microscope components for transmitted light ... - Nikon Instruments

Specifications and equipment are subject to change without any notice or 

obligation on the part of the manufacturer. June 2007. ©1997-07 NIKON CORPORATION 

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Printed in Japan (0706-03)T Code No. 2CE-MUZH-3 This brochure is printed on recycled paper made from 40% used material. 

En 

MICROSCOPE COMPONENTS 

FOR TRANSMITTED 

LIGHT APPLICATIONS

2 

Contents 

System Diagram .............................. 

CFI 60 Objectives 

CFI Achromat Series 

for Brightfield/Phase Contrast (DL)............ 

CFI Achromat 4x 

CFI Achromat 10x / CFI Achromat DL 10x 

CFI Achromat LWD 20x / CFI Achromat LWD DL 20x 

CFI Achromat 40x / CFI Achromat DL 40x 


CFI Achromat 100x Oil / CFI Achromat DL 100x Oil 

CFI Achromat LWD DL 20x F 

CFI Achromat LWD 40x C / CFI Achromat LWD DL 40x C 

CFI Plan Achromat Series 

for Brightfield/Phase Contrast (DL) ............ 

CFI Plan Achromat UW 1x 


CFI Plan Achromat 4x 

CFI Plan Achromat 10x / CFI Plan Achromat DL 10x 



CFI Plan Achromat 40x NCG 

CFI Plan Achromat 50x Oil 

CFI Plan Achromat 100x Oil / CFI Plan Achromat DL 100x Oil 


CFI Plan Fluor Series 

for Brightfield/Phase Contrast (DLL) .......... 

CFI Plan Fluor 4x 

CFI Plan Fluor 10x / CFI Plan Fluor DLL 10x 



CFI Plan Fluor 40x Oil 

CFI Plan Fluor 60x C 

CFI Plan Fluor 100x Oil / CFI Plan Fluor DLL 100x Oil 

CFI Plan Fluor 100x Oil with iris diaphragm 

CFI Plan Fluor Series for Phase Contrast (DL).... 

CFI Plan Fluor DL 4x 


CFI Plan Fluor ELWD Series 

for Brightfield/Phase Contrast (DM/DLL) ........ 

CFI Plan Fluor ELWD 20x C / CFI Plan Fluor ELWD DM 20x C 

CFI Plan Fluor ELWD 40x C / CFI Plan Fluor ELWD DM 40x C 

CFI Plan Fluor ELWD 60x C / CFI Plan Fluor ELWD DLL 60x C 

Multi-Immersion Objective for Brightfield ..... 

CFI Plan Fluor 20x MI 

CFI Plan Apochromat Series 

for Brightfield/Phase Contrast (DM) ......... 11~12 

CFI Plan Apochromat 2x 



CFI Plan Apochromat 20x / CFI Plan Apochromat DM 20x 

CFI Plan Apochromat 40x C / CFI Plan Apochromat DM 40x C 

CFI Plan Apochromat 40x Oil / CFI Plan Apochromat DM 40x Oil 

CFI Plan Apochromat 60x C / CFI Plan Apochromat DM 60x C 


CFI Plan Apochromat 60x WI 


CFI Plan Apochromat 100x NCG Oil 

CFI S Fluor Series.......................................... 13 

CFI S Fluor 10x 


CFI S Fluor 40x C 

CFI S Fluor 40x Oil 


Water Dipping Series.................................... 14 

CFI Fluor 10x W 



3 

4~5 

6~7 

8~9 

9 

10 

10 

CFI HMC Series 

for Hoffmann Modulation Contrast ® .............. 

CFI HMC 10x 

CFI HMC 20x F 

CFI HMC LWD 40x C 

15 

2nd Objective Lens Unit ............................... 15 

Markings on CFI60 Objectives........................ 16 

17 

Condensers.................................... 

C-C Abbe Condenser, Dry Type 

C-C Achromat Condenser 

Achromat Swing-out Condenser 

C-C Achromat /Aplanat Condenser 

LWD Achromat Condenser 

C-C Low Power Condenser 

Dark Field Condenser, Oil 

Dark Field Condenser, Dry 

C-CEL Expander Lens (for darkfield) 

Illumination Systems ................ 18~19 

Lamphouse HMX-3B 

Lamphouse HMX-4B, built-in back mirror 

Lamphouse HMX 

Mercury Lamp Socket S 100W for HMX-3B 

Halogen Lamp Socket 100W/HMX 

C-FC Epi-Fl Collector Lens 

Quartz Epi-Fl Collector Lens 

Collector Lens/QH100W 

C-LA HMX Lamphouse Adapter 

HMX Connecting Adapter SB-X1 (Bayonet to Screw) 

HMX Connecting Adapter SB-X2 (Screw to Bayonet) 

Eyepiece Tubes .............................. 19 

Y-TB Binocular Tube 

Y-TE Ergonomic Binocular Tube 

Y-TF Trinocular Tube “F” UW 

Y-TT Trinocular Tube “T” UW 

Intermediate Tubes ....................... 20 

Y-FL Epi-Fl Attachment 

Y-IDP Double Port 

Eyepieces...................................... 20 

CFI 10x with diopter adjustment 

CFI 10x M Photomask with diopter adjustment 

CFI 12.5x with diopter adjustment 


Nosepiece ...................................... 21 

V-NA Motorized Sextuple Nosepiece 

V-ND MG-DIC Intelligent Sextuple Nosepiece 

C-N Sextuple Nosepiece 

C-ND Sextuple DIC Nosepiece 

CCTV Camera Adapters ............. 22~23 

V-T Photo Adapter 

Y-T TV Tube for C-Mount Adapter A 

C-Mount TV Adapter A 

Relay Lens 1x I 

C-Mount TV Adapter 

ENG-Mount TV Adapter 

Zoom Lens 

C-Mount Zooming Adapter 

ENG-Mount Zooming Adapter 

C-Mount TV Adapter 0.45x 


ENG-Mount TV Adapter 0.45x (for Trinocular Tube “F”, “T”) 

