chemistry journal of moldova

I. Lunga et al./Chem.J.Mold. 2008, 3 (1), 89-93Table 113C NMR spectral data (150 MHz, CD 3OD) of saponins (1-3)Carbon 1 2 31 37.9 37.9 37.92 30.4 30.2 30.03 78.9 79.1 79.14 34.9 35.1 35.05 45.8 45.8 45.96 32.9 32.9 29.57 34.6 34.6 32.98 36.0 36.0 34.79 55.2 55.2 56.110 36.5 36.4 35.711 21.5 21.5 21.312 40.5 40.5 35.713 41.8 41.8 47.214 57.5 57.5 57.315 32.3 32.3 32.916 82.1 82.2 147.017 63.7 63.7 156.418 16.7 16.7 15.719 12.5 12.6 12.220 42.6 42.6 199.321 14.2 14.2 26.822 110.5 110.523 32.2 32.124 29.1 29.125 31.2 31.226 67.7 67.727 16.7 16.71' 103,5 103,2 102.92' 72,9 78,1 72.93' 80,7 82.5 75.34' 70,5 71,7 80.25' 75,6 75,4 77.86' 60,3 60,1 60.41'' 105,0 104,9 104.52'' 75,5 75,5 84.73'' 78,3 78,0 77.84'' 71,2 71,2 71.45'' 78,9 78,9 78.66'' 63,1 62,9 62.51''' 105,1 105.12''' 75,5 75.53''' 78,3 78.24''' 71,7 71.35''' 78,1 78.96''' 63,1 63.1ExperimentalThe seeds of Hyoscyamus niger L. has been collected in the scientific research field of Institute of ScientificResearches and Technological Constructions for Tobacco and Tobacco Products of Moldova in November 2001 year.The voucher specimen has been deposited in Laboratory of selection under direction of Doctor in Biology KalkeiE.D.Dry powdered seeds (500 g) were extracted three times in 50°C with n-buthanol saturated with water.The received n-butanol-water extract was evaporated and then purified with chloroform. After purification it wascrystallised in acetone. The residue was dried in vacuum at 40°C and the sum of steroidal saponins has been obtainedas yellow powder in 3.7% yield. 3g of extract has been chromatographied on silica gel column (30-500mm, 60-100m,91