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4th Mission Report _KE3_Jan –Feb 2013Table of Contents1. ARMENIA (11th -17th February 2013) ............................................................................................................ 32. AZERBAIJAN (17th - 23rd March 2013) .......................................................................................................... 73. GEORGIA (29th January - 10th February 2013) ............................................................................................ 10Appendix 1 - List for Participants ........................................................................................................................... 20Regional Workshop for Laboratory Accreditation and Quality Control ................................................................. 20Hotel Courtyard Marriott, Tbilisi, Georgia. ,5 February, 2013 ............................................................................... 20Appendix 2 - Agenda of the Tbilisi Regional Training Workshop ........................................................................... 22Appendix 3 - Workplan for KE3 Mission 4 ............................................................................................................. 23Appendix 4 - Agenda for the Workshop for Quality Control and Gas Liquid Chromatography at Yerevan,Armenia ................................................................................................................................................................. 25Appendix 5 - List of Participants at Local Quality Control & GLC Training Workshop at EIMC in Armenia on14th February 2013 ............................................................................................................................................... 26Appendix 6 - Agenda for the Workshop for Laboratory Accreditation and Quality Control at NEA Tbilisi ........... 27Appendix 7 - List of Participants at Local Quality Control & GLC Training Workshops at NEMD in Baku,Azerbaijan on 20th-21st February ......................................................................................................................... 28Appendix 8 - Main issues and recommendations for Improving the Organic Micro-pollutant Analysis atEIMC, Yerevan, Armenia ........................................................................................................................................ 29Appendix 9 - Water Bodies at Risk in Pilot River Basins in Armenia...................................................................... 32Appendix 10 - Ecological categories for water quality of the rivers in Akhuryan ................................................. 33Appendix 11 - Draft Letter from First Deputy Minister of Environment ............................................................... 35Appendix 12 ........................................................................................................................................................... 36Page 2 of 36

4th Mission Report _KE3_Jan –Feb 2013IntroductionFollowing the success of the previous training missions in Eastern Europe, it was decided to continue withthe same approach for the Caucus Countries, viz.: to provide support and training for the laboratories ineach country. This encompassed Activity 1.5 “Support the analytical quality control assurance procedure”and Activity 1.6 “Assess the needs regarding laboratory infrastructure, equipment and training” bydeveloping the internal quality controls and the analysis of organo chlorine pesticides (OCPs) of each ofthe water quality laboratories. In addition the mission included the KE3’s input for the delineation ofwater bodies activity 2.2.The mission starting on 27th January and completed on 23rd February accordingto the operational plan is shown in Appendix 3.The tasks included visiting the three beneficiary countries to submit the following:1) Intensive training on the application of internal quality controls.2) Investigate the current methods and provide training for organochlorine pesticides (OCPs).3) Further discuss the water laboratories’ capabilities of sampling, analyzing benthic macroinvertebratesand their training needs.4) Assist each laboratory with their own analytical problems that could affect their analysis for theProject.5) Submit a formal regional training course on International Accreditation and Quality Control in Tbilisi.6) Submit extra local training workshops in each country tailored for their individual requirements.7) In addition to the laboratory inputs the KE3 worked with KE2, KE4 &KE5 on the delineation of waterbodies in the three countries for Activity 2.2.Also the KE3 attended a Team Meeting in Sofia, Bulgaria from 28th to 29th January 2013.1. ARMENIA (11TH -17TH FEBRUARY 2013)National Monitoring Laboratories’ TrainingEnvironmental Impact Monitoring Centre (EIMC), Ministry of Natural Protection, YerevanThe KE3 worked exclusively with the “Environmental Impact Monitoring Centre” (EIMC) under theMinistry of Nature Protection of the Republic of Armenia and the work was facilitated by the following:Seyran Minasyan, Deputy Director of Environmental Impact Monitoring Centre (EIMC), Ministry of NaturalProtection,Vahagn Tonoyan, Project Country Water Management Expert,Vladimir Ter- Ghazaryan, Translator.Chromatography DepartmentThe Deputy Director, Seyran Minasyan requested that the work should be focused on theChromatography Department, and included training the following analysts:Arpine Hovhannisyan,Ani Varagyan ,Nune Nersisyan,Levon Ghurkasyan,Ruzanna Andreasyan.The chromatographic equipment was comprehensive, consisting of four GLCs including a Varian with anECD, Agilent 5975 with MS, Ubet 500 with FID and a new Perkin Elmer Clarius 400 also with FID. However,Page 3 of 36

