full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and PharmacyVol. 5 (2) 1193-1205 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)locate the hidden platform kept in north quadrantfrom all the three directions suggesting theretention of earlier acquisition (Fig. 6). As in thecase of acquisition and reversal phases, Pb+Mnexposureexhibited greater impairments in workingmemory also.Acetylcholinesterase: Among the different brainregions studied, the specific activity ofsynaptosomal AChE in control rats was recordedhighest in hippocampus, followed by cerebralcortex and cerebellum. Incase of Pb+Mn treatedanimals, a decrease in the activity of AChE wasobserved in all the three brain regions i.e.,hippocampus followed by cerebral cortex andcerebellum.The animals which received calciumtogether with Pb+Mn indicated significantrecovery in AChE activity in all the three brainregions (Fig. 7).Adenosine triphosphatases: The specificactivity of Na + K + ATPase was recorded highestin cerebral cortex (0.282), followed byhippocampus (0.217) and cerebellum (0.146) inthe control rats. Incase of Pb+Mn treated rats, adecrease in the Na + K + ATPase activity wasrecorded in all the brain regions, i.e., cerebralcortex (0.211) followed by hippocampus (0.172)and cerebellum (0s.101). However, calciumsupplementation to Pb+Mn, reversed the inhibitoryeffect of Pb+Mn in all the brain regions; i.e.,cerebral cortex (0.256) followed by hippocampus(0.198) and cerebellum (0.124) (Fig.8).1199activity, i.e., hippocampus (0.768) followed bycerebral cortex (0.754) and cerebellum (0.601)(Fig.9).Fig.7. Effect of Pb+Mn exposure on AChE activity incerebral cortex, hippocampus and cerebellum. Ratswere exposed to 0.2% lead acetate through drinkingwater and Mn (2.5 mg/kg) intraperitonially daily for aperiod of 21 days. Calcium (0.02%) was added in 0.2%Pb water. Each bar represents mean ± SD (n = 6). Thechanges (values marked with asterisk *) are significantat p