full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1193-1205 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)locate the hidden platform kept in north quadrantfrom all the three directions suggesting theretention <strong>of</strong> earlier acquisition (Fig. 6). As in thecase <strong>of</strong> acquisition <strong>and</strong> reversal phases, Pb+Mnexposureexhibited greater impairments in workingmemory also.Acetylcholinesterase: Among the different brainregions studied, the specific activity <strong>of</strong>synaptosomal AChE in control rats was recordedhighest in hippocampus, followed by cerebralcortex <strong>and</strong> cerebellum. Incase <strong>of</strong> Pb+Mn treatedanimals, a decrease in the activity <strong>of</strong> AChE wasobserved in all the three brain regions i.e.,hippocampus followed by cerebral cortex <strong>and</strong>cerebellum.The animals which received calciumtogether with Pb+Mn indicated significantrecovery in AChE activity in all the three brainregions (Fig. 7).Adenosine triphosphatases: The specificactivity <strong>of</strong> Na + K + ATPase was recorded highestin cerebral cortex (0.282), followed byhippocampus (0.217) <strong>and</strong> cerebellum (0.146) inthe control rats. Incase <strong>of</strong> Pb+Mn treated rats, adecrease in the Na + K + ATPase activity wasrecorded in all the brain regions, i.e., cerebralcortex (0.211) followed by hippocampus (0.172)<strong>and</strong> cerebellum (0s.101). However, calciumsupplementation to Pb+Mn, reversed the inhibitoryeffect <strong>of</strong> Pb+Mn in all the brain regions; i.e.,cerebral cortex (0.256) followed by hippocampus(0.198) <strong>and</strong> cerebellum (0.124) (Fig.8).1199activity, i.e., hippocampus (0.768) followed bycerebral cortex (0.754) <strong>and</strong> cerebellum (0.601)(Fig.9).Fig.7. Effect <strong>of</strong> Pb+Mn exposure on AChE activity incerebral cortex, hippocampus <strong>and</strong> cerebellum. Ratswere exposed to 0.2% lead acetate through drinkingwater <strong>and</strong> Mn (2.5 mg/kg) intraperitonially daily for aperiod <strong>of</strong> 21 days. Calcium (0.02%) was added in 0.2%Pb water. Each bar represents mean ± SD (n = 6). Thechanges (values marked with asterisk *) are significantat p