Production of Haploid & Use of Doubled Haploids in Wheat Breeding

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Haploid Productionin Twelve Wheat F 1 Populations• Significant genotype differences were found in F 1 crossesfor the efficiency of haploid production, based on thepercentage of embryo germination and the percentage ofhaploid green plants regenerated• Roane, a scab-tolerant variety, and Pioneer 2684 were thebest parents for doubled haploid production
Table 3. Effect of pre-cold treatment for haploid regenerationwith wheat x maize hybridization in two wheat F 1 crossesconducted in 1999 and 2000*, Blacksburg, Virginia.PedigreeSeedsExcised(No.)EmbryosRescued(No.)HaploidObtained(No.)EmbryoFormation(%)HaploidRegeneration(%)1999 2000 1999 2000 1999 2000 1999 2000 1999 2000Madison x W14 452 2202 48 320 12 144 11 15 25 45Pioneer 2684 x W14 587 1473 140 258 61 144 24 18 44 56Total 1049 3675 188 578 73 288Average 17.5 16.5 34.5 50.5* All embryos were given pre-cold treatment in 2000 greenhouse test.
Page 4 and 5: Breeding Using Doubled Haploid Syst
Page 6: Backcrossing or Pyramiding• Paren
Page 10 and 11: Application of Doubled HaploidGenet
Page 12 and 13: Methodologies for inducing haploid
Page 14 and 15: Barley x H. Bulbosum• Conventiona
Page 16 and 17: Proceduresof Wheat x Maize Hybridiz
Page 18 and 19: Pollination• Fresh pollen• Poll
Page 20 and 21: Embryo Culture• Excise embryos fr
Page 22 and 23: Doubled Haploid Production• Colch
Page 24 and 25: Colchicine Treatment• Colchicine
Page 26 and 27: Colchicine Treatment• Colchicine
Page 28 and 29: After Colchicine Treatment
Page 30 and 31: Factors?• Pre-hybridization• Ge
Page 32 and 33: Table1. Haploid production by wheat
Page 36 and 37: Efficiency of Haploid Production•
Page 38 and 39: Increase the Efficacyof Wheat x Mai
Page 40 and 41: Summaryof Wheat x Maize System• S

Production of Haploid & Use of Doubled Haploids in Wheat Breeding

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