full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and PharmacyVol. 5 (2) 1098-1103 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1102can be proved biologically. Spectrophotometricscanning of crystalline alternaric acid showed adistinct peak in diluted samples whereas; inundiluted sample many peaks were seen. Thus,the presence of alternaric acid was confirmed bythis simple analytical technique.Fig. 6. Shows in vivo bioassay Treatedis shown to be 12-(5, 6-dihydro-4-hydroxy-6-methyl-2-oxopyran-3-yl)-4, 5-dihydroxy-3-methyl-9-methylene-12-oxododec-6-ene-5-carboxylicacid. (14).Increase in alternaric acid yield wasmeasured by weighing dried crystals of alternaricacid. The yield obtained was five times higher bythis method than by the previous methodsreported. The presence of colorless alternaric acidin crystalline form could be visualizedmicroscopically under 10X and 40X magnificationsand also through naked eyes. HPTLC is a fastand reliable technique. The advantage of HPTLCis that quantities of substances too small to bedetected by chemical methods could be separatedand thereafter their biological activity can bemeasured by performing bioassay. The presenceof alternaric acid in crude FCF was confirmedby comparing both the Rf values of crude FCFand that of crystalline alternaric acid. Scanningof HPTLC foils showed a distinct peak whichproves the presence and purity of alternaric acid.The detached leaf assay is a simplemethod which allows controlled inoculation withspecific isolates, without destroying the wholeplant. The result of detached leaf bioassay showedsimilar symptoms as shown by the A. solaniinfected leaves in field. The symptoms appearedvery fast and were distinct. Further virulence ofcrystals was checked and confirmed by in vivobioassay. Thus the presence of alternaric acidAcknowledgements We are highly thankful toDr. Subhash Patel, Anand Agricultural University,India for providing the A. solani isolate.References1. FAOSTATdata,2005..http://faostat.fao.org/faostat/form?collection=Production.Crops.Primary & Domain=Production&servlet=1&hasbulk=&version = ext&language=EN. Last updated February 2005.2. Datar, V.V. and Mayee, C.D. (1972).Conidial dispersal of Alternaria solani intomato. Indian Phytopathology 35: 68-70.3. Basu, P.K. (1974). Measuring early blight,its progress and influence on fruit losses innine tomato cultivars. Can Plant Dis Surv.54: 45-51.4. Jones, J.B., Jones, J.P., Stall, R.E. and Zitter,T.A. eds. (1993).Compendium of TomatoDiseases. St Paul, Minnesota, USA:American Phytopathological Society.5. SheRf, A.F. and MacNab, A.A. (1986).Vegetable Diseases and Their Control. JohnWiley & Sons, New York.6. Montemurro, N. and Visconti, A. (1992).Alternaria metabolites – chemical andbiological data. In:Chelkowski J, Visconti A(eds) Alternaria biology, plant disease andmetabolites. Elsevier, Amsterdam, pp 449-558.7. Brian, P.W., Elson, G.W., Hemming, H.G.and Wright, J.M. (1952). The phytotoxicproperties of alternaric acid in relation toIsolation of Alternaric acid