full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and PharmacyVol. 5 (2) 1134-1148 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1146Table 5. List of protiens (manily enzymes) having active role in biosynthetic and metabolic pathways of Bacillus cereusS.No. Gene ID Protein name Role EC Numberof promising drug target protein in each species.Finally 82 genes in Listeria monocytogenes and99 genes in Bacillus cereus were found whichcan be used as promising drug targets. The targetsgenes identified are involved in regulator protein,membrane proteins and hypothetical proteins ofunknown function of bacterial species. No similarprotein is functional in human. Further KEGGpathway prediction shows that 20 proteins inBacillus cereus and 9 proteins in Listeriamonocytogenes were found to have active rolein biosynthetic and metabolic pathways (Table 4and Table 5). The data for total number of genesequence following non human homologoussequences and essential genes are shown in Table 1.Recent publication of genome sequencesof many organisms and several newbioinformatics tools have facilitated identificationof antimicrobial drug targets in pharmacogenomics.In recent study, RecA was found animportant factor in DNA repair and the activatorof the SOS response that contributes to theresistance against acid and bile and to the abilityof L. monocytogenes to adhere and invade humanintestine epithelial cells (15). Present study revealswith in silico analysis of essential genes as apreliminary step to screen through genome ofthese pathogens. We found some enzyme proteinsactively involved in various processes like DNAreplication, recombination, repairing etc. thusthese enzyme/proteins can be used to deactivateRecA to inhibit further growth of L.monocytogenes. On average very less numberof genes was annoted as essential genes so toallow for experimental analysis, leading to asystematic strategy in designing novelantimicrobial active compounds. These genes areof interest for further characterization tounderstand role of these gene in survival ofspecies, as multi-drug target as they are involvedin regulator protein, membrane proteins involvedin biosynthetic and metabolic pathways andhypothetical proteins of unknown function ofbacterial species. The compounds active on thesegenes are expected to be more effective for lethalaction on bacteria without affecting host body.The long term goal of the study is to analyse severalpathogens genome and prepare an extensivedatabase of essential drug target proteins databseas scientific resource.Genome analysis of selected foodborne pathogens