Revision ISO 10272 Detection and enumeration of ... - SVA
Revision ISO 10272 Detection and enumeration of ... - SVA
Revision ISO 10272 Detection and enumeration of ... - SVA
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Direct plating on selective agar<strong>ISO</strong> <strong>10272</strong>-1C: detectionproposal-For caecal <strong>of</strong> faecal samples use a loop or a sterile swab tobring some <strong>of</strong> the well-mixed sample material onto the first half<strong>of</strong> a mCCDA plate. Use another loop to streak out on the secondhalf <strong>of</strong> the plate.-For all other samples, add an appropriate amount <strong>of</strong> liquid (e.g.MRD or Preston broth), for example 1 to 1 w/w, mix well, <strong>and</strong>either streak the plate using a loop, or dispense a suitablevolume <strong>and</strong> spread it over the mCCDA plate.23