gl<strong>and</strong> growth. Onthe other h<strong>and</strong>, high growth <strong>of</strong> the larvae wasfound to have higher cocoon weight as found incase <strong>of</strong> 2% gly once a day treatment from the 1 stday treatment <strong>of</strong> the V th instar larvae. However,the higher silk gl<strong>and</strong> growth with 2% gly twice aday has found to have maximum shell weight.Therefore, it contributes to the low pupa growth.In contrast, maximum pupa growth was observedwith minimal larvae <strong>and</strong> gl<strong>and</strong> growth with 2%gly once a day treatment <strong>of</strong> the 1 st day <strong>of</strong> V thinstars larvae. But in such case maximum cocoonweight was noticed. Therefore, it is clear thatsilk gl<strong>and</strong> <strong>and</strong> larval growth has not positiverelation with pupa weight <strong>and</strong> shell weight. It ishere to mention that both the pupa <strong>and</strong> shell onthe raw products <strong>of</strong> the sericulture have differentapplications. Therefore, the exact biochemical orphysiological role for the L-gly supplementationwith different concentrations from the day 1 or 2<strong>of</strong> the V th instar <strong>of</strong> B. mori larvae is a matter <strong>of</strong>future investigation.ConclusionIt can be concluded form the presentstudy that the maximum silk gl<strong>and</strong> protein orcocoon or shell production with 2% gly smearedmulberry leaves with twice a day supplementationfrom the 2 nd day or 2% gly once a day or 2% glytwice a day from the 2 nd day <strong>of</strong> the V th instar,respectively, are required. The abovephenomenon clearly indicates that gly havespecific effect on the growth <strong>of</strong> the larvae, gl<strong>and</strong>,pupa <strong>and</strong> cocoon production in the Nistari variety<strong>of</strong> the silk worm B. Mori.AcknowledgementsThis study was a project work undertaken by BRP for the partial fulfillment <strong>of</strong> MSc inZoology at Utkal University. BRP is extremelygrateful to the University Grants Commission,New Delhi for providing a fellowship underRFSMS scheme (F.4-3/2006 (XI plan/BSR/7-173/2007(BSR). The authors are highly thankful tothe Head, Department <strong>of</strong> Zoology for providingthe laboratory facilities.References1. Davis G. R. F. (1978). Growth response <strong>of</strong>larvae <strong>of</strong> Tenebrio molitor to concentrations<strong>of</strong> dietary amino acids. Journal <strong>of</strong> StoredProducts Research, 14:69-71.2. Babu, V. P., Gowda, B. L. V., Govindan, R.<strong>and</strong> Gowda, M. (1999). Effect <strong>of</strong> L-Glycineon Growth <strong>of</strong> Silkworm Bombyx mori L.Entomon, 25:335-339.3. Eid, M. A. A., El-Nakkady, A. N. <strong>and</strong> Salem,M. A. (1988). Effects <strong>of</strong> supplementaryamino acids on silk secretion by larvae <strong>of</strong>Philosamia ricini (Boisd.). Indian Journal<strong>of</strong> Sericulture, 28: 224-232.4. Etebari, K., Ebadi, R. <strong>and</strong> Matindoost, L.(2007). Physiological Changes <strong>of</strong> Silkworm(Bombyx mori L.) Larvae Fed on MulberryLaves Supplemented with Nitrogenous.Compounds. Journal <strong>of</strong> the EntomologicalResearch Society, 9:1-15.5. Rao, D. M. R., Kod<strong>and</strong>aramaiah, J., Reddy,M. P., Katiyar, R. S. <strong>and</strong> Rahmathulla, V.K. (2007). Effect <strong>of</strong> VAM fungi <strong>and</strong>bacterial bi<strong>of</strong>ertilizers on mulberry leafquality <strong>and</strong> silkworm cocoon charactersunder semiarid conditions. Caspian Journal<strong>of</strong> Environmental Science, 5:111-117.6. Raman, C., Manohar, S. L., Xavier, N. <strong>and</strong>Krishnan, M. (2007). Expression <strong>of</strong> silk genein response to P-soyatose (hydrolyzed soybean protein) supplementation in the fifthinstar male larvae <strong>of</strong> Bombyx mori. Journal<strong>of</strong> Cell Molecular Biology, 6:163-174.Effect <strong>of</strong> glycine on growth <strong>of</strong> Bombyx mori