4th Mission Report _KE3_Jan –Feb 2013owing to the high turnover of staff, lack of calibration standards and specific training, the Department wasstruggling to obtain reliable results. On inspection the following issues were noted:1) Sampling BottlesThe sampling bottles were too small (160ml), ideally the bottles should be 2000ml amber glass withground glass tops or caps with Teflon inserts. These smaller bottles were used because procuringextraction solvents (pentane or hexane) was very difficult in Armenia owing to import problems.Therefore it was decided that by using smaller bottles then less solvent would be required. Whilst thisseems logical, it should be noted that the extraction is also an important concentrating technique. If thesample is too small, then insufficient concentrations of OCPs can be extracted and therefore impossibleto be detected by GLC analysis. It is very unlikely that such small samples would be sufficient to obtainthe required limit of detection (LOD). It was recommended that the sampling bottles were replaced bylarger 2000ml amber glass bottles with ground glass tops or caps with Teflon inserts.2) Calibration StandardsThere was a serious problem owing to the lack of proper calibration standards for all the organic analysis.The situation for each type of analysis was as follows:i) There were no calibration standards for the OCPs. The current analyst relied on previous calibrationsobtained in 2006 by their predecessors. Chromatographic peaks and recoveries can change from day today; therefore using calibration data that is 7 years old is unacceptable. To resolve this, fresh standardsshould be used to prepare new acceptable calibration standards. It was recommended that the Projectobtains these training materials so that the KE3 could complete this vital training.ii) There was one old standard mix (23 parameters) for VOC analysis of limited amount and was out ofdate.iii) There were only 3 contaminated old standards for the aromatic hydrocarbons analysis.The staff had been using these very poor standards, which were inadequate for quantification to obtaincredible results for the WFD. It was recommended that Project tries to at least obtain the OCP standardsto complete this urgent training.3) Instrumental software:Each of the four GLCs had dedicated operating software. The staff had not been adequately trained onthese, which limited the application of the software. However the important elements of the analysiscould be easily obtained from the software such as:i.) The retention times.ii.) The areas and heights of the integrated peaks.iii.) The operational conditions of the GLC.iv.) The identification of the peaks.These elements were sufficient to carry out qualitative and quantitative analysis by using simplecalibration graphs and by processing this data either manually or with Excel Spreadsheets.An introductory training workshop on GLC analysis was presented by the KE3 to ensure the staff had aclearer understanding on how the results could be calculated. It was recommended that the staff couldalso continue to improve their knowledge of the system by further investigating the software together. InPage 4 of 36

4th Mission Report _KE3_Jan –Feb 2013Inga BadasyanThe staff had previously been trained by other projects on the sampling procedures of Benthic Macroinvertebrates(BMI). They had previously taken hydrobiological samples and analysed the BMIs. They stillrequire more training on the identification of the species, the calculation of biotic indices and EQRs.Quality ControlThe Laboratory participates in a number of external quality control schemes e.g. National ReferenceLaboratory of Slovakia scheme and had been ranked highly in them, which is a good quality controlindicator.However, the number of parameters were limited and no internal analytical quality controls wereundertaken. Therefore following the training workshop, it was recommended that the Departmentcommits to establishing internal AQCs for at least for the main nutrient parameters. The KE3 couldprovide distance training and support prior to his next visit.Training WorkshopA customized special local training workshop was held on 14th February, which included the followingtopics:i. Production of Standard Operating Procedures (SOPs);ii. Laboratory Information Management Systems (LIMS);iii. Introduction to Analytical Quality Control, Precision and Accuracy;iv. Introduction to GLC for the analysis of OCPs;v. Presentation of main challenges of the Chromatography Department.The full agenda is shown in Appendix 4. There were 22 participants, which are listed in Appendix 7. Theworkshop was very interactive and Seyran Minasyan, expressed his thanks for such a useful and intenseworkshop. He expected that the KE3 would provide further such important assistance many more timesduring the project.Recommendations1) For the organic micro-pollutant analysis there were a number of issues that need to be resolvedincluding:i.) Sampling bottlesii.) Extraction Proceduresiii.) Calibration Standardsiv.) GLC Softwarev.) GLC Carry Over - Ghost Peaksvi.) Laboratory EnvironmentThese issues were discussed in detail in the local Training Workshop on 14th February withrecommendations for further work the Laboratory needs to undertake to improve the OCP, VOC andPetroleum Aromatic Analysis. The details of these are outlined in Appendix 8.2) It was also recommended that the Project obtains the OCP standards so that the training for thisanalysis can be completed.Page 6 of 36

4th Mission Report _KE3_Jan –Feb 20133) It was recommended that the Department commits to establishing internal AQCs for at least the mainnutrient parameters. The KE3 could provide distance training and support prior to his next visit.4) For Hydrobiological Analysis, it was recommended that further support will be required from theProject to engage specialist NKEs to assist in identifying the species of the BMIs, and calculating the EQRsof the Water bodies. A request was also made to arrange a visit to a European Centre of Excellence forBMIs.Water Body Delineation (15th-16th February)A number of meetings were held between KE3, CWME Vahagn Tonoyan, KE4 & KE5 and Aram Gevorgyan,GIS Expert, to discuss the delineation of water bodies in the pilot areas of the Akuryan & MetsamorRivers.According to the ToR for the project the typology must apply the simple system A for the Water Body(WB) delineation of the WFD, which recommends applying the following descriptors:1) Altitude typologyhigh: >800 mmid-altitude: 200 to 800 mlowland: 800m), therefore this was of limited application.2) GeologycalcareoussiliceousorganicMost of pilot area is siliceous, therefore this was of limited application.3) Size typology based on catchment areasmall: 10 to 100 km2medium: >100 to 1 000 km2large: >1 000 to 10 000 km2very large: >10 000 km2Using this characterization 62 water bodies were identified, comprising 50 natural river water bodies &12 artificial water bodies.The KE3 reviewed the available physical/chemical data and the Armenian River Basin Analysis Report andwas able to identify at least five water bodies at risk from anthropogenic activities. This is summarised in adraft table in Appendix 9, which will be further developed when the relevant data has been retrieved.Armenia has recently introduced a national classification system with 5 categories for defining the statesof their water bodies. This takes into account the normal natural background physical/ chemicalparameters characteristics of the Akuryan River and has been used to asses the river at differentmonitoring points (Appendix 10). Although this is different from the WFD classification system, this couldstill be a useful tool to undertake a preliminary classification as required in the baseline study.2. AZERBAIJAN (17TH - 23RD MARCH 2013)Baku Monitoring Centre of Environmental Pollution 17th to 21st FebruaryPage 7 of 36

