full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1134-1148 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1146Table 5. List <strong>of</strong> protiens (manily enzymes) having active role in biosynthetic <strong>and</strong> metabolic pathways <strong>of</strong> Bacillus cereusS.No. Gene ID Protein name Role EC Number<strong>of</strong> promising drug target protein in each species.Finally 82 genes in Listeria monocytogenes <strong>and</strong>99 genes in Bacillus cereus were found whichcan be used as promising drug targets. The targetsgenes identified are involved in regulator protein,membrane proteins <strong>and</strong> hypothetical proteins <strong>of</strong>unknown function <strong>of</strong> bacterial species. No similarprotein is functional in human. Further KEGGpathway prediction shows that 20 proteins inBacillus cereus <strong>and</strong> 9 proteins in Listeriamonocytogenes were found to have active rolein biosynthetic <strong>and</strong> metabolic pathways (Table 4<strong>and</strong> Table 5). The data for total number <strong>of</strong> genesequence following non human homologoussequences <strong>and</strong> essential genes are shown in Table 1.Recent publication <strong>of</strong> genome sequences<strong>of</strong> many organisms <strong>and</strong> several newbioinformatics tools have facilitated identification<strong>of</strong> antimicrobial drug targets in pharmacogenomics.In recent study, RecA was found animportant factor in DNA repair <strong>and</strong> the activator<strong>of</strong> the SOS response that contributes to theresistance against acid <strong>and</strong> bile <strong>and</strong> to the ability<strong>of</strong> L. monocytogenes to adhere <strong>and</strong> invade humanintestine epithelial cells (15). Present study revealswith in silico analysis <strong>of</strong> essential genes as apreliminary step to screen through genome <strong>of</strong>these pathogens. We found some enzyme proteinsactively involved in various processes like DNAreplication, recombination, repairing etc. thusthese enzyme/proteins can be used to deactivateRecA to inhibit further growth <strong>of</strong> L.monocytogenes. On average very less number<strong>of</strong> genes was annoted as essential genes so toallow for experimental analysis, leading to asystematic strategy in designing novelantimicrobial active compounds. These genes are<strong>of</strong> interest for further characterization tounderst<strong>and</strong> role <strong>of</strong> these gene in survival <strong>of</strong>species, as multi-drug target as they are involvedin regulator protein, membrane proteins involvedin biosynthetic <strong>and</strong> metabolic pathways <strong>and</strong>hypothetical proteins <strong>of</strong> unknown function <strong>of</strong>bacterial species. The compounds active on thesegenes are expected to be more effective for lethalaction on bacteria without affecting host body.The long term goal <strong>of</strong> the study is to analyse severalpathogens genome <strong>and</strong> prepare an extensivedatabase <strong>of</strong> essential drug target proteins databseas scientific resource.Genome analysis <strong>of</strong> selected foodborne pathogens