Proteomics Process Flow Chart

PROTEOMICS PROCESS FLOW1.1 – Section 1.1Proteomics Process Flow ChartExtra-Cellular ProteinSample/CultureIntra-Cellular ProteinRemove CellsLyse CellsAcroPrep Filter Platesfor Cell Separation/RemovalAntibodyPurificationIonExchangeAcroPrep Filter Plateswith Mustang ® Q & SMembraneProtein MixtureConcentrate SampleAlbumin Depletion/Abundant Protein RemovalPre-FractionationRemove DebrisAcroPrep Filter Platesfor Lysate ClearingEnchant Albumin Depletion KitsEnchant Multi-ProteinAffinity Separation KitsBioSepra ® ResinsDesalting/ConcentrationAssaysAcroWell Filter PlatesWith BioTrace PVDF &NT MembraneChargeBioSepra Resins,AcroPrep Filter Plates withMustang Q & S MembranesSizeAcroPrep Filter Plateswith UF MembraneCentrifugal DevicesMinimate Centramate TFF SystemsBioSepra ResinsLC SeparationGel SeparationWestern BlottingCollect Fractions& Buffer ExchangeDestaining + In-Gel DigestionProteinSequencingDetectionAcroPrep Filter Plateswith UF MembraneAcroPrep Filter Plateswith GHP MembraneFluoroTrans ®MembranesFluoroTrans WMembranesCentrifugal DevicesMinimate TFF SystemsNanosep ® Centrifugal Deviceswith GHP MembraneBioTrace PVDF & NTMembranesBioSepra ResinsDesalting/RebufferConcentrationDesalting/ConcentrationAcroPrep Filter Plateswith UF MembraneCentrifugal DevicesAssayMS-AnalysisCrystallographyMinimate TFF SystemsBioSepra ResinsProtein Detectionwww.pall.com 1

PRODUCT SELECTION1.2 – Section 1.2Application GuideChromatography ProductsSample PreparationUltrogel ® AcA, Trisacryl ® GF Size Exclusion • • •Chromatography ResinsCeramic HyperD ® Ion Exchange Chromatography ResinsTrisacryl, HyperD, HyperCel Affinity Chromatography Resins • •SDR HyperD Solvent-Detergent Removal ResinsMEP HyperCel Hydrophobic Charge InductionChromatography ResinsMustang ® Ion Exchange Membrane DevicesOmega Ultrafiltration Membrane for Size Exclusion • • • •Multi-Well Filter PlatesAcroPrep Plates with Hydrophilic Filtration Membranes • • • •AcroPrep Plates with Ion Exchange MembranesAcroPrep Plates with Prefilters•••† • †† †AcroPrep Plates with Ultrafiltration Membranes for Size Exclusion • • • •AcroPrep Plates with Glass FiberAcroWell Plates with Binding MembranesCentrifugal Devices/Spin FiltersAbundant Protein RemovalProtein FractionationProtein ConcentrationMicrofiltration (MF) Centrifugal Devices • • • †Protein DesaltingUltrafiltration (UF) Centrifugal Devices † •†• • •Buffer Exchange† In combination with appropriate chromatography resin.2

PRODUCT SELECTION1.2 – Section 1.2Application Guide (continued)Protein Purification KitsEnchant Kit for Albumin DepletionAbundant Protein RemovalProtein FractionationProtein ConcentrationSample Preparation•Protein DesaltingBuffer ExchangeEnchant Multi-Protein Affinity Separation Kit • •Tangential Flow Filtration ProductsMinimate TFF Capsules • • • •Ultrasette Lab TFF Devices • • • •LV Centramate Lab TFF Systems • • • •Centramate Lab TFF Systems • • • •Prefiltration/ClarificationAcrodisc ® Syringe Filters with PrefiltersVacuCap ® Vacuum Filtration DevicesDetection ProductsFluoroTrans ® PVDF MembranesBioTrace PVDF MembraneBioTrace NT Nitrocellulose MembraneUltraBind Affinity MembraneImmunodyne ® ABC MembraneArraysProtein Chip System Based on Arrays† In combination with appropriate chromatography resin.4

PRODUCT SELECTION1.2 – Section 1.2Detergent andImpurity RemovalFiltration, Clarification,and Lysate ClearingProtein Purification byTraditional ChromatographyPurification ofTagged ProteinsScoutingProcess ProteomicsScale-UpWestern BlottingImmunoassaysSample Preparation Optimization Sample DetectionCovalent ProteinBinding• •• •••••••••••† In combination with appropriate chromatography resin.www.pall.com 5

PRODUCT SELECTION1.2 – Section 1.2Notes6