Production of Haploid & Use of Doubled Haploids in Wheat Breeding
Production of Haploid & Use of Doubled Haploids in Wheat Breeding
Production of Haploid & Use of Doubled Haploids in Wheat Breeding
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<strong>Doubled</strong> <strong>Haploid</strong> <strong>Production</strong>• Colchic<strong>in</strong>e treatment: Colchic<strong>in</strong>eprevents cell division by <strong>in</strong>activat<strong>in</strong>g thesp<strong>in</strong>dle mechanism. It doesn’t affectchromosome replication, but doesprolong the time for mitosis.• Cells at meristematic stage are sensitiveto the treatment; therefore, a mixture <strong>of</strong>haploid and doubled haploid will beobta<strong>in</strong>ed.