Revision ISO 10272 Detection and enumeration of ... - SVA

Direct plating on selective agarISO 10272-1C: detectionproposal-For caecal of faecal samples use a loop or a sterile swab tobring some of the well-mixed sample material onto the first halfof a mCCDA plate. Use another loop to streak out on the secondhalf of the plate.-For all other samples, add an appropriate amount of liquid (e.g.MRD or Preston broth), for example 1 to 1 w/w, mix well, andeither streak the plate using a loop, or dispense a suitablevolume and spread it over the mCCDA plate.23