ENG-Mount TV Adapter 0.6x (for Trinocular Tube “F”, “T”) 

C-Mount TV Adapter VM 2.5x 

C-Mount TV Adapter VM 4x 

System Diagram 

Mercury Lamp 

Socket S 100W 

Halogen Lamp 

Socket 100W/HMX 

C-C Abbe Condenser 

(Dry) 

ENG-Mount CCTV Camera C-Mount CCTV Camera 

ENG-Mount 

TV Adapter 

ENG-Mount 

TV 

Adapter*1*2 

0.45X, 0.6X 

Y-T TV Tube 

for C-Mount 

Adapter A 



with Built-in Back Mirror 

Lamphouse HMX 

ENG- 

Mount 

Zooming 

Adapter 

Relay 

Lens 1X I 


C-Mount 

Zooming 

Adapter 

Zoom Lens 

Illumination Systems 


HMX Connecting Adapter SB-X1 

(Bayonet to Screw Mount) 


(Screw to Bayonet Mount) 


Quartz Epi-Fl Collector Lens 

Collector Lens/QH 100W 

C-C Achromat Condenser Achromat Swing-out 

Condenser 

C-Mount TV 

Adapter 

Relay Lens 

1X I 

C-C Achromat/Aplanat 

Condenser 

C-Mount 

TV Adapter 

0.45X, 0.6X 

Condensers 

C-Mount 

TV 

Adapter A 

V-NA 

Motorized 

Sextuple Nosepiece 

LWD Achromat 

Condenser 

C-Mount 

TV Adapter 

VM2.5X 

CFI 10X 

with Diopter Adjustment 

Eyepiece Tubes 

Y-TT Trinocular Tube "T" UW Y-TF Trinocular Tube "F" UW Y-TB Binocular Tube Y-TE Ergonomic 

Binocular Tube 

C-Mount TV 

Adapter A 

C-Mount 

TV Adapter 

VM4X 

CFI 10XM 

Photomask with 

Diopter Adjustment 


V-ND 

MG-DIC Intelligent 

Sextuple Nosepiece 

C-C Low Power 

Condenser 

C-Mount 

TV Adapter A 

CFI 12.5X 



Intermediate Tubes 

CCTV Camera Adapters 

*1: Use the dedicated 0.45X adapter for the double port sub-port. 

The 0.6X adapter cannot be used with the double port sub-port. 

*2: ENG-mount CCTV adapter 0.45x is a 38-bayonet mount type; 

other ENG-mount CCTV adapters are 48-bayonet mount types. 

C-N 

Sextuple 

Nosepiece 

CFI60 Objectives 

Darkfield Condenser (Oil) 

C-CEL Expander Lens 

Eyepieces 

CFI 15X 



Nosepieces 

C-ND DIC 

Nosepiece 

Darkfield Condenser 

(Dry) 

3

4 

CFI60 OBJECTIVES 

CFI Achromat Series for Brightfield/Phase Contrast (DL) 

Correction for chromatic aberration in these objectives have been dramatically improved and are at the same level 

as the CFI Plan Achromat Series. These objectives were also corrected for spherical aberration and coma and 

image flatness across the 22mm field of view has been drastically improved. 

The result: truly exceptional quality for this class of objectives. 

The DL phase contrast objectives in this series have more contrast than the DLL objectives for enhanced contrast 

with unstained specimens. 