4th Mission Report _KE3_Jan –Feb 2013The laboratory training was focused exclusively in Baku at the National Environmental MonitoringDepartment (NEMD) of the Centre for Environmental Pollution Monitoring of the Ministry of Ecology andNatural Resources of the Azerbaijan Republic.During this mission the following senior staff directed the work:1) Arastun Hasanov, Director National Department Environmental Monitoring Azerbaijan2) Aliyev Vasif, Head of Laboratory3) Gulkar Mammadova, Deputy Head of Laboratory.Organo-Chlorine Pesticide (OCP) AnalysisTo date, the NEMD Central Laboratory still had not commissioned its new Agilent GLC, though it had beenremoved from its packaging and sited in the Chromatography Laboratory. According to the AnarMammadov, Manager of Sem-Az, the local supplier, the apparatus could not be commissioned owing tothe following issues:i. A UPS was required, as the electrical supplies in Baku were unreliable.ii. An earth connection needed to be installed in the electrical sockets.iii. For the method to operate, solvents and calibration standards were required.iv. A dedicated computer is required to operate the GLC.The Agilent GLC was funded by another EU project focusing on analyzing transformer oils for PCBs.Therefore the NEMD were waiting for the extra funding for these other requirements. However after anumber of discussions with the senior staff and with Anar Mammadov, the KE3 was assured that theseissues would be all resolved in order to commission the equipment, and appropriate training would alsobe submitted. Sem-Az not only commission the analytical equipment but they also assist their clients indeveloping the analytical methods including the extraction procedures. If this service is provided well andin time then the OCP method should be ready for the Project Joint Field Survey (JFS). The KE3 supplied theNEMD and Sem-Az with hard copies of standard methods for the analysis of OCPs in water. He alsopresented an introductory local training course on important aspects of GLC analysis.As a possible contingency, the KE3 was introduced to another private laboratory AZEKOLAB, headed byBehruz Suleymanov. This laboratory had the state of the art equipment for all types of environmentalanalyses and also had undertaken excellent research into OCP analysis. It might be possible to use theiranalytical services as a last resort, but AZEKOLAB would have to obtain permission from their mainsponsor BP. However, it is more likely they could assist NEMD by advising on the optimum analyticalmethods.Analytical Quality ControlsThe Water Analysis Department carries out internal quality control analysis for certain parameters such asnitrates ammonia and phosphates. However they do not have a statistical system to check these resultsor apply Shewhart Charts. In addition the results were quoted to four decimal places, when the LOD wasonly two; therefore it was recommended that in this situation to quote only to two decimal places.The staff were all intensively trained by a training workshop and hands on training on how to developtheir AQC results statistically and to construct Shewhart charts.Hydrobiological AnalysisPage 8 of 36

4th Mission Report _KE3_Jan –Feb 2013The KE3 reviewed the latest developments regarding the BMI analysis with Ms Vafa Nasibova, Head ofBiomonitoring.The staff had previously been trained by other projects on the sampling of Benthic Macro-invertebrates(BMI). They have previously taken hydrobiological samples and analysed the BMIs. They still require moretraining on the identification of the species, the calculation of biotic indices and EQRs.Training CoursesAs the Department already does analyse quality controls, it requires only a few extra simple steps toconstruct the Shewhart chart and calculate the acceptable precision. With this in mind, the KE3 submitteda local AQC workshop training on the theoretical principles of internal quality controls, and was followedup by undertaking an exercise with the analysts using their own previous data to calculate the charts andprecision statistics.The local training workshop was presented on 20th February, to 22 trainees (Appendix 7) and covered thefollowing topics:i. Production of Standard Operating Procedures (SOPs)ii. Laboratory Information Management Systems (LIMS)iii. Introduction to Analytical Quality Control, Precision and Accuracyiv. The application of Excel Spreadsheets to calculate the AQC parameters.On 21st February the KE3 submitted further training, working with 7 analysts (Appendix 7) to apply simpleExcel Spreadsheets to calculate the statistical parameters using their own results for Ammonia tocalculate the precision of their method and to construct the Shewhart Chart. Following this hands onapproach, all the delegates were confident in undertaking these calculations on their own. The electroniccopy of the calculation was submitted to them as a reference example. Therefore there should be noproblem for the Department to produce their own correct internal quality controls for most of theirparameters in time for the JFS. Furthermore, the KE3 would be available to provide any further support tofacilitate this by distance training.On 21st February the KE3 also submitted further local training course on the GLC techniques used foranalysing OCP to 6 trainees (Appendix 7).Water Body Delineation 22nd – 23rd FebruaryAn initial meeting was held between KE3 and KE2, KE4, KE5, GIS Expert , Rafig Verdiyev CWM, FaridGarayev Translator.It was decided that to progress the delineation of the water bodies at risk Chapter 3 of the RBA wasrequired, which focuses on Pressures and Impacts but this had still to be produced. Therefore the KE3arranged a meeting with the author, Professor Fada Imarov, at Baku State University on 22nd February.Prof. Fada stated that the main pressures on the Project pilot rivers were:i. Manmade reservoirs on 3 of the 4 rivers which impacting on the normal flows of the rivers andcause dried rivers during the summer time. Uncontrolled water abstraction for irrigation.ii. Uncontrolled water abstraction for irrigation.iii. Illegal logging for fuel, causing deforestation, - this has diminished recently owing to gas beingsupplied to more communities.Page 9 of 36

