Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1104-1109 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1107much use <strong>of</strong> IC 50to pIC 50value conversion. Wecan convert IC 50value to pIC 50value which isdeveloped by using JavaScript <strong>and</strong> HTML, <strong>and</strong>is placed in website <strong>of</strong> Sanjeevslab. The scriptfor the pIC 50calculator has been developed forprediction <strong>of</strong> pIC 50value with IC 50value units <strong>of</strong>nanomolar <strong>and</strong> micromolar. Here (Fig. 2) thebackground information <strong>of</strong> the program showingthe mathematical calculation <strong>of</strong> pIC 50value. TheIC 50value <strong>of</strong> compound staurosporine, which isknown to have anti-fungal activity <strong>of</strong> 2.7nM (16),is given in pIC 50calculator. Here, the 2.7 as inputvalue is get through the ‘document.getElementById (‘ic’).value = “”;’ declaring thisinput value as var a for the calculation <strong>of</strong> pIC 50value. During this calculation the log value <strong>of</strong> 2.7to the base10 is calculated as 0.431363764, butthe IC 50value is in nanomolar. There is againneed <strong>of</strong> conversion with respect to the nanomolar,so it is subtracted with 9. Finally the logarithmicvalue is converted in nanomolar <strong>and</strong> finally thepIC 50value is calculated as 8.56863 (Fig. 2). Inthis algorithm development <strong>of</strong> pIC 50calculatorwith combined approach <strong>of</strong> JavaScript <strong>and</strong> HTMLhaving a potential to find out accurate pIC 50value.This pIC 50value calculator will be helpful forquick validation <strong>of</strong> next generation drug discovery<strong>and</strong> development against drug target. The in vitroFig.2 Representing the output IC 50value to pIC 50valuesthrough pIC 50Calculator.biological activity data reported in form <strong>of</strong> IC 50value for inhibition <strong>of</strong> CDK2 by two series <strong>of</strong>aminothiazole derivatives was used for thisvalidation. According to work by Singh et al.,(19)pIC 50values <strong>of</strong> Aminothiazole Derivatives fromIC 50values was manually calculated <strong>and</strong> in thispaper initial IC 50data obtained from thesynthesized Aminothiazole Derivatives by Misraet al. (20) (Table 1. & Fig 3).Ch<strong>and</strong>rabose Selvaraj et al
Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1104-1109 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1108Fig. 3 Representing the resemblance <strong>of</strong> calculatedpIC 50value manually <strong>and</strong> with pIC 50calculatorThese values are analyzed for calculation<strong>and</strong> accuracy <strong>of</strong> the algorithm are tabulated. Thevalues published as pIC 50value are accurate <strong>and</strong>clear decimal values are obtained; both the valuesare differentiated in bar diagram, which showsthat developed algorithm is accurate. (Table 1)<strong>and</strong> contains 17 synthesized lig<strong>and</strong> compound,represented in Singh et al. The bar diagramenclosed, represents the correctness <strong>and</strong>similarities in values which is predicted by thepIC50 calculator <strong>and</strong> manually. Here the valuesare more accurate due to the programmingalgorithm <strong>and</strong> gives the value up to 10 decimalpoints. Bar diagram shows correlation withavailable values without much deviations, whichshows predicted pIC 50values are accurate <strong>and</strong>can be used further for other calculation <strong>and</strong>prediction in computer aided drug designing. Thus,the algorithm is much useful for drug designingcommunity <strong>and</strong> user can minimize their time forcalculations, especially those who are workingwith large data set related to pharmacoinformatics<strong>and</strong> chemoinformatics.AcknowledgementsAuthors are kindly acknowledging 110mbserver for hosting the webpage <strong>and</strong> pIC 50calculator<strong>and</strong> one author Ch<strong>and</strong>rabose Selvaraj thank<strong>full</strong>yacknowledging Alagappa University for researchfellowship.References1. Tropsha, A <strong>and</strong> Golbraikh, A., (2007)Predictive QSAR modeling workflow, modelapplicability domains, <strong>and</strong> virtualscreening.Curr Pharm Des. 13:3494-504.2. Dixon, S.L., Smondyrev, A.M., Knoll, E.H.,Rao, S.N., Shaw, D.E <strong>and</strong> Friesner, R.A.,(2006) PHASE: a new engine forpharmacophore perception, 3D QSAR modeldevelopment, <strong>and</strong> 3D database screening: 1.Methodology <strong>and</strong> preliminary results. JComput Aided Mol Des. 20:647-71.3. Lyles, R. H., Poindexter, C., Evans, A.,Brown, M. <strong>and</strong> Carlton R. Cooper. (2008).Nonlinear model-based estimates <strong>of</strong> IC 50forstudies involving continuous therapeuticdose–response data. Contemporary ClinicalTrials, 29: 878-886.4. Yung-Chi, C. <strong>and</strong> Prus<strong>of</strong>f, W. H. (1973).Relationship between the inhibition constant(KI) <strong>and</strong> the concentration <strong>of</strong> inhibitor whichcauses 50 per cent inhibition (I 50) <strong>of</strong> anenzymatic reaction. BiochemicalPharmacology. 22: 3098-3108.5. Whitebread, S., Hamon J., Bojanic, D. <strong>and</strong>Urban, L. (2005). Keynote review: In vitrosafety pharmacology pr<strong>of</strong>iling: an essentialtool for successful drug development. DrugDiscovery Today, 10: 1421-1433.6. Joshi, K.A., Patil D.D. <strong>and</strong> Gejji S.P. (2009).Molecular electrostatic potentials in aromaticsubstituted 4-hydroxyquino-2-lones: Glycine/NMDA receptor antagonists. Journal <strong>of</strong>Molecular Modeling. 15: 383–390.7. Toutain, P-L. (2002). Pharmacokinetic/pharmacodynamic integration in drugdevelopment <strong>and</strong> dosage-regimenoptimization for veterinary medicine. TheAAPS Journal. 4: 160-188.8. Stahl, M., Guba, W. <strong>and</strong> Kansy, M. (2006).Integrating molecular design resources withinTool development for Prediction <strong>of</strong> pIC 50values