4.5 

19.9 10 

3.7 3 8 

34.5 

10 5 

30˚ 

45˚ 

35˚ 

C1 

ø27 

M25 ~0.75 

4 X /0.10 

/- WD 30 

ø25 


ø27 

M25 ~0.75 

40X /0.65 

WD 0.65 

/0.17 

ø7 

ø13 

ø17 

ø20 

ø25 

60.06 

60.06 


CFI Achromat DL 40x 

4.5 

2.3 5 36.5 

10 

5 

3 8 

34.5 

10 

4.1 

45˚ 

45˚ 

ø27 

M25 ~0.75 

/- WD 6.1 

45˚ 10X /0.25 

ø10 

ø16 

ø25 


CFI Achromat DL 10x 

35˚ 

ø27 

M25 ~0.75 

60X /0.80 

/0.17 WD 0.3 

ø7 

ø13 

ø17 

ø20 

ø25 


60.06 

60.06 

5 

5 8 

34.5 

10 

2.2 

4.5 

4.1 6 36 

10 

45˚ 

45˚ 

ø27 

M25 ~0.75 

20X /0.40 

/0.17 WD 3.8 

ø8 

ø14.3 

ø17 

ø25 

60.06 

CFI Achromat LWD 20x 

CFI Achromat LWD DL 20x 

45˚ 

20˚ 

ø27 

M25 ~0.75 

00X / 1.25 oil 

/0.17 WD 0.18 

ø5 

ø13 

ø16.6 

ø20 

ø25 

60.06 

CFI Achromat 100x Oil 

CFI Achromat DL 100x Oil 

60.41 

ø25 

ø17 

ø14.3 

ø8.1 

D 20X /0.4 

Ph1 DL 

/1.2 WD 3.0 

M25 ~0.75 

ø27 

45˚ 

45˚ 

4.2 

6 

36 

10 

4.5 

CFI Achromat LWD DL 20x F 

60.41 

ø28.2 

ø25.5 

ø20 

ø12.2 

D 40X /0.55 

WD 2.1 

/0–2 

2 1.6 1.2 0.8 0.4 0 

M25 ~0.75 

ø29 

ø32 

ø34 

35˚ 

4.5 

3 

24.6 25 

5 

CFI Achromat LWD 40x C 

CFI Achromat LWD DL 40x C 

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring 

CFI Achromat 4x 0.10 30.0 50.0 27.50 72 

CFI Achromat 10x 0.25 6.1 20.0 4.40 85 

CFI Achromat DL 10x 0.25 6.1 20.0 4.40 85 ph1 

CFI Achromat LWD 20x 0.40 3.8 10.0 1.72 90 

CFI Achromat LWD DL 20x 0.40 3.8 10.0 1.72 90 ph1 

CFI Achromat 40x 0.65 0.65 5.0 0.65 130 Spring-loaded 

CFI Achromat DL 40x 0.65 0.65 5.0 0.65 130 Spring-loaded ph2 

CFI Achromat 60x 0.80 0.3 3.3 0.43 130 Spring-loaded 

CFI Achromat 100x Oil 1.25 0.18 2.0 0.27 130 Spring-loaded 

CFI Achromat DL 100x Oil 1.25 0.18 2.0 0.27 130 Spring-loaded ph3 

CFI Achromat LWD DL 20x F 0.40 3.0 10.0 1.72 90 Cover glass thickness 1.2mm ph1 

CFI Achromat LWD 40x C 0.55 2.7–1.7 5.0 0.91 195 Cover glass correction 0.2mm 

CFI Achromat LWD DL 40x C 0.55 2.7–1.7 5.0 0.91 195 Cover glass correction 0.2mm ph2 

5

6 


CFI Plan Achromat Series for Brightfield/Phase Contrast (DL) 

These objectives provide incredible image flatness over the entire 25mm field of view, with chromatic aberration 

corrected throughout the entire visible spectrum. These objectives are suitable not only for routine laboratory work 

but for photomicrography. 

The DL phase contrast objectives in this series have more contrast than the DLL objectives for enhanced contrast 

with unstained specimens. 

10.7 46 

5 

2.4 37 

10 5 

ø30 

M25 ~0.75 

Plan UW 

1X /0.04 

/-WD 3.2 

ø31 


45˚ 

45˚ 

ø27 

M25 ~0.75 

Plan 

10X /0.25 

/-WD 10.5 

ø12 

ø19 

ø25 

60.06 


CFI Plan Achromat DL 10x 

60.06 

52.4 5 

4.6 5 4.5 

34.5 

10 4.5 

5 

ø30 

M25 ~0.75 

Plan UW 

2X /0.06 

/-WD 7.5 

ø20 


45˚ 

45˚ 

ø27 

M25 ~0.75 

Plan 

20 X /0.40 

/ 0.17 WD 1.3 

ø7 

ø13 

ø17 

ø20 

ø25 

60.06 

60.06 



19.9 10 4.5 

3.3 4 7.5 

34.5 

10 5 

Plan 

ø27 

M25 ~0.75 

4X/0. 

/-WD 3 

ø25 


45˚ 

30˚ 

ø27 

M25 ~0.75 

Plan 

40 X /0.65 

/ 0.17 WD0.57 

ø6 

ø13 

ø17 

ø20 

ø25 

60.06 

60.06 



5.3 

50.5 

5.5 3.5 

3 

ø30 

M25 ~0.75 

Plan 

40 X /0.65 N 

0 WD 0.48 

/ 

45˚ 

45˚ 

ø7.5 

ø14 

ø18.8 

60 


2.2 4 9 44.5 

5 

45˚ 

ø27 

M25 ~0.75 

Plan 

/ 0.17 WD 0.17 

22.5˚ 100 X /1.25 

ø5.3 

ø13 

ø16.8 

ø20 

ø25 

60.06 


CFI Plan Achromat DL 100x Oil 

4.5 4.3 51.2 

5.3 

5 

51.2 

2.2 6.3 

45˚ 

ø30 

M25 ~0.75 

Plan 

50 X /0.90 oil 

WD 0.40 

30˚ /- 

ø5.5 

ø14 

ø18.8 

60.06 (NCG) 


2 

45˚ 

20˚ 

ø30 

M25 ~0.75 

Plan 

100 X /0.90 N 

/ 0 WD 0.26 

ø5.5 

ø14 

ø18.8 

60 



CFI Plan Achromat UW 1x 0.04 3.2 200.0 171.88 180 

CFI Plan Achromat UW 2x 0.06 7.5 100.0 76.39 110 

CFI Plan Achromat 4x 0.10 30.0 50.0 27.50 65 


CFI Plan Achromat DL 10x 0.25 10.5 20.0 4.40 85 ph1 


CFI Plan Achromat DL 20x 0.40 1.3 20.0 1.72 85 ph1 

CFI Plan Achromat 40x 0.65 0.57 5.0 0.65 115 Spring-loaded 

CFI Plan Achromat DL 40x 0.65 0.57 5.0 0.65 115 Spring-loaded ph2 

CFI Plan Achromat 40x NCG 0.65 0.48 5.0 0.65 160 Spring-loaded No cover grass 

CFI Plan Achromat 50x Oil 0.90 0.4 4.0 0.52 160 Spring-loaded 

CFI Plan Achromat 100x Oil 1.25 0.17 3.3 0.52 160 Spring-loaded 

CFI Plan Achromat DL 100x Oil 1.25 0.17 3.3 0.52 160 Spring-loaded ph3 

CFI Plan Achromat 100x NCG 0.90 0.26 2.0 0.18 160 Spring-loaded No cover grass 

7

8 


CFI Plan Fluor Series for Brightfield/Phase Contrast (DLL) 

Featuring an extra-high transmission 

rate, especially in the ultraviolet 

wavelength, and flatness of field 

comparable to the CFI Plan Achromat 

series, these objectives are designed 

for fluorescence observation and 

photomicrography. Because of this 

improvement in quality, these 

objectives can function as multipurpose 

objectives for brightfield, 

fluorescence, polarizing, and DIC 

observations. 

