M.Balac - Pipette Clinic

Calibration Certificate.ABN 70 124 336 241Level 1, Suite 44, 48-50 George Street, Parramatta, NSW, 2150Tel:02 9635 3992 Fax:02 9639 0376 E-mail: info@pipetteclinic.com.au# PC24562.262 3.25Calibration Data - 1 µL#1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #120.99 1.03 1.03 0.99 1.00 0.99 1.00 1.00 0.99 1.00 1.01 1.001.01 1.01 1.00 1.03 1.03 1.04 1.01 1.03 1.04 1.00 1.04 1.000.98 1.03 1.01 1.04 1.03 1.04 1.01 0.99 1.03 1.00 1.02 1.031.02 1.01 1.04 1.01 1.04 1.02 1.03 1.03 1.03 0.99 1.01 1.03Mean Volume ( µL ) 1.02Mean Volume ( µL )Inaccuracy ( % ) 1.89 1.74 Imprecision CV ( % )Specification ( ± % ) 12.00 8.00 Specification ( ≤ % )Status PASS PASS StatusLinearity ( µL ) y = 1.0018x + 0.0170.Linearity ( µL )1.0018 0.01701.121.061 µL1.000.940.881 2 3 4 5 6 7 8 9 10 11 12Confidence Interval 95% 1.01 µL - 1.02 µL 99% 1.01 µL - 1.03 µLPipette DataLaboratory ConditionsPipette Manufacturer : Thermo Electron Water Temperature (ºC) 22.5Pipette Type : Finnpipette Novus 4630 Air Pressure (hPa) 1012Volume Range : 1-10 µL Relative Humidity (%) 45Serial Number : NA06546 Density Correction (µL/mg) 1.0034Customer ID: DEMO #6 Calibrated with : Sartorius ME235P auto doorCalibrated on : 26-Aug-2009 Calibrated by : Mike Balac BSc (Hons) ChemPage 1 of 3M.Balac

Calibration Certificate.ABN 70 124 336 241Level 1, Suite 44, 48-50 George Street, Parramatta, NSW, 2150Tel:02 9635 3992 Fax:02 9639 0376 E-mail: info@pipetteclinic.com.au# PC24562.262 3.25Calibration Data - 10 µL#1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #129.99 10.03 10.06 10.00 10.08 10.04 10.01 10.01 10.04 10.02 10.07 10.0010.04 10.02 10.05 10.08 10.03 10.02 10.03 10.02 10.04 10.03 10.04 10.0510.02 10.02 10.06 10.00 10.01 10.03 10.03 10.04 10.02 10.05 10.03 10.0510.03 10.01 10.04 10.02 10.03 10.06 10.00 10.03 10.04 10.03 10.07 9.99Mean Volume ( µL ) 10.04Mean Volume ( µL )Inaccuracy ( % ) 0.35 0.22 Imprecision CV ( % )Specification ( ± % ) 2.40 1.60 Specification ( ≤ % )Status PASS PASS StatusLinearity ( µL ) y = 1.0018x + 0.0170.Linearity ( µL )1.0018 0.017010.210.110 µL10.09.99.81 2 3 4 5 6 7 8 9 10 11 12Confidence Interval 95% 10.03 µL - 10.04 µL 99% 10.03 µL - 10.05µLPipette DataLaboratory ConditionsPipette Manufacturer : Thermo Electron Water Temperature (ºC) 22.5Pipette Type : Finnpipette Novus 4630 Air Pressure (hPa) 1012Volume Range : 1-10 µL Relative Humidity (%) 45Serial Number : NA06546 Density Correction (µL/mg) 1.0034Customer ID: DEMO #6 Calibrated with : Sartorius ME235P auto doorCalibrated on : 26-Aug-2009 Calibrated by : Mike Balac BSc (Hons) ChemPage 2 of 3M.Balac

Calibration Certificate.ABN 70 124 336 241Level 1, Suite 44, 48-50 George Street, Parramatta, NSW, 2150Tel:02 9635 3992 Fax:02 9639 0376 E-mail: info@pipetteclinic.com.au# PC2456Manufacturer Specification - Inaccuracy0.039.64 9.76 9.88 10.00 10.121.10 10.1910.24 10.36100 0.94 0.05 1.02 10.04 9.95 0.04 0.69 0.01 1.10 10.19 9.40 0 0.010.20 0.95 0.30 -97.96 -96.65 9.96 0.26 0.73 0.07 1.10 10.19 9.48 0.031.00 0.96 1.50 1.20 0.50 9.97 1.34 0.77 0.34 1.10 10.19 9.56 10 µL 0.183.95 0.97805.95 FAIL FAIL 9.98 5.31 0.81 1.34 1.10 10.19 9.64200.7012.18 0.98 18.32 1.82 0.21 9.99 16.35 0.85 4.12 0.94 9.89 9.72 2.1429.22 0.99 43.95 0.80 0.30 10.00 39.22 0.90 9.90 0.94 9.89 9.80 5.1554.59 1.00 82.11 FAIL PASS 10.01 73.28 0.94 18.49 0.94 9.89 9.88 9.6179.42601.01 119.47 0.02 0.02 10.03 106.62 0.98 26.90 0.94 9.89 9.964013.9990.00 1.02 135.38 0.66 10.04 120.82 1.02 30.48 0.94 9.89 10.04 15.85.79.42 1.03 119.47 10.05 106.62 1.06 26.90 1.02 10.04 10.11 13.9954.59 40 1.04 82.11 10.06 73.28 1.10 18.49 1.89 0.35 10.19 60 9.6129.22 1.05 43.95 10.07 39.22 1.14 9.90 12.00 2.40 10.27 5.1512.18 1.06 18.32 10.08 16.35 1.18 4.12 PASS PASS 10.35 2.143.95 1.07 5.95 10.09 5.31 1.22 1.34 8.07 1.58 10.43 0.7020801.00 1.07 1.50Imprecision10.10 1.34 1.27 0.34 8.00 1.60 10.51 0.180.20 1.08 Manufacturer 0.30Specification - Dotted Line 10.11 0.26 1.31 0.07 FAIL PASS 10.59 1 µL0.03Calibration Data -Solid Line0.03 1.09 0.05 10.12 0.04 1.35 0.01 0.08 0.16 10.67 0.0101000.820 0.880 0.940 1.000 1.060 1.120 1.180Manufacturer Specification - InaccuracyCustomer DataUncertainty of MeasurementIn Use At: Pipette Clinic Pty Ltd Coverage factor at confidence level 95 %k=2Level 1, Suite 44Standard Uncertainty (1 µL)0.019 µL48-50 George Street Standard Uncertainty (10 µL)0.021µLParramatta, NSW, 2150 Uncertainty at confidence level 95% ( 1.02 ± 0.06 ) µLTest Method: AS 2162.2-1998, ISO-8655 Uncertainty at confidence level 95% ( 10.04 ± 0.06 ) µLPage 3 of 3M.Balac