4th Mission Report _KE3_Jan –Feb 2013iv. Over-grazing of cattle in the plains and mountain regions causing desertification and erosion andtherefore producing excess silt in water.v. Uncontrolled channels being made by farmers to divert the natural flows of small streams tocombine together to produce larger flows.vi. Mining for marble, where the washings are discharged into the rivers.vii. One of the largest iron mines in the Caucuses, where a proposal to construct an iron smelting andsteel works is being considered.Though this was useful information, Chapter 3 should identify the locations of these anthropogenicactivities so the water bodies at risk can be identified. Prof. Fada agreed to confer with Rafig Verdiyev,Hydrology Lecture, Baku State University and Matanat Avazova, Deputy Director, Monitoring Centre ofEnvironmental Pollution to ensure this information would be in the report.A review meeting was held with KE2, KE4, KE5, the GIS Expert, Rafig Verdiyev, and Matanat Avazova on23rd March. Some 50 water bodies had been identified. This information needed to be produced in mapform and Rafig and Matanat agreed to assist Prof. Fada to define the exact locations of the water bodiesat risk, together with the water quality data.3. GEORGIA (29TH JANUARY - 10TH FEBRUARY 2013)The Department of Environmental Pollution Monitoring of the National Environmental Agency (NEA) ofthe Ministry of Environment Protection of Georgia is responsible for the ambient surface water qualitymonitoring Georgia, and has a central laboratory in Tbilisi. It also is responsible for the National Black SeaMonitoring Laboratory in Batumi. The KE3 worked with staff in both laboratories and submitted aRegional AQC Workshop at the Conference Centre in Marriot Hotel in Tbilisi.Laboratory TrainingCentral Laboratory of the Department of Environment Pollution Monitoring (NEA). Tbilisi 30th January to5th February 2013A number of meetings were held with management staff to plan the work schedule and to update on theprogress of the training.PresentMarina Arabidze NEA Georgia Head of Environment Pollution Monitoring DepartmentElina Bakradze , Head of Atmospheric Air, Water and Soil Analyses LaboratoryAnna Natriashvili, Senior Microbiologist.Zurab Jincharadze, Project Deputy Team Leader1) Organo Chlorine PesticidesThe Chromatography Department consists of two staff:Maka Khvedeliani, Main Chemist, Chromatographer Water SpecialistNino Vasadze, Main Chemist, Chromatographer Soil SpecialistThe Department currently analyzed five OCPs using their GLC with a liquid/liquid extraction techniqueusing Hexane/acetone. There were a number of issues noted:Page 10 of 36

4th Mission Report _KE3_Jan –Feb 2013i) The GLC column is DN- 5, 25mX 0.32mmX0.24µ manufactured by DANI (Italian Co.), which isrecommended for EPA method 608/808, however the ISO 6468 method was being used instead. To assistin identifying the peaks it was recommended to obtain this EPA method.ii) The Department had obtained a new calibration standard of 17 components but this had not been usedas the staff are waiting for a special “certan” vial to be delivered in order to store this standard correctly.iii) Initially, the results from the chromatograms and were not clear, but following further investigations ofthe software, the results were clarified. It was recommended that a SOP is produced to clarify theprocedures.iv) The extraction efficiency had been determined for each of the five pesticides, however this couldchange depending on the water sample characteristics, therefore it was recommended that an internalcalibration standard is used.v) The current sample bottles were HDPP plastic bottles, however, according to the method, these werenot acceptable. It was recommended that these be replaced by 2l amber glass bottles with ground glassstoppers or caps with Teflon inserts.vi) The GLC was not being used at the time, as the high purity nitrogen had been depleted and theDepartment was waiting for new cylinders to be delivered. The Department tried to use another source ofnitrogen, but this was not pure enough. It was recommended to keep a reserve cylinder in stock, and alsoinstall carbon filters inline from the gas cylinder to the GLC to prevent gross contamination.The current staff were relatively new on GLC analyses (6 months) and the training and interfacing of theequipment was undertaken by Vakhtan g Ugrekhelidze, GLC expert (Georgian Isotopic Institute). It wasrecommended that he should be further engaged to assist in the above recommendations.2) Analytical Quality ControlThe Laboratory participates in a number of external quality control schemes, e.g. Accreditation Instituteof Slovakia (SNAS) scheme and had been ranked highly in them which is a good quality control indicator.In addition the laboratory analyses 7 parameters (F, CL, NO2, Br, NO3, PO4, SO4), which includeinternal AQCs to produce Shewhart charts, using independent software. However, internal AQCs were stillrequired for about thirty other parameters. Elina Bakradze was previously responsible for quality controlbut had recently been promoted and this work was transferred to Ms. Gulchina Kuchava, who wascurrently learning these techniques. Elina has written all the procedures Quality Manual, SOP, SP, GP, etc.which are necessary for the ISO 17025 and has provided the training for Gulchina Kuchava on qualitymanual and now the laboratory are progressing the implementation of those procedures. Therefore thetraining submitted during this mission should have proved very useful as a support for this.Recently the certified experts from the “ICI project –Capacity Building on the Water Monitoring andManagement in Georgia, 2011-2013 (Georgia Waters) visited the NEA laboratory and have made anauditi on 3 components viz. , Ion Chromatography analyses and Microbiology. They maderecommendations in March 2013, which have now been implemented. They have concluded that all isnow perfect apart from an amendment on purchasing new reagents.)The KE3 presented a local training workshop on quality control 4th February for 7 staff, and the rest of thepertinent staff attended the more comprehensive Regional Training Seminar on 5th February. In addition,the KE3 submitted a separate training on the UK method validation software to Elina Bakradze.Unfortunately the software was unable to function correctly on the laboratory’s computers, though it hadPage 11 of 36

