Production of Haploid & Use of Doubled Haploids in Wheat Breeding

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Efficiency of Haploid Production• Fertilization: 80 - 85%• Embryo formation: 20 - 30%• Embryo germination: 45 - 60%• Haploid green plants: 8 - 15%• Doubling efficiency: 80 - 85%• Doubled haploid plants: 6 - 10%
Technical Difficulties• Unusual regeneration (13%)– Regeneration without shoot– Regeneration with glass or white shoot– Regeneration without roots• Various Loss during colchicine treatment (30%)• Five fertile plants were obtained per 100 floretspollinated (average 2 doubled haploid plants perpollinated head)
Page 4 and 5: Breeding Using Doubled Haploid Syst
Page 6: Backcrossing or Pyramiding• Paren
Page 10 and 11: Application of Doubled HaploidGenet
Page 12 and 13: Methodologies for inducing haploid
Page 14 and 15: Barley x H. Bulbosum• Conventiona
Page 16 and 17: Proceduresof Wheat x Maize Hybridiz
Page 18 and 19: Pollination• Fresh pollen• Poll
Page 20 and 21: Embryo Culture• Excise embryos fr
Page 22 and 23: Doubled Haploid Production• Colch
Page 24 and 25: Colchicine Treatment• Colchicine
Page 26 and 27: Colchicine Treatment• Colchicine
Page 28 and 29: After Colchicine Treatment
Page 30 and 31: Factors?• Pre-hybridization• Ge
Page 32 and 33: Table1. Haploid production by wheat
Page 34 and 35: Haploid Productionin Twelve Wheat F
Page 38 and 39: Increase the Efficacyof Wheat x Mai
Page 40 and 41: Summaryof Wheat x Maize System• S

Production of Haploid & Use of Doubled Haploids in Wheat Breeding

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