full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong>Vol. 5 (1) 982-992 January 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)987Fig. 4. Effect <strong>of</strong> nitrogen on exoglucanase productionby Aspergillus sp. RCAL-5 under following conditions(pH 4.5, temp. 28 o C, agitation150 rpm , wheatbran 10 gl -1 , incubation time (4 days)).Fig. 6. Effect <strong>of</strong> temperature on exoglucanase productionby Aspergillus sp. RCAL-5 under followingconditions. (agitation 150 rpm, wheat bran 10 g l -1 ,yeast extract 2.5 g l -1 , pH 4.5, incubation time (4 days)).Fig. 5. Effect <strong>of</strong> pH on exoglucanase production byAspergillus sp. RCAL-5 under following conditions.(temp. 28 °C, agitation 150 rpm, wheat bran 10 g l -1 ,yeast extract 2.5 g l -1 , incubation time (4 days)).Fig. 7. Effect <strong>of</strong> agitation on exoglucanase productionby Aspergillus sp. RCAL-5 under following conditions.(temp. 28 o C, wheat bran 10 g l -1 , yeast extract2.5 g l -1 , pH 4.5, incubation time (4 days)).maximum cellulase production (0.775 IU ml -1 )using saw dust as carbon source from A.nigergrown at 28°C.Recently, Deshp<strong>and</strong>e et al. (17) observedmaximum cellulase activity (0.22±0.04 IU ml -1 )at the incubation temperature <strong>of</strong> 30 o C.Effect <strong>of</strong> AgitationEffect <strong>of</strong> different agitation rates rangingfrom 50-250 rpm were studied for maximumFPase (0.81 FPU ml -1 ) production under theconditions (temp. 28 o C, wheat bran 10 g l -1 , yeastextract 2.5 g l -1 , pH 4.5). From the Figure 7, it isCh<strong>and</strong>ra Sekhar et al