4th Mission Report _KE3_Jan –Feb 2013worked well in other countries. This appeared to be caused by a Windows problem and advice wassubmitted by a computer colleague to try to overcome this, however this is still being investigated.It was recommended that as all the relevant staff had been trained during the mission and the Laboratoryalready had internal AQC software, it should be relatively easy to establish AQC’s for most of the majorparameters. These should be established soon prior to the joint field survey, the KE3 would be availableto provide any further support to facilitate this by distance training.Hydrobiological AnalysisPresent Irakli Kordzaia (NEA Senior Hydrobiologist)Irakli had previously received extensive training on sampling for BMIs by the Finnish HydrobiologicalInstitute (SYKE) and had been audited on his skills, which were considered to be excellent.He has a working knowledge of AQUEM and ASTERIX Software and was able to use this to determinebiological index by the relatively simple BMWP system, which does not require the BMI species results.However to calculate the EQR one needs to include other advanced biotic indices that do require theidentification of the species. He requested further training and assistance for determining the specieslevel, which should include assistance from BMI experts and also a study/training tour at a EuropeanCentre of ExcellenceTraining WorkshopsThe formal regional workshop was held on 5th February. This covered the following topicsi. Introduction to Laboratory Accreditation 17025.ii. Production of Standard Operating Procedures (SOPs).iii. Laboratory Information Management Systems (LIMS).iv. Introduction to Analytical Quality Control, Precision and Accuracy.v. Practical Demonstration of Internal AQC system used in Hydromet, Moldovavi. Validation of Analytical Methods.vii. Practical Demonstration of Method Validation Software used by Hydromet Moldovaviii. An introduction to the processing techniques and quality assurance used for Hydrobiologicalanalysis at the Academy of Sciences in UkraineIx An introduction to hydrobiological software (ASTERIX and AQUA BIOBASE), which are used at theAcademy of Sciences in Ukraine for improving quality assuranceThe full agenda is shown in Appendix 2. There were 19 delegates that attended (Appendix 1) and was veryinteractive indicating that the main points were understood well.Marina Arabidze, Head of Environment Pollution Department, requested that additional training shouldbe submitted to the staff who were unable to attend. Therefore a local training workshop on internalquality control was also submitted by KE3 to the following seven analysts.Maka Khvedeliani, Main ChemistNino Vasadze, Main ChemistLali Salamashvili, Main ChemistMarina Chigitashvili, Main ChemistKetevan Kvatashidze, Senior ChemistAnna Natriashvili, Senior MicrobiologistEka Qitoshvili, Senior Chemist.Page 12 of 36

4th Mission Report _KE3_Jan –Feb 2013The details of the course is shown in Appendix 6.RecommendationsFor the GLC analysis the following recommendations should be considered:i.) Obtain the EPA method 608/808 to assist in identifying the current chromatographic peaks.ii.) Produce a SOP to clarify the operational, calibration & quantification procedures.iii.) Research into the inclusion of an internal calibration standard within the samples.iv.) Replaced the current HDPP plastic bottles with 2l amber glass bottles with ground glass stoppers orcaps with Teflon inserts.v.) Keep a reserve nitrogen carrier gas cylinder in stock.vi.) Install carbon filters inline from the gas cylinder to the GLC to prevent gross contamination.vii.) Engage Vakhtang Ugrekhelidre, GLC expert (Georgian Isotopic Institute), to assist in the aboverecommendations.viii.) The project should obtain a calibration OCP standard to complete the training.For the quality control the water department should increase their number of parameters that apply AQCstandards to check their analysis ready for the JFS.For the hydrobiological analysis, further training and assistance for determining the BMI species level andEQR values, is required by the project plus for the project to a study/training tour at a European Centre ofExcellence. To complete the training for the calculation of the BMI biotic indices the Aquabiobasesoftware will be required by the Project.Batumi Laboratory – the Black Sea Monitoring Division, Environment Pollution Monitoring DepartmentNEA- 6th -9th FebruaryFollowing discussions with following management staff:Marina Arabidze, NEA Georgia Head of Environment Pollution Monitoring DepartmentIrine Baramidze, NEA , Head of Water Quality Laboratory,it was decided that two important analytical apparatus for the analysis of samples for the Project requiredurgent attention: The Perkin Elmer Atomic Absorption Spectrophotometer (AAS) 600 The Perkin Fluorescence Spectrophotometer (FS)451) Atomic Absorption SpectrophotometerThe AAS model (600) is advanced equipment, using a carbon furnace with the Zeeman facility, whichshould be able to analyse most of the heavy metals very accurately and to a very low LOD. Unfortunatelythe Perkin Elmer Agent in Georgia apparently had not been very helpful and had quoted very high pricesjust to diagnose the problems, let alone repair the instrument. Also the agent appeared to have maderecommendations, which were expensive and counterproductive, e.g. it was recommended that the clientneeded a UPS system. However, it really was not feasible to use such a system, as the instrument usesvery high currents of 32 amps and most UPS systems cannot normally operate at such high currents.The following issues were addressed:Page 13 of 36