The DLL (low contrast) phase 

objectives for this series can also be 

used as universal objectives for 

phase contrast, fluorescence, DIC, 

and brightfield techniques. 

5.3 

54 

3.8 

ø30 

M25 ~0.75 

Plan Fluor 

20X / 0.50 

DIC M 

/0.17 WD 2.1 

ø12 

ø17 


CFI Plan Fluor DLL 20x 

45˚ 

5.3 

57.2 

2 

5.3 

42.8 

5.5 

ø30 

M25 ~0.75 

Plan Fluor 

4X / 0.13 

/ - WD 17.1 

ø20 


ø30 

M25 ~0.75 

Plan Fluor 

40X / 0.75 

DIC M 

/0.17 WD 0.72 

ø10 




CFI Plan Fluor 4x 0.13 17.1 50.0 16.27 110 


CFI Plan Fluor DLL 10x 0.30 16.0 20.0 3.06 120 ph1 


CFI Plan Fluor DLL 20x 0.50 2.1 10.0 1.10 175 ph1 

CFI Plan Fluor 40x 0.75 0.72 5.0 0.49 210 Spring-loaded 

CFI Plan Fluor DLL 40x 0.75 0.72 5.0 0.49 210 Spring-loaded ph2 

CFI Plan Fluor 40x Oil 1.30 0.2 5.0 0.25 230 Spring-loaded Stopper 

CFI Plan Fluor 60x C 0.85 0.3 3.3 0.38 265 Spring-loaded Cover glass correction 0.11–0.23mm 

CFI Plan Fluor 100x Oil 1.30 0.2 2.0 0.25 250 Spring-loaded Stopper 

CFI Plan Fluor DLL 100x Oil 1.30 0.2 2.0 0.25 250 Spring-loaded Stopper ph3 

CFI Plan Fluor 100x Oil with iris diaphragm 0.5–1.3 0.2 2.0 0.25 285 Spring-loaded With iris diaphragm 

45˚ 

45˚ 

45˚ 

5.3 

12 

40 

4 

3.7 

5.3 

43.9 

45˚ 

2.7 

ø30 

M25 ~0.75 

Plan Fluor 

10X / 0.30 

DIC L @ 

/0.17 WD 16.0 

ø18 



ø33.5 

ø30 

M25 ~0.75 

Plan Fluor 

DIC H 

/0.17 WD 0.2 

23˚ 40X / 1.30 oil 

ø8 

ø22 

ø25.8 


45˚ 

1.7 

5.3 

10.8 11 

32 

3.9 

60.41 

ø34.5 

ø30 

M25 ~0.75 

Plan Fluor 

60X / 0.85 

DIC M 

/0.11-0.23 WD 0.30 

11 14 17 20 23 

ø8.5 

ø18 

ø22 

ø33 

CFI Plan Fluor 60x C 

Plan Fluor 

4X / 0.13 

PhL DL 

/1.2 WD 16.4 

M25 ~0.75 

ø30 


30˚ 

45˚ 

30˚ 

5 

42.8 

5.3 

5.3 

3.8 4.5 

51.5 

ø30 

Plan Fluor 

100X/1.30 oil 

DIC H 

/0.17 WD 0.20 

ø5.8 

ø17 

ø21 


CFI Plan Fluor DLL 100x Oil 

CFI Plan Fluor Series for Phase Contrast (DL) 

60.41 

1.2 

Plan Fluor 

10X / 0.30 

Ph1 DL 

/1.2 WD 15.2 

M25 ~0.75 

ø30 


CFI Plan Fluor DL 4x 0.13 16.4 50.0 16.27 105 Cover glass thickness 1.2mm phL 

CFI Plan Fluor DL 10x 0.30 15.2 20.0 3.06 120 Cover glass thickness 1.2mm ph1 

45˚ 


20˚ 

45˚ 

6 

44 

5.3 

6.2 

2.6 

5.3 

34 

2.9 6.5 7.5 

17˚ 

ø33.4 

ø32 

ø30 

M25 ~0.75 

Plan Fluor 

100X/0.5-1.3 oil 

/0.17 WD 0.2 

1.3 0.7 

ø5.3 

ø19.8 

ø23.6 

ø32 

C2.5 

8˚ 

CFI Plan Fluor 100x Oil with 

iris diaphragm 

These objectives have more contrast than the DLL objectives for enhanced contrast with tissue culture 

specimens. These objectives are designed for use on inverted microscopes and therefore their markings are 

upside-down for easy reading. 

9

10 


CFI Plan Fluor ELWD Series for Brightfield/Phase Contrast (DM/DLL) 

Offering superb flatness of field, high UV transmission rates, and high Numerical Apertures with extra long working distances, these objectives are 

well suited for fluorescence observations, especially with inverted microscopes. Because of their superior optical design, CFI Plan Fluor ELWD DM 

objectives can be used universally for all other observation techniques, including brightfield, fluorescence, phase contrast, and Nomarski DIC. 

The DM phase contrast objectives have higher contrast to enhance the image of very thin, highly detailed specimens with low phase gradients. 

The DLL objectives have less contrast for thicker, more contrasty specimens. 

