Revision ISO 10272 Detection and enumeration of ... - SVA
Revision ISO 10272 Detection and enumeration of ... - SVA
Revision ISO 10272 Detection and enumeration of ... - SVA
- No tags were found...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Earlier proposal:Microaerobic incubationNOTE 2 As an alternative to incubation in a microaerobicatmosphere, the enrichment can be done in tightly closed containersfilled with enrichment broth, leaving a headspace <strong>of</strong> about 20% <strong>of</strong> thetotal volume <strong>of</strong> the container.Proposed text:NOTE 2As an alternative to incubation in a microaerobic atmosphere, theenrichment can be done in tightly closed containers filled withenrichment broth <strong>and</strong> having a reduced headspace.This alternative should only be applied, when there is sufficientevidence <strong>of</strong> the creation <strong>of</strong> correct microaerobic conditions.Final proposal:Delete the note, as it is impossible to st<strong>and</strong>ardize microaerobic incubationby using bottles with reduced headspace.