4th Mission Report _KE3_Jan –Feb 2013i) To accommodate this erroneous recommendation of installing a UPS, an inadequate 13 amp householdplug was used for the high power electrical supply. Furthermore other inadequate connections usingsimple wire connections with insulating tape had been used rather than the proper connectors.This electrical system was considered dangerous by the KE3 and he recommended that a professionalelectrician reconfigure the connections , who was immediately engaged to undertake this. He installed, asa temporary measure, a heavy duty 2 pole switch, with the third earth wire directly connected. Also thedangerous connection was replaced with a direct heavy duty connector. This was satisfactory as atemporary basis but it was recommended that the 2 pole switch should be replaced with a 3 pole switchand a heavy duty plug and socket should be installed.ii) Following the rectification of the electrical supply, the operation of the AAS 600 was investigated. Therewere three hollow cathode lamps (HCL) installed for Zn, Cu, Fe Ni and Pb analysis and also an EDL lamp forCadmium. The AAS was computer controlled using Perkin Elmer Windows software. According to thecomputer display, the AAS appeared to initialise correctly for the Spectrophotometer, Furnace andAutosampler. However the highest output energies from the lamps was only about 30% which seemedtoo low and the EDL lamp produced no energy at all. When the analysis was carried out even for the topstandards of copper of 5µl of 50µg/l, no atomic absorption was noted with or without backgroundcorrection.iii) The carbon furnace tube was removed and the energy was checked again and no difference wasnoted, indicating that the alignment was satisfactory.iv) The autosampler appeared to function correctly and to verify that this was not causing the problemthe highest copper standard (50µg/l) was manually injected and again no atomic absorption was noted.v) The above checks indicated that this problem could originate from a faulty detector as it seemedunlikely that all the lamps were faulty. It was recommended that the above information is passed on tothe Perkin Elmer Engineer, to assist the diagnosis and resolution of the problem.2) Fluorescence SpectrophotometerThe Perkin Fluorescence Spectrophotometer (FS 45) had been delivered about 5 years ago but had neverreally been used. It can be used for the analysis of any compound that fluoresce or luminesce such as:petroleum hydrocarbon products, and polyaromatic hydrocarbons. This technique is useful, as theindividual methods are usually very specific and avoid interferences by other compounds. Furthermore itis also sensitive, achieving low limits of detection, especially if the excitation and emission wavelengthsare specified within the method. The FS 45 had limited facilities compared to other models, so that the slitwidths were fixed at 10 nm and could not be changed, and there were no other accessories such as anautosampler and flow cells. It had just one cell holder and two 1 cm silica cells were supplied. Thissimplified the operation and settings for the instrument.The following issues were addressed:i) The FS45 was computer controlled but the CD drive was faulty, and was replaced and the WinFlowsoftware was reinstalled.ii) There were a number of methods already installed in the software, which were all tested by KE3 butthe one that was easily applicable was the “Time Drive” method. With this method one could specify theemission and excitation wavelengths and then scan the fluorescence over a period of time, which couldbe specified by the operator.Page 14 of 36

4th Mission Report _KE3_Jan –Feb 2013When the Time Drive method is set up, one can preset the emission, and excitation wavelengths togetherwith the duration of each integration period (set at 5 seconds). The duration of the scan could also be sete.g. 100 seconds. One could also set up the file name (td file) so that the graphical results could be saved.The instrument uses a simple traffic light icon to be clicked to start the analysis, which then initiated the100 second scan showing the fluorescence emission value graphically.iii) Using the above technique, this method was tested by using a range of crude oil standardsconcentrations from 0.5 to 10µg/l in hexane, which produced emissions from 200 to about 996, with ablank of about 27. This indicated that the LOD for this method could, in theory, reach at least 0.05µg/l,though would depend on the liquid/liquid extraction, and concentration efficiencies.iv) The KE3 recommended that this method should be developed for specific hydro carbons such as crudeoil, diesel oils & total petroleum products where standard methods are available by:a. Initially making up at least 6 concentration standards in the extraction solvent (hexane) to producea calibration graph to determine the linearity range.b. Following this, carry out the ISO extraction procedure on a similar series of standards made up indistilled water in order to determine the ideal recoveries.c. Repeat (b) using standards in the Pilot River water of the Chorokhi – Adjaristkali taken from a goodreference site (site with no anthropogenic activity so that the water should be pure as possible) toestablish the extraction efficiencies from the river water.d. It was also recommended to repeat this process from similar pure water from the Black Sea, whichwould be important for the department in analyzing the sea and also for the imminent new EUsister project investigating the coastal regions.If the Department does not have the available time to undertake this method development, it could be anideal project for a research M.Sc. postgraduate study, which would also minimise costs.v) The FS 45 does have other useful facilities, such as scanning fluorescence at differing wavelengths,which could be useful for determining new methods for compounds where there are no ISO methodsavailable. When this is required, the KE3 expert could investigate this new operational procedure duringfuture missions to further develop the application of this equipment.3) Other Equipment ProblemsThe KE3 also checked the inoperative microwave digester, unfortunately there was insufficient time tofully investigate this problem and it was recommended that this is progressed in the next appropriatemission as this apparatus is not as vital as the others.The Laboratory also had a Scalar Continuous Bubble Flow Analyser dedicated for measuring nitrates,phosphates and ammonia. This had recently broken down and appeared to have a power problem andwas due to be fixed soon.The Chemical Laboratory had so many problems with their expensive sensitive equipment, could becaused by poor electrical supplies. It was recommended that, where possible, to using anti surge electricalsockets, and power regulators to avoid electrical spikes, and safeguard the equipment, though this mightnot be possible for the AAS.4) Analytical Quality ControlPage 15 of 36