60.41 

1.2 

Standard 

thickness 

ø32.5 

ø28.7 

ø25.5 

ø16.4 

Plan Fluor 

LWD 20X/0.45 

/ 0-2 WD 7.4 

2 1.6 1.2 0.8 0.4 0 

ø29 

ø32 

ø34 

7˚ 

25˚ 

22.2 1.8 

1.8 

Objective N.A. W.D. Focal length Physical depth Weight Remarks 

(magnification) (mm) (mm) of focus (µm) (g) 

CFI Plan Fluor 20x MI 0.75 0.35–0.33 10.0 0.49 300 

11.5 

13 

5 

CFI Plan Fluor ELWD 20x C 

CFI Plan Fluor ELWD DM 20x C 

5˚ 

60.41 

ø32.5 

ø29 

ø27.5 

ø13.3 

Plan Fluor 

LWD 40X/0.60 

/0-2 WD 3.7 - 2.7 

2 1.6 1.2 0.8 0.4 0 

M25 ~0.75 

ø29 

ø32 

ø34 


CFI Plan Fluor ELWD 20x C 0.45 8.1–7.0 10.0 1.36 195 Cover glass correction 0–2mm 

CFI Plan Fluor ELWD DM 20x C 0.45 8.1–7.0 10.0 1.36 195 Cover glass correction 0–2mm ph1 

CFI Plan Fluor ELWD 40x C 0.60 3.7–2.7 5.0 0.76 195 Cover glass correction 0–2mm 

CFI Plan Fluor ELWD DM 40x C 0.60 3.7–2.7 5.0 0.76 195 Cover glass correction 0–2mm ph2 

CFI Plan Fluor ELWD 60x C 0.70 2.1–1.5 3.3 0.56 210 Cover glass correction 0.5–1.5mm 

CFI Plan Fluor ELWD DLL 60x C 0.70 2.1–1.5 3.3 0.56 210 Cover glass correction 0.5–1.5mm ph2 

5 

7˚ 

20˚ 

Multi-immersion 

Oil-glycerine-water 

26.5 2.5 

2.5 

11.5 

13 

5 


CFI Plan Fluor ELWD DM 40x C 

Multi-Immersion Objective for Brightfield 

This multi-purpose immersion objective has a higher numerical aperture and 

superior contrast. The use of immersion media helps eliminate surface 

reflections and provides an image with higher contrast. It is an excellent 

objective for fluorescence, brightfield, and DIC observation techniques. 

This unique objective has a correction collar that allows it to be used with 

immersion oil, glycerin, and water (with and without a cover glass). 

60.41 

10.4 19 12.5 14 5.3 

3.7 

ø32.5 

ø26.3 

ø10 

Plan Fluor 

WD 60X/0.70 

0.5-1.5 WD 2.1-1.5 

1.5 1.2 1 0.8 0.5 

M25 ~0.75 

ø29 

ø33 

ø34 

ø36 

ø34.5 

ø30.6 

M25 ~0.75 

0.17 0 

Oil G W 

Plan Fluor 

20X / 0.75 Mlmm 

/ - WD 0.35 

ø8 

ø28 

ø33 

7.5˚ 

9˚ 

22.5 

6 

33 

11.5 

13 

5 


CFI Plan Fluor ELWD DLL 60x C 

CFI Plan Fluor 20x MI 

40˚ 

8˚ 

3˚ 

30˚ 

CFI Plan Apochromat Series for Brightfield/Phase Contrast (DM) 

These objectives feature longer working distances with high Numerical Apertures and is designed to correct all 

optical aberrations throughout the visible spectrum from violet to red from center to edges across the entire 25mm 

field of view. Superior image flatness and color reproduction, plus resolving power at the theoretical limit of today's 

optical technology are also featured. 

The DM phase contrast Plan Apochromats have higher contrast to enhance images of very thin, highly detailed 

specimens with low phase gradients. 

5.3 

12 

5.9 2.5 38.5 

5.3 

51.4 

45˚ 

45˚ 

ø30 

M25 ~0.75 

Plan Apo 

2X / 0.1 

/ - WD 8.5 

ø19 

WD=8.5 

CG=0.17 


30˚ 

ø33.5 

ø30 

M25 ~0.75 

Plan Apo 

20X / 0.75 

DIC M 

/0.17 WD 1.0 

ø13.5 

ø22 

ø26 

WD=1.0 

CG=0.17 

60.06 

60.06 


CFI Plan Apochromat DM 20x 

1.7 

4.2 10.8 11 

32 5.3 

5.3 

44.2 

30˚ 

30 

ø30 

M25 ~0.75 

Plan Apo 

4X / 0.2 

/ - WD 15.7 

ø18 

WD=15.7 

CG=0.17 


30˚ 

3˚ 

ø34.5 

ø30 

M25 ~0.75 

Plan Apo 

40X / 0.95 

DIC M 

/0.11-0.23 WD 0.14 

11 14 17 20 23 

ø7 

ø18 

ø22 

ø33 

WD=0.14 

CG=0.17 

60.06 

60.06 

CFI Plan Apochromat 40x C 

CFI Plan Apochromat DM 40x C 

5.3 

55.9 

5 

12 

4.7 4.5 38.5 

ø30 

M25 ~0.75 

Plan Apo 

DIC L 

/0.17 WD 4.0 

45˚ 10X / 0.45 

ø15 

WD=4.0 

CG=0.17 


45˚ 

30˚ 

5˚ 

ø32 

ø30 

ø25 

Plan Apo 

40X / 1.0 oil 

DIC H 

/0.17 WD 0.16 

ø8 

ø18.8 

ø22.8 

WD=0.16 (oil) 

