search for pseudomonas syringae on stone fruits in ... - Cost 873
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SEARCH FOR PSEUDOMONAS SYRINGAEON STONE FRUITS IN LITHUANIAMilda Vas<strong>in</strong>auskienė 1 , Laima Baranauskaitė 2 ,Daiva Burokienė 11Institute of Botany, Žaliųjų Ežerų 49, LT-08406, Vilnius, Lithuania2State Plant Protecti<strong>on</strong> Service, Sukilėlių 9a, LT-11351, Vilnius, Lithuania
Crops of <strong>in</strong>terest• The area under the st<strong>on</strong>e <strong>fruits</strong> is 5515 ha <strong>in</strong>Lithuania• Plums, sweet and sour cherries are grown <strong>in</strong>commercial orchards, small farms and homegardens• Home gardens (3370 ha) cover the largestpart of st<strong>on</strong>e fruit area
Comm<strong>on</strong> st<strong>on</strong>e fruit diseasesbrown rot (M<strong>on</strong>il<strong>in</strong>ia fructigena, M. laxa)silverleaf (Ch<strong>on</strong>drostereum purpureum)plum scab (Venturia carpophila)cherry leaf spot (Blumeriella jaapii)shothole (Stigm<strong>in</strong>a carpophila)sharka (Plum Pox Potyvirus)
The ma<strong>in</strong> objectives:• Evaluate the occurrence of bacterial canker<strong>on</strong> st<strong>on</strong>e <strong>fruits</strong> <strong>in</strong> orchards of the country• Collect samples of <strong>in</strong>jured plants• Isolate and characterize disease agents
Methods• Sampl<strong>in</strong>g of <strong>in</strong>jured material <strong>in</strong> st<strong>on</strong>e fruitorchards• Detecti<strong>on</strong> and isolati<strong>on</strong> of Pseudom<strong>on</strong>as<str<strong>on</strong>g>syr<strong>in</strong>gae</str<strong>on</strong>g> bacteria <strong>on</strong> nutrient dextrose andsucrose nutrient agar media• Identificati<strong>on</strong> of isolated stra<strong>in</strong>s accord<strong>in</strong>gto LOPAT and IF
Injuries <strong>on</strong> sweet cherrytwigstwigs
Injuries <strong>on</strong> plum treesBacterial canker <strong>on</strong> shootsSpots <strong>on</strong> leavesInfected sprouts
Bacterial canker<strong>on</strong> plum branches
Infected trees
Characteristics of isolated stra<strong>in</strong>sCharacteristicsPseudom<strong>on</strong>as <str<strong>on</strong>g>syr<strong>in</strong>gae</str<strong>on</strong>g> pv.12 stra<strong>in</strong>s (plums) 5 stra<strong>in</strong>s ( cherries)Col<strong>on</strong>ies <strong>on</strong> NDA whitish whitishCatalase activity + +Gram sta<strong>in</strong><strong>in</strong>g - -Fluorescence <strong>on</strong> KB + +Utilizati<strong>on</strong> of glucose O/F O OLevan producti<strong>on</strong> + +Oxidase - -Potato rot - -Arg<strong>in</strong><strong>in</strong>e-dihydralase - -Tobacco HP + +
Results• 66 stra<strong>in</strong>s were isolated from <strong>in</strong>jured st<strong>on</strong>efruit trees• 17 stra<strong>in</strong>s were positive <strong>in</strong> LOPAT tests• IF was positive with 2 stra<strong>in</strong>s from sour cherryand 8 stra<strong>in</strong>s from plum trees
Future work• Identify Pseudom<strong>on</strong>as <str<strong>on</strong>g>syr<strong>in</strong>gae</str<strong>on</strong>g> pathovars• Isolate pathogens from different parts of<strong>in</strong>jured plant• Accumulate collecti<strong>on</strong> of bacterial stra<strong>in</strong>s fromst<strong>on</strong>e fruit orchards located <strong>in</strong> the country
• Thank you <str<strong>on</strong>g>for</str<strong>on</strong>g> attenti<strong>on</strong>Thank you <str<strong>on</strong>g>for</str<strong>on</strong>g> attenti<strong>on</strong>
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