Revision ISO 10272 Detection and enumeration of ... - SVA

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Microaerobic incubationmicroaerobic atmosphere withoxygen content 5 % 2 %carbon dioxide 10 % 3 %optional hydrogen ≤10 %with the balance nitrogenISO 10272-1:20066.14NOTE 2As an alternative to incubation in a microaerobic atmosphere, theenrichment can be incubated in screw-capped bottles or flasks filledwith enrichment broth, leaving a headspace of less than 2 cm, andtightly closing the caps.
Earlier proposal:Microaerobic incubationNOTE 2 As an alternative to incubation in a microaerobicatmosphere, the enrichment can be done in tightly closed containersfilled with enrichment broth, leaving a headspace of about 20% of thetotal volume of the container.Proposed text:NOTE 2As an alternative to incubation in a microaerobic atmosphere, theenrichment can be done in tightly closed containers filled withenrichment broth and having a reduced headspace.This alternative should only be applied, when there is sufficientevidence of the creation of correct microaerobic conditions.Final proposal:Delete the note, as it is impossible to standardize microaerobic incubationby using bottles with reduced headspace.
Page 1 and 2: ISO 10272Detection and enumeration
Page 3 and 4: Ad Hoc Group CampylobacterBelgium:
Page 6 and 7: EnrichmentAd Hoc Group Campylobacte
Page 8 and 9: EnrichmentAd Hoc Group Campylobacte
Page 10 and 11: Ad Hoc Group Campylobacter - propos
Page 12 and 13: Ad Hoc Group Campylobacter - propos
Page 16 and 17: Questions from CEN/TC 275/WG 6- Opt
Page 18 and 19: ISO 10272-1: detectionproposal- Is
Page 20 and 21: 20ISO 10272-1A: detectionproposal
Page 22 and 23: 22ISO 10272-1C: detectionproposal
Page 24 and 25: Further development of revised ISO
Page 26: Thank you for your attentionenne.de

Revision ISO 10272 Detection and enumeration of ... - SVA

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