full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1104-1109 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1105the chemical assay is prepared <strong>and</strong> evaluated.Both nanomolar <strong>and</strong> micromolar describes thesame value but different due to the power <strong>of</strong>conic (5). It exhibits how much <strong>of</strong> a meticuloussubstance/molecule is desirable to inhibitsome biological progression by 50% (6), <strong>and</strong>also the quantitative measure indicate howmuch, a specific drug or other core is neededto hinder a given biological process (7). It isuniversally used to calculate a antagonist drugpotency in pharmacological study (8). It is alsoconverted to the pIC 50scale (-log IC 50) (13),in which higher values indicate exponentiallygreater potency (9, 10). The IC 50values arehaving variant values, which differ from onelig<strong>and</strong> to another (11), it depends on minimumeffective concentration <strong>of</strong> the drug towards thediseased protein (12). These values are broadin range, for molecular modeling <strong>and</strong> pharmacologicalfield the IC 50values are convertedin to pIC 50values (13). For this purpose thereis formula to convert the IC 50value to pIC 50value with representing the logarithmic values.pIC 50= log1/IC 50(1)pIC 50= -log IC 50(2)The above formula can change theIC 50values into wide range to pIC 50narrowrange. But the IC 50values are given innanomolar <strong>and</strong> micromolar scale (14-15) so,again the nanomolar values also should includedin the pIC 50.So the formula againchanges according to nanomolar value, this isthe formula for nanomolar conversion <strong>of</strong> IC 50values to pIC 50values (16).pIC 50= -log IC 50*10 -9 (3)These 1, 2 <strong>and</strong> 3 are basic formula for the conversion<strong>of</strong> the IC 50into pIC 50value innanomolar. The bioassay values are interpretedas different numbers, which differ from onecompound to another compound even in sameassay. pIC 50values is changing the IC 50valuestowards interpretation for all QSAR <strong>and</strong>Pharmacophore analysis. Till date there is nos<strong>of</strong>tware or tool is available to convert IC 50topIC 50values. Here we have generated thescript <strong>and</strong> formula to create an algorithm forpIC 50as web based tool, for the molecularmodeling <strong>and</strong> pharmacological calculation. As<strong>of</strong> now, this is first report on conversion <strong>of</strong>IC 50value to pIC 50value as a web tool. Possibilities<strong>of</strong> generating the range <strong>of</strong> pIC 50valuesplaying the theoretical role, which distinguishthis from IC 50values.Materials <strong>and</strong> methodsJavaScript : JavaScript allows website designerswith moderate programming skills to add capabilitiesto their web pages, including instant userfeedback, advanced form processing, pop-up windows,advanced frame applications <strong>and</strong> muchmore. The basic elements <strong>of</strong> JavaScript language<strong>and</strong> several techniques to take web pages to thenext level, reliable coding accuracy is required inWebPages. Here we code an algorithm based onJavaScript, which are embedded with functionalvalues, <strong>and</strong> additionally this tool will work bothonline <strong>and</strong> <strong>of</strong>fline. scriptalgorithm place here (17, 18).Algorithm Coding : The algorithm has been generatedusing the JavaScript, with mathmaticalfunctions, using 3 formulas as nanomolar (nM)function as describe above. According to this algorithmdevelopment the input value will be acceptedin nanomolar <strong>and</strong> another for micromolaronly.Implementation <strong>of</strong> the Algorithm : The algorithmdeveloped as base <strong>of</strong> JavaScript, the abovementioned formula is detail source for developingthe tool for pIC 50calculator based on pro-Ch<strong>and</strong>rabose Selvaraj et al