Boston - American Association for Thoracic Surgery

AMERICAN ASSOCIATION FOR THORACIC SURGERYF17. Comparative Glycomic Profiling in Esophageal AdenocarcinomaZane Hammoud, 1 Yehia Mechref, 2 Ahmed Hussein, 2 Slavka Bekesova, 2Min Zhang, 2 Kenneth Kesler, 3* Robert Hickey, 3 Milos Novotny 21. Cardiothoracic Surgery, Henry Ford Health System, Detroit, MI, USA;2. Indiana University, Bloomington, IN, USA; 3. Indiana University Schoolof Medicine, Indianapolis, IN, USAInvited Discussant: Arjun PennathurOBJECTIVE: Aberrant glycosylation has been implicated in various types of cancersand changes in glycosylation may be associated with signaling pathways duringmalignant transformation. Cancerous cells with altered glycosylation of their surfaceproteins shed such proteins into the circulating fluids. Glycomic profiling ofsuch fluids would reveal the altered glycosylation. We performed glycomic profilingof serum from patients with no known disease, patients with high grade dysplasia,and patients with esophageal adenocarcinoma in an attempt to delineate distinctdifferences in glycosylation between these groups.METHODS: Serum samples from patients with Barrett’s metaplasia (N = 5), highgradedysplasia (HGD, N = 11) and esophageal adenocarcinoma (EAC, N = 50)were collected; samples from 18 healthy volunteers were used as control. SerumN-glycans were enzymatically released using PNGase F. Samples were then appliedto both C18 Sep-Pak ® cartridges and activated charcoal cartridges. N-glycans werepermethylated and then spotted directly on the MALDI plate and mixed withequal volume of DHB-matrix. Mass spectra were acquired using the AppliedBiosystems 4800 MALDI TOF/TOF Analyzer. The obtained MALDI-MS data wereprocessed using DataExplorer files listing m/z values and intensities.TUESDAYMorning* AATS Member147