Revision ISO 10272 Detection and enumeration of ... - SVA

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Questions from CEN/TC 275/WG 6- Optimum incubation time for Preston broth – 24 h- Ratio of headspace to enrichment volume – no mention of theuse of reduced headspace- Addition of blood (0, 1 or 5%) to enrichment media – Bolton +5% blood + FBP; Preston broth + 5% blood- Do Preston or Bolton broth select for different Campylobacterspecies – not clear at the moment- Should skin or pieces of meat be separated from enrichmentbroth by the use of filter bags – better in e.g. ISO 688716
ISO 10272-4: Samples from primary production(from animals and their environment)For the development of part 4 of EN ISO 10272, CEN/TC275/WG6asked the Project Leader to liaise with TAG5 (meeting AHVLA,20/21 October 2011)Samples from primary production:- Detection bydirect plating (e.g. caecal samples) acc. ISO 10272-1BISO 10272-1A or ISO 10272-1B (depending oncontaminating microflora)- Enumeration using ISO 10272-2
Page 1 and 2: ISO 10272Detection and enumeration
Page 3 and 4: Ad Hoc Group CampylobacterBelgium:
Page 6 and 7: EnrichmentAd Hoc Group Campylobacte
Page 8 and 9: EnrichmentAd Hoc Group Campylobacte
Page 10 and 11: Ad Hoc Group Campylobacter - propos
Page 12 and 13: Ad Hoc Group Campylobacter - propos
Page 14 and 15: Microaerobic incubationmicroaerobic
Page 18 and 19: ISO 10272-1: detectionproposal- Is
Page 20 and 21: 20ISO 10272-1A: detectionproposal
Page 22 and 23: 22ISO 10272-1C: detectionproposal
Page 24 and 25: Further development of revised ISO
Page 26: Thank you for your attentionenne.de

Revision ISO 10272 Detection and enumeration of ... - SVA

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