full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (1) 998-1003 January 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1000Cultural <strong>and</strong> physiological characterizations<strong>of</strong> Bb 36Streptomyces isolate : Streptomycesisolate (Bb 36strain) was characterizedmorphologically <strong>and</strong> physiologically according tothe International Streptomyces project (ISP) (11)<strong>and</strong> as described by Saadoun et al. (9).ResultsDetermination <strong>of</strong> optimum culture media forinhibitory compound(s) production : When theactivity <strong>of</strong> Bb 36Streptomyces isolate wascompared to 23 st<strong>and</strong>ard antibiotics, data revealedthat this isolate may not produce any <strong>of</strong> the testedantibiotics except cefotaxime <strong>and</strong> cefuroximesodium. Table 1 shows several broth media thathave been examined to test the ability <strong>of</strong> the activeStreptomyces strain Bb 36to produce inhibitorybioactive compounds against the tested pathogens.Data showed that strain Bb 36optimally produceinhibitory bioactive compound(s) against E. coli<strong>and</strong> S. aureus after 5 days when cultured inYEME broth with an inhibition zone diameter <strong>of</strong>8 <strong>and</strong> 13 mm, respectively. Also, growth asmeasured by dry weight biomass production inthe YEME broth medium was followed for 14days (Table 1). Results from Table 1 demonstratedthat Bb 36Streptomyces isolate prefer to producethese inhibitory substance(s) in YEME brothmedium at the expense <strong>of</strong> growth.Partial isolation <strong>and</strong> purification <strong>of</strong> the activecompound(s) : It is necessary to extract <strong>and</strong>purify the substance responsible for the antibioticactivity, so further tests for evaluation werecarried out with a simple ethanol extraction <strong>of</strong>the antibiotic from cells grown in liquid cultures.Table 2 shows the MIC <strong>of</strong> the evaporated watersolubleethanol extract against S. aureus <strong>and</strong> E.coli. Ethanol extraction <strong>and</strong> evaporation <strong>of</strong> theaTable 1. Activity <strong>of</strong> Bb 36isolate at different incubation intervals <strong>and</strong> culture media.Isolate Broth Final pH Dry Weight (mg/ml) Activity (mm) bMediumDays E. coli S. aureus5 7 14 5 7 14 5 7 14 5 7 14Bb36 TYE 8.53 8.75 8.65 1.2 0.0 0.0 13 - - 10 - -NB 8.36 8.66 8.76 0.2 0.6 1.0 - - - - - -YEME 6.36 6.72 8.15 2.4 2.6 3.5 8 8 8 13 11 11ISS 6.42 6.70 8.08 2.4 2.9 3.3 - - - - - -aTYE: Tryptone Yeast Extract; NB: Nutrient Broth; YEME: Yeast Extract Malt Extract;ISS: Inorganic Salt StarchbNo activity was recorded at 3 <strong>and</strong> 21 daysTable 2. Activity <strong>of</strong> ethanol evaporate extract <strong>of</strong> Streptomyces B36 isolate.Extract Concentration (mg/ml)Bacteria 10 9 8 7 6 5 4 3 2 1S. aureus 13 a 13 13 13 13 11 - - - -E. coli 8 - - - - - - - - -aDiameter <strong>of</strong> inhibition zone (mm, mean value, N= 3).Inhibitory compounds producing against multiresistant bacterial pathogens