Boston - American Association for Thoracic Surgery

More documents

Recommendations

Info

89 TH ANNUAL MEETING MAY 9–MAY 13, 2009BOSTON, MASSACHUSETTSF13. Replacement of the Trachea with Fully Bioengineered Graft in PigsTetsuhiko Go, 1 Philipp Jungebluth, 1 Adelaide Asnaghi, 2 Sara Mantero, 2 Maria-Teresa Conconi, 3 Antony Hollander, 4 Martin Birchall, 4 Paolo Macchiarini 1*1. General Thoracic Surgical Experimental Laboratory, Universitat de Barcelona,Barcelona, Spain; 2. Department of Bioengineering, Politecnico di Milano, Milano,Italy; 3. Pharmaceutical Science, University of Padua, Padua, Italy; 4. Departmentof Cellular and Molecular Medicine, School of Medical Sciences, Bristol, UnitedKingdomInvited Discussant: Yolonda L. ColsonOBJECTIVE: Evaluate the outcome of a fully bioengineered tracheal graft in pigs.METHODS: Non-immunogenic tracheal matrices were obtained via detergentenzymaticmethod (DEM) from pig donors. MHC-unmatched animals (weighing65 ± 4 Kg) were divided into four groups (each, n = 5) and 6 cm of their tracheasreplaced with a DEM matrix alone (group I) or seeded with recipients autologouschondrocytes (group II) or epithelial cells (group III), or both (groupIV). Epithelialcells (via bronchial-epithelial biopsies) and stem cells (bone marrow aspiration)were harvested from recipients and in-vitro cultured. Stem cells were differentiatedinto chondrocytes using specific growth factors. Both cell types were seeded simultaneouslyusing a novel bioreactor allowing dynamic and physiological cell culture.Pigs were observed during study period of 60 days via bronchoscopy, blood samplesand biopsies. Grafts were evaluated mechanically and immunohistologicallypre-implantation and post-mortem.RESULTS: Matrices were completely covered with both chondrocytes and epithelialcells within 72 hours using the new device. Extent of seeding affected animalslife time and outcome significantly (p < 0.05) (group I: 11 ± 2days; II: 29 ± 4 days;III: 34 ± 4 days; IV: 60 ± 1 days). Animals died due to severe respiratory disorders(group I), grafts bacteria contamination (group II) or stenosis and anastomoticfailure (group III). Group IV animals showed bronchoscopically healthy and blandcovered graft surface without any collapse of the graft. No rejection signs occurredin this immunosuppression-free model. Grafts strain abilities were equal to nativetracheas (tissue deformation: 211 ± 13 vs 206 ± 12%).CONCLUSION: The obtained bioengineered tracheal graft demonstrated itshigh potential as airway replacement.* AATS Member142
AMERICAN ASSOCIATION FOR THORACIC SURGERYF14. DYRK2, a Dual-Specificity Tyrosine-(Y)-Phosphorylation-Regulated Kinase Gene, Expression can be a Predictive Markerfor Chemotherapy in Non-small Cell Lung CancerShin-ichi Yamashita, Katsunobu KawaharaSurgery II, Oita University Faculty of Medicine, Yufu, JapanInvited Discussant: David JablonsOBJECTIVE: Several predictive markers of treatment and survival benefit werereported such as ERCC1 in NSCLC (non-small cell lung cancer). We report herethe correlation between clinicopathological factors in non-small cell lung cancer(NSCLC) and expression of DYRK2, a dual-specificity tyrosine-(Y)-phosphorylationregulated kinase gene, furthermore, the possibility to predict benefit from chemotherapyfor patients in recurrent NSCLC.METHODS: DYRK2 expression in 157 patients with NSCLC were evaluated byimmunohistochemistry (IHC) and quantitative RT-PCR. The correlation betweenthe expression levels of this gene and clinicopathological factors were investigated.In the other series, forty patients with recurrent disease after surgery received severalcombinations of platinum-based chemotherapy. Chemotherapy effectiveness wasevaluated according to RECIST criterion and the relationship between clinicaleffectiveness and the expression levels of this gene by IHC were evaluated.TUESDAYMorningRESULTS: We could not find any correlation between age, sex, pathological stage,tumor size, histological type and DYRK2 expression. However, this gene expressionwas significantly related to nodal metastases (P < 0.05). Overall response rate is22.2% (4 out of 18) in DYRK2 positive group compared with 4.5% (1 out of 22) innegative group. On the other hand, 17 PD (progressive disease) is consisted of 3DYRK2 positive patients and 14 DYRK2 negative patients.(p = 0.0086) The mediantime to the progression of disease was 120 days in the DYRK2 negative group, ascompared with 310 days in the DYRK2 positive group(HR = 1.984, 95% CI =[1.039–3.788], p = 0.034).CONCLUSION: Our study showed that DYRK2 has the critical role of nodalmetastases in NSCLC. Furher, patients with recurrent NSCLC and DYRK2-positive tumors derived a substantial benefit from chemotherapy, as comparedwith patients with DYRK2-negative tumors.143
Page 1 and 2:
American Associationfor Thoracic Su
Page 3 and 4:
AMERICAN ASSOCIATION FOR THORACIC S
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94: AMERICAN ASSOCIATION FOR THORACIC S
Page 143: AMERICAN ASSOCIATION FOR THORACIC S
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 227 and 228:
AATS FUTURE MEETINGSMay 1-5, 2010Me
show all

Boston - American Association for Thoracic Surgery

Create successful ePaper yourself

Delete template?

Save as template?