Production of Haploid & Use of Doubled Haploids in Wheat Breeding

Colchicine Treatment• Colchicine solution: 1g colchicine powder (1%), 20 ml dimethylsulphoxide(DMSO), and 10 drop of Tween 20 per liter• Direct – treatment– save time but may lose some haploid plants• Post - treatment after vernalization– take more time but may save more haploid plants– vernalize green plants right after regeneration– transfer haploid plants to vermiculite after 6-8 weeks– treat them with colchicine solution when plants become healthy