full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and PharmacyVol. 5 (2) 1110-1122 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1115Table 1. Composition of Carvedilol loaded nano emulsions.Volume of oil phase (ml) Quantity of Volume ofCode surfactant (mg) aqueous phase (ml)Sesame oil Olive oil Brij 97 Tween 80C1 100 - 150 - 10C2 150 - 150 - 10C3 200 - 150 - 10C4 150 - 125 - 10C5 150 - 175 - 10C6 100 - - 150 10C7 150 - 150 10C8 200 - - 150 10C9 150 - - 125 10C10 150 - - 175 10C11 - 100 150 - 10C12 - 150 150 - 10C13 - 200 150 - 10C14 - 150 125 - 10C15 - 150 175 - 10C16 - 100 - 150 10C17 - 150 - 150 10C18 - 200 - 150 10C19 - 150 - 125 10C20 - 150 - 175 10zeta size (nm)volume of oil phaseFig.1. Effect of oil phase volume on particle size ofnano emulsions a C, S, O, B and T represents thecarvedilol, Sesame oil, Olive oil, Brij 97 and Tween 80respectively.1% tween 80 for the formulations (C14, C4, C7and C17) that showed maximum drug loading andnarrow globule size and narrow PDI . The invitrodrug release profiles for the formulationsC14, C4, C7 and C17 were shown in Fig-2A and2B. The cumulative amount released was63.68±0.8%, 31.8±3.0%, 27.56±9.2%, and38.04±5.9% in formulations C14, C4, C7 and C17respectively. Plots of log % drug remaining againsttime were linear which indicates that the rate ofdrug release follows first order kinetics.X-ray diffraction studies: X-ray diffractionstudies were carried out to reveal the crystallinemodification of the drug during preparation of nanoKoteswari Poluri et al