CG=0.17 

60.06 

60.06 

CFI Plan Apochromat 40x Oil 

CFI Plan Apochromat DM 40x Oil 

11

12 


1.7 

5.3 

5.3 

32 

11 

10.8 

4.2 

30˚ 

30˚ 

3˚ 

ø34.5 

ø30 

M25 ~0.75 

Plan Apo 

60X /0.95 

DIC M 

0.11-0.23 WD 0.15 

/ 

11 14 17 20 23 

ø7 

ø18 

ø22 

ø33 

WD=0.15 

CG=0.17 

60.06 

CFI Plan Apochromat 60x C 

CFI Plan Apochromat DM 60x C 

59.8 

51.5 

4.5 

3.8 

20˚ 

C2 

ø30 

M25 ~0.75 

Plan Apo 

100 X /1.40 oil 

DIC H 

/ 0.17 WD 0.13 

ø5.8 

ø17 

ø21 

WD=0.13 

60.06 



CFI Plan Apochromat Series for Brightfield/Phase Contrast (DM) 

3.7 4 

40 

12 5.3 

45˚ 

22.5˚ 

8˚ 

ø33.5 

ø30 

M25 ~0.75 

Plan Apo 

60X /1.40 oil 

DIC H 

/ 0.17 WD 0.21 

ø7.2 

ø22 

ø25.8 

CG=0.17 WD=0.21 (oil) 

60.06 



59.8 5.3 

51.5 

4.5 

3.8 

C2 

20˚ 

ø30 

M25 ~0.75 

Plan Apo 

00X /1.40 oilN 

/ 0 WD 0.17 

ø5.8 

ø17 

ø21 

WD=0.17 

60 

CFI Plan Apochromat 100x NCG Oil 

59.7 5.3 

23.5 

11 

11.9 

10.4 

2.9 

30˚ 

7˚ 

5˚ 

30˚ 

ø36.5 

ø35.2 

ø30 

M25 ~0.75 

Plan Apo 

60X /1.20 WI 

/ 0.15-0.18 WD 0.22 

15 16 17 18 

ø7.5 

ø29 

ø33.4 

CFI Plan Apochromat 60x WI 


CFI Plan Apochromat 2x 0.10 8.5 100.0 27.50 180 



CFI Plan Apochromat 20x 0.75 1.0 10.0 0.49 240 Spring-loaded 

CFI Plan Apochromat DM 20x 0.75 1.0 10.0 0.49 240 Spring-loaded ph2 

CFI Plan Apochromat 40x C 0.95 0.14 5.0 0.30 270 Spring-loaded Cover glass correction 0.11–0.23mm 

CFI Plan Apochromat DM 40x C 0.95 0.14 5.0 0.30 270 Spring-loaded Cover glass correction 0.11–0.23mm ph2 

CFI Plan Apochromat 40x Oil 1.0 0.16 5.0 0.42 240 Spring-loaded Stopper 

CFI Plan Apochromat DM 40x Oil 1.0 0.16 5.0 0.42 240 Spring-loaded Stopper ph3 

CFI Plan Apochromat 60x C 0.95 0.15 3.3 0.30 285 Spring-loaded Cover glass correction 0.11–0.23mm 

CFI Plan Apochromat DM 60x C 0.95 0.15 3.3 0.30 285 Spring-loaded Cover glass correction 0.11–0.23mm ph2 



CFI Plan Apochromat 60x WI 1.20 0.22 3.3 0.25 285 Spring-loaded Cover glass correction 0.15–0.18mm 

Water immersion 



CFI Plan Apochromat 100x NCG Oil 1.40 0.17 2.0 0.21 245 Spring-loaded Stopper No cover glass 

CFI S Fluor Series 

These objectives ensure a high transmission rate of ultraviolet wavelengths down to 340 nm for fluorochromes like 

indo-1, fura-2, and fluor-3. Also, these objectives have improved signal-to-noise ratios (S/N) for short wavelengths and 

have high N.A., making the fluorescence images they produce significantly sharper and brighter. 

64 

5.3 

12 

38.5 

8.2 

5 

12 

37 

6.5 

4.2 

ø32 

ø30 

M25 ~0.75 

S Fluor 

10 X / 0.50 

/ 0.17 

ø8 

ø19 

ø24 

WD 0.12 


ø32 

ø30 

M25 ~0.75 

S Fluor 

40 X / 1.30 

/ 0.17 

WD 0.22 

ø8.3 

ø20 

ø23.8 

Oil 


1.2 

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks 

5.3 

64.2 

5.9 41 

12 

64.7 

ø33.5 

ø30 

M25 ~0.75 

S Fluor 

20 X / 0.75 

/ 0.17 

CFI S Fluor 10x 0.50 1.2 10.0 1.10 275 Spring-loaded 

CFI S Fluor 20x 0.75 1.0 10.0 0.49 275 Spring-loaded 

CFI S Fluor 40x C 0.90 0.3 5.0 0.34 285 Spring-loaded Cover glass correction 0.11–0.23mm 

CFI S Fluor 40x Oil 1.30 0.22 5.0 0.25 275 Spring-loaded 

CFI S Fluor 100x Oil 1.30 0.2 2.0 0.25 285 Spring-loaded 

5 

32 

7.5 

7.5 

9.8 

2.9 

ø9 

ø22 

ø25 

WD 1.0 


ø32 

ø30 

M25 ~0.75 

S Fluor 

100 X /0.5-1.3 oil 

/ 0.17 WD 0.2 

ø6 

ø17 

ø24 

ø29.5 

1.3 0.7 


1 

5 

27 

3.1 8.5 10.5 10.5 

ø34.5 

ø33 

ø30 

M25 ~0.75 

Fluor 40 X / 0.9 

/ 0.11-0.23 WD 0.3 

11 14 17 20 23 

ø7.5 

ø18 

ø26 

ø33 

CFI S Fluor 40x C 

13

14 

Water Dipping Series CFI HMC ® Series for Hoffmann Modulation Contrast ® 


These objectives (no cover glass) are designed with a narrow top and a 2mm working distance to allow 

microelectrodes to be inserted into the specimen at 45°. They use ceramic and glass nose cones to isolate them 

thermally and electronically. Since they are FLUOR lenses, they are designed for fluorescence and have very high 

transmission in the UV and IR wavelengths with a high signal-to-noise ratio for superior images. 

