full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (1) 1043-1053 January 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1047vancomycin (VA 5 ), kanamycin (K 30 ), novobiocin(Nv 5 ),tetracycline (T 10 ), neomycin (N 30 ),erythromycin (E 15 ). Only one isolate Y8 showedthe resistance against the tetracycline (T 10 ) <strong>and</strong>kanamycin (K 30 ).The identification <strong>of</strong> Streptomyces isolateswas carried out by using morphological,physiological <strong>and</strong> biochemical characteristics(Table-3). All the Streptomyces were found togram positive when subjected to Gram staining.Fig. 2 showed the microscopic observation(1000X) <strong>of</strong> Streptomyces isolates. Probabilisticidentification matrix for Streptomyces that basedon various morphological, physiological <strong>and</strong>biochemical characteristic, antibiosis <strong>and</strong>resistance to antibiotics, was calculated by usingPIBWin s<strong>of</strong>tware, Bacterial IdentificationProgram Version: 1.9.2 (18). Most active isolateY8 was identified as Streptomyces rimosus withthe ID score 0.99, three isolates No A8, MS02<strong>and</strong> D13 were identified as Streptomycesantibioticus with ID score 0.99, 1.0 <strong>and</strong> 1.0respectively. Isolate MS38 was identified asStreptomyces chromogenus with ID score 1.0,isolate No D15, MS09 <strong>and</strong> P2N were identifiedFig. 2. The microscopic observation <strong>of</strong> Streptomycesisolates under the light microscope (1000X) (A) Y8(Streptomyces rimosus) (B) A8 (Streptomycesantibioticus) (C) D13 (Streptomyces antibioticus)(D) M8 (Streptomyces lydicus) (E) MS02 (Streptomycesantibioticus) (F) M1 (Streptomyces cyaneus) (G)M9 (Streptomyces phaeochromogenes) (F) MS41(Streptomyces filipinensis) (H) MS03 (Streptomycesexfloliatus) (I) D15 (Streptomyces rouchi) (J) M7(Streptomyces chrom<strong>of</strong>uscus) (K) P2N (Streptomycesrouchi) (L) MS14 (Streptomyces atroolivaceus)Fig. 3. Antimicrobial activity <strong>of</strong> Streptomyces isolates(A) A8, MS02, MS41, Y8, D13, MS38 showingactivity against B.subtilis (MTCC 1789) (B) A8, MS02,MS41, Y8, D15, MS38 showing activity againstB.cereus (MTCC 1305) (C) Y8, MS38, A8, MS02 showingactivity against A. niger (MTCC 872) (D) A8, Y8,MS38 showing activity against C.albicans (MTCC1637) by agar well diffusion method.Isolation <strong>and</strong> Characterization <strong>of</strong> Streptomyces sp.
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