Revision ISO 10272 Detection and enumeration of ... - SVA

Earlier proposal:Microaerobic incubationNOTE 2 As an alternative to incubation in a microaerobicatmosphere, the enrichment can be done in tightly closed containersfilled with enrichment broth, leaving a headspace of about 20% of thetotal volume of the container.Proposed text:NOTE 2As an alternative to incubation in a microaerobic atmosphere, theenrichment can be done in tightly closed containers filled withenrichment broth and having a reduced headspace.This alternative should only be applied, when there is sufficientevidence of the creation of correct microaerobic conditions.Final proposal:Delete the note, as it is impossible to standardize microaerobic incubationby using bottles with reduced headspace.