4th Mission Report _KE3_Jan –Feb 2013No internal quality controls were applied at the laboratory; therefore the KE3 was requested to submit asimple introductory training course to the staff who did not attend the regional course. It wasrecommended that the laboratory establish AQC standards at least for the nutrient parameters prior tothe JFS.Hydrobiological AnalysisA meeting was held on 8th February between the KE3 and all the main hydrobiologists to determine theirpossible input in the Project.Present1. Mr George Komakhidze, Head of the Black Sea Monitoring Division2. Ms Marina Mgeladze, Chief Monitoring Specialist,3. Mr Revaz Diasamidze, Hydro-biologist, benthos specialist4. Mr Revaz Goradze, Hydro-Fauna Specialist5. Ms Tsiuri Gvarishvili, Specialist of phytoplankton6. Ms Dodo Zghenti, Water Microbiologist7. Ms Eteri Mikashavidze, Specialist of macro zoobenthos8. Ms Meri Khalvashi, Specialist of zooplankton9. Mr Archil Guchmanidze, Specialist of marine mammals10 Ms Marina Arabidze, Head of Environment Pollution Monitoring DepartmentThe following recommendations were made:i) During this year the two experts of BMI at the Centre could assist with the Georgian joint field survey.They had previously assisted in similar surveys in the Kura Projects, so their experience should prove veryuseful.ii) They would work together with Irakli Kordzaia, BMI expert at NEA Tbilisi.iii) It was suggested that the samples could be taken in duplicate, with one set of samples analysed atTbilisi, and the other set at the Batumi Centre. The Project BMI Expert could assist both laboratories toidentify the BMIs, train on biotic indices, EQRs and compare their results. Therefore there would becapacity building at both laboratories and would initiate closer co-operation between them.iv) It was also suggested that students in BMI analysis at the local universities should also participate withthe JFS, to ensure sustainability for this type of monitoring.v) The following year other hydrobiological elements such as phytoplankton could be included and wouldtherefore involve more staff from the Batumi Centre.RecommendationsThe following recommendations should be considered:For the AAS 600:i.) The electrical supply should be changed such that the 2 pole switch should be replaced with a 3 poleswitch and a heavy duty plug and socket should be installed.ii.) The section of the report outlining the work undertaken in this mission to diagnose the lamp energyproblem should be passed on to Perkin Elmer to reduce their time in resolving this problem.Page 16 of 36

4th Mission Report _KE3_Jan –Feb 2013For the FS 45:i.) To ensure that the operational method is clear to all analysts, an SOP should be produced for theTime drive method.ii.) The Time drive method should be developed for specific hydro carbons such as crude oil, diesel oils& total petroleum products.The problems with the AAS in Batumi and Tbilisi are serious, as it means that the MoE cannot analyseheavy metals accurately in water. These instruments require immediate attention, but unfortunately theDepartment’s resources are limited. Therefore the Deputy Minister has submitted an official letter to theproject to assist in resolving this problem to repair the AASs. This letter is in Appendix 11 and should bediscussed by the Project Senior Management to progress this urgent issue.For the Hydrobiological Analysis:During this year the two experts of BMI at the Centre could assist with the Georgian joint field survey.Samples could be taken in duplicate with one set of samples analysed at Tbilisi and the other set at theBatumi Centre.For Quality Control:Establish AQC standards at least for the nutrient parameters prior to the JFS.WB DelineationThe KE3 was unable to meet with other Key Experts to work together for the delineation of the waterbodies. However they did meet up the GIS experts and the other stakeholders and they will liaise with KE3to ensure the data for the water bodies at risk will be included.Summary & ConclusionsActivity 1.5The KE3 visited the three Caucus Countries providing intense training to the water analysis departmentsto ensure that they could establish and/or develop the own internal AQC systems. This training included aregional training workshop in Tbilisi and then supplemented by local customised workshops in eachcountry. Where appropriate this was also further supported by hands-on training of the staff using theirown analytical results.It is essential that most of these systems are established prior to the Joint Field Survey in June 2013.Most countries should have had sufficient training to undertake this challenge, however if necessary theKE3 could provide distance training and support prior to his next visit.Georgia has already established this system, but only for about five parameters and needs to develop thisfor more parameters. Azerbaijan already includes internal AQC’ s for a few parameters, but needs todevelop this to calculate the precision statistics and the Shewhart charts. Armenia has still to incorporatesuch a system, at least for the nutrient parameters.Outputs1) One regional training workshop with five PowerPoint presentations was submitted to 19 delegatesElectronic copies UK method validation software (AQC99)was also submitted together with a demoversion of the Hydrobiological software Aquabiobase.Page 17 of 36