5 

58.5 

32.7 

1.5 12 3.8 8.5 

55˚ 

45˚ 

ø29 

M25 ~0.75 

Fluor 

10X / 0.30 W 

DIC L 

/ 0 WD 2.0 

ø3.5 

ø5 

ø20.5 

ø24 

ø26 


48˚ 

2 (Water dipping) 

60.5 

5 

58.5 


CFI Fluor 10x W 0.30 2.0 20.0 4.06 210 Water dipping 



32.7 

4.5 5 5 11.3 

50˚ 

45˚ 

ø29 

M25 ~0.75 

Fluor 

40X / 0.80 W 

DIC M 

/ 0 WD 2.0 

ø4 

ø11.5 

ø19 

ø23 

ø25.5 


45˚ 


60.5 

5 

58.5 

32.7 

1 5.5 8 11.3 

40˚ 

30˚ 

ø29 

M25 ~0.75 

Fluor 

60X / 1.00 W 

DIC H 

/ 0 WD 2.0 

ø19.2 

ø23 

ø25.5 

ø5.5 


33.7˚ 


60.5 

These objectives have been completely redesigned by Nikon, allowing the contrast direction to be changed using 

a modulator inside the objective. That direction, once set, is maintained over the entire magnification range from 

10X to 40X. 

Note: Hoffman Modulation Contrast and HMC are registered trademarks of Modulation Optics Inc. 

60.41 

ø25 

ø13.3 

ø10 

10X / 0.25 

MC1 HMC 

45˚ 

45˚ 

/ 1.2 WD 5.2 

M25 ~0.75 

ø27 

ø27.8 

CFI HMC 10x 

2nd Objective Lens Unit 

10 13 

28.5 4.5 2.5 

4.5 

This unit is attached to infinity objectives when neither a 

binocular or trinocular eyepiece tube is used. 

• Infinity Tube Lens Unit for CFI objectives (chromatic aberrationfree 

infinity corrected objectives). 

• Focusing distance: 200mm (7.87 in.) 

• To obtain the optimal objective performance, keep the distance 

between the tube lens and the objective’s shoulder within 

100–200 mm (3.84–7.87 in.). 

60.41 

ø25 

ø16.5 

ø15 

ø8 

45˚ 

45˚ 

D 20X / 0.4 

/ 1.2 WD 3.0 

MC2 HMC 

M25 ~0.75 

ø27 

ø27.8 

CFI HMC 20x F 


CFI HMC 10x 0.25 5.2 20.0 4.40 130 Cover glass thickness 1.2mm 

CFI HMC 20x F 0.40 3.0 10.0 1.72 130 Cover glass thickness 1.2mm 

CFI HMC LWD 40x C 0.55 2.7–1.7 5.0 0.91 210 Cover glass correction0–2mm 

4.8 

10 13 

28.5 

4.5 

28 

22.5 

M38 ~0.5 

5 

60.41 

ø36f7 -0.025 

-0.050 

ø33 

Plan Fluor 

100X / 1.30 oil 

DIC H 

/ 0.17 WD 0.20 

ø33.3 

ø32 

ø28.8 

ø25.5 

ø20 

ø12 

WD 40X / 0.55 

/0- 2 WD 2.7-1.7 

MC3 HMC 

1.6 1.2 0.8 0.4 

Approx. 70~170 148 

2nd Objective Lens Unit 

2 

M25 ~0.75 

ø29 

ø32 

ø34 

0 

35˚ 

CFI HMC LWD 40x C 

Image plane 

1 

5.6 

19 

11.5 8 

13 

5 

Objective's shoulder 

15

18 

Illumination Systems 

171 

1 170 

80 

6 

ø45 

1.1 

105 

38.5 

7.5 

3 22 

ø3 

6 

80.5 

136 

12 

139 

24 

61 (Center of lamp) 


77 69.5 

34 

2 

ø42 

4 

ø56 

47 

68 

26 102 

2 

10 

148 

80 

20 

Lamphouse HMX 

ø33 

Center 

of 

lamp 


109 

67.8 

10.3 0.6 

(Center of pin) 

137.3 

(Center of filament) 

(Center of lamp) 

30 

6.6 

ø50 

67.8 

20.5 

37.8 

i35.5 ‘39.5 j 

7 

24 5 

171 

1 170 

80 

ø57 

105 

Halogen Lamp Socket 100W/HMX 

ø45 

36 11 

3 

3 21 

ø36 

Center 

of 

lamp 

80.5 

41.5 

24 

61 

168.5 

139 

(Center of lamp) 

Lamphouse HMX-4B, built-in back mirror 

99.5 

81.5 

52.5 

40 

Mercury Lamp Socket S 100W for HMX-3B 

ø45 

36.5 

3 22 

51 

12.5 

Center of 

lamp 

Quartz Epi-Fl Collector Lens Collector Lens/QH100W 

70 8.5 

76.5 

138.5 

ø67 

ø51 

4 

4.6 

50 

12 24.5 13.5 

90˚ 

(Datum clamp face) 

