full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (2) 1084-1097 April 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1088Blood glucose levels after subcutaneousadministration <strong>of</strong> insulin nanoparticles: InsulinLoaded nanoparticles (10 IU/kg) prepared by theabove different methods were injected to feddiabetic rats. Free insulin (10 IU/kg), unloadednanoparticles <strong>and</strong> PBS (pH 7.4) were alsoadministered in control animals. Samples <strong>of</strong> 1drop <strong>of</strong> blood were withdrawn from the tail veinat different time intervals <strong>and</strong> evaluated for theglucose levels using OneTouch Ultra 2 glucometerbefore injection <strong>and</strong> 0.5, 1, 1.5, 2, 3, 6, 8, 10, 12,24, 48, <strong>and</strong> 72 hour post injection. Rats weremaintained fasted during the experiment up to 12h <strong>and</strong> fed thereafter.Statistical analysis: The mean <strong>and</strong> the st<strong>and</strong>arderrors were calculated for each group. For groupcomparisons a one-way analysis <strong>of</strong> variancefollowed by the least significant difference (LSD)as a post-hoc test was applied, using SPSSprogram version 9 s<strong>of</strong>tware. When P