4th Mission Report _KE3_Jan –Feb 20132) At least one extra local training course was submitted to each country, together with the associatedtraining materials.3) Training Certificates were submitted to the 19 delegates at the regional workshop. Templates havebeen also produced by KE3 for the other local workshops -an example is shown in Appendix 12.These certificates should be presented to all the participants, as a record of their training for inclusion ineach country’s ISO 17025 quality manual.Activity 1.6Georgia and Armenia both analyse for OCPs and Azerbaijan has the equipment. Both Georgia andArmenia need to make changes with their sampling bottles, extraction and analysis procedures.Azerbaijan needs to commission the GLC and develop the method, which the supplier can assist with.The KE3 worked with the analysts to provide further training and making recommendations to developand improve the methods. One key material prevented the KE3 from completing his training and this wasthe OCP mixed standard, it is essential for the Project to obtain this, so that the countries can completethis analysis to produce credible results following the JFS.The Batumi Coastal Laboratory in Georgia had some serious problems with their Atomic AbsorptionSpectrophotometer and Fluorescence Spectrophotometer, which are important to analyse WFDparameters. The KE3 spent three days working with the senior staff to resolve these problems.The Fluorescence Spectrophotometer’s problems were resolved so that it was operational. The AtomicAbsorption Spectrophotometer problem was extensively diagnosed has been confirmed to be a lampenergy detection problem.The Hydrobiological equipment has been installed in each laboratory and all countries have been trainedin the sampling of BMIs. However, they have requested further training on the identification of the BMIsand the procedures for processing the results. This should be undertaken by a non-key specialist.Furthermore as a tool to process the results and to complete the training the countries require aspecialised software called Aquabiobase, which was demonstrated at the regional workshop.Outputs1) The KE3 has provided training, support and recommendations to improve the OCP analysis in eachcountry. The details can be referred to by the stakeholders in this mission report.2) At Batumi NEA the KE3 managed to make the Fluorescence Spectrophotometer operational after it notbeing used since new for over 5 years. The staff were submitted hands-on training and were confident inusing the equipment to analyse petroleum oils. Recommendations were made tofurther develop the application of this apparatus.3) Within this report is a list of the issues addressed the problem of the AAS, which could be submitted tothe manufacturer Perkin Elmer to resolve the problem quickly.4) The KE3 has been working with the Project Managers to produce the ToR for the non-key expertsrequired for the BMI analysis.Activity 2.2The KE3 worked with the other Key Experts in Armenia and Azerbaijan in developing the delineation ofthe water bodies at risk. To date only Armenia had submitted information to start this process (Appenix 9Page 18 of 36

4th Mission Report _KE3_Jan –Feb 2013&10) and more data and information is still being obtained by the stakeholders. Azerbaijan has still tosubmit the RBA for Chapter 3, so that this work can be completedOutputsThe KE3 assisted the KE4 to produce the draft water body delineation report.Finally it is proposed that a separate recommendation report should be produced by KE3 to address theimportant issue of the training materials that the Project require to complete the training on the OCPanalysis and for the BMI processing, subject to the approval of the senior management and stakeholders.Page 19 of 36

Environmental Protection ofInternational River Basins ProjectContract No. 2011/279-666This project is funded byThe European UnionA project implemented by a Consortiumled by Hulla & Co. Human Dynamics KGAppendix 1 - List for ParticipantsRegional Workshop for Laboratory Accreditation and Quality ControlHotel Courtyard Marriott, Tbilisi, Georgia, 5 February 2013# Name Organization Position Country/CityHuman Dynamics/EPIRB Project Representatives1 Mr Zurab Jincharadze HD/EPIRBKE 2 - Deputy TeamLeader/River BasinGeorgia/TbilisiManagement2 Mr Ralph Michael Jackman HD/EPIRBKE 3 - Biology/EcologyMonitoringMalaysia/Penang3 Mr Vahagn Tonoyan HD/EPIRB CWME Armenia Armenia/Yerevan4 Mr Rustam Rajabov HD/EPIRB CWME Azerbaijan Azerbaijan/BakuInvited International Guest Speaker5 Mr Oleksandr UsovInstitute of Hydrobiology National Academy of Science ofUkraineProfessorUkraine/KievRepresentatives of Beneficiary Institutions6 Mr Vardan KaryanEnvironmental Impact Monitoring Centre of the Ministry of Chief Specialist,Nature ProtectionBiomonitoringArmenia/Yerevan7 Mr Seyran MinasyanEnvironmental Impact Monitoring Centre of the Ministry ofNature ProtectionDeputy DirectorArmenia/Yerevan8 Mr Davit DavtyanEnvironmental Impact Monitoring Centre of the Ministry ofNature ProtectionChief SpecialistArmenia/Yerevan9 Ms Lusine GevorgyanEnvironmental Impact Monitoring Centre of the Ministry ofNature ProtectionChief SpecialistArmenia/Yerevan10 Mr Arastun Hasanov Environmental Pollution Monitoring Department, Head of Department Azerbaijan/Baku11 Mr Vasif Aliyev Environmental Pollution Monitoring Centre Head of Centre Azerbaijan/BakuPage 20 of 36

4th Mission Report _KE3_Jan –Feb 201312 Ms Gulkhar Mammadova Environmental Pollution Monitoring Centre Deputy Head of Centre Azerbaijan/Baku13 Ms Vafa NasibovaSpecialist of 1 Category ofEnvironmental Pollution Monitoring Centrebiomonitoring LabAzerbaijan/Baku14 Ms Irina BaramidzeThe Black Sea Environmental Laboratory, NationalEnvironmental AgencyChief SpecialistGeorgia/Batumi15 Ms. Marina MgeladzeThe Black Sea Environmental Laboratory, NationalEnvironmental AgencyGeorgia/Batumi16 Ms Marina ArabidzeEnvironmental Pollution Monitoring Department, NationalEnv. Agency, MEPHead of DepartmentGeorgia/Tbilisi17 Mr Irakli Kordzaia National Environmental Agency, MEP Hydrobiologist Georgia/Tbilisi18 Ms. Elena Bakradze National Environmental Agency, MEP Head of Laboratory Georgia/Tbilisi19 Ms. Gulchina Kuchava National Environmental Agency, MEP QA/QC Officer Georgia/TbilisiPage 21 of 36

4th Mission Report _KE3_Jan –Feb 2013Appendix 2 - Agenda of the Tbilisi Regional Training WorkshopIntroduction to the