30 8 


ø71 


76 

Eyepiece Tubes 

100 

70 

ø52 

80 

64 

76.5 

60 

Y-TB Binocular Tube 

218.5 

Y-TF Trinocular Tube “F” UW 

Y-TB Binocular Tube F.O.V. 22mm 

3.5 

M50 ~0.75 


28.5 

15 

20.5 


ø71 


(Bayonet to Screw) 

Y-TE Ergonomic Binocular Tube F.O.V. 22mm with tilting angle of 8° to 32° and telescopic mechanism of ±15mm movement 

50 

120 

16.5 

151.5 

66.5 

76 

ø61 

3 

7 

ø55 

4 

M50 ~0.75-6H 

Sectional View 


(Screw to Bayonet) 

Y-TF Trinocular Tube “F” UW Two-way: 100 : 0 or 0 : 100 (observation : photo) F.O.V. 22mm or 25mm with eyepiece reticule orientation adjustment mechanism 

Y-TT Trinocular Tube “T” UW Three-way: 100 : 0, 20 : 80, or 0 : 100 (observation : photo) F.O.V. 22mm or 25mm with eyepiece reticule orientation adjustment mechanism 

58.5 

ø58 

27.1 

8˚ (Min. angle) 

105 (Min. distance) 

50 

Approx. 111 

90.5 

8 63 

ø51.3 

50.5 77 

R32 

64 

(Tilting section) 

R40 

Y-TE Ergonomic Binocular Tube 

135.9 

102.5 

ø52 

100 

70 

5 

i239 j 

Y-TT Trinocular Tube “T” UW 

98 

100 

110 

ø59 

50 

172 

16.5 

3 

73.7 

19

20 


120.6 

55 

20.5 34.5 

264 

248 

80 

80 

322 

353.5 


Eyepieces 

ø30 

ø30 

22.5 

48–56 

CFI 10X / 22 

Type (magnification) F.O.V. Remarks 

CFI 10x with diopter adjustment 22mm 

126.5 

CFI 10x M Photomask with diopter adjustment 22mm With photomask reticule 

CFI 12.5x with diopter adjustment 16mm 

CFI 15x with diopter adjustment 14.5mm 

ø34 


22.5 

42–47 

CFI 12.5X / 16 

ø35 

CFI 12.5x with diopter adjustment 

ø38.8 

ø38.8 

100 

ø30 

(Top View) 

100 

13.5 

45 25 

5 

50.5 

57.5 

(Right-side View) 

55 45 

22.5 

100 0 

ø51 

22 

285.5 

175 


48–56 

CFI 10X / 22M 

ø34 

ø52 

CFI 10x M Photomask with diopter adjustment 

ø30 

22.5 

37–41 

CFI 15X / 14.5 

ø35 

ø38.8 


ø38.8 

Nosepieces 

6 

50 

1 

2 

53 

48 

2 

22.5 

123.2 

V-NA Motorized Sextuple Nosepiece 

50 

53 

51.1 

48 

22.5 

105.8 

C-N Sextuple Nosepiece 

JAPAN 

ø106 

ø121 

3 

V-N JAPAN 

50 

53 

48 

22.5 

123.2 

V-ND MG-DIC Intelligent Sextuple Nosepiece 

50 

53 

48 

22.5 

C-ND Sextuple DIC Nosepiece 

123.2 

ø121 

ø121 

C-ND DIC6 

V-ND JAPAN 

21

22 

CCTV Camera Adapters 

130 

3 

72 

27 

67.3 

40.2 

ø52 

ø38 

ø52 


ø34 

ø30 

44 

31 

Relay Lens 1x I 

ø58.5 

ø54 

JAPAN 

ø23.1 

ø42 

Zoom Lens 

75 

35 

ø12 

10 

9.3 

Image plane 

on eyepiece tube side 

ø51 

ø38 

ø59 

ø38 

76.5 

Y-T TV Tube for C-Mount Adapter A 

ø31 

ø42 

ø46 

7.5 

64.5 

91.8 17.526 

95.8 

45˚ 

27.3 

C-Mount TV Adapter 

ø44 

ø54 

C-mount screw 

45˚ 

11.5 4 

7.5 42.8 17.526 

54.3 

C-Mount Zooming Adapter 

C-mount screw 

41 

25.5 

TV camera imaging plane 

ø50 

ø25.4 

ø38 

11.47 17.526 

C-Mount TV Adapter A 

ø42 

ø47 

ø62 

ø63 

ENG-Mount TV Adapter 

ø44 

ø57 

30˚ 

27.8 11.5 

53.3 

61.3 }0.1 48 

64.3 

48 

12.3 

7.5 

22.8 

ENG-Mount Zooming Adapter 

Focal point 

ø23.1 

ø42 

ø53 

Focal point 

ø23.1 

ø42 

ø53 

17.526 

27.3 4 

30.7 

30.2 27 

4 

100.1 


38 

111.2 

ENG-Mount TV Adapter 0.45x 

(for Trinocular Tube “F”, “T”) 

Focal point 

ø53 

ø38 

42 29 

68 

C-Mount TV Adapter VM 2.5x 

Focal point 

ø23.1 

ø42 

ø53 

Focal point 

ø23.1 

ø42 

ø53 

17.526 

27 4 

30.7 

30.2 27.0 

3.3 

116.8 


48 

122.8 

ENG-Mount TV Adapter 0.6x 

(for Trinocular Tube “F”, “T”) 

Focal point 

ø53 

ø38 

29 

83 

C-Mount TV Adapter VM 4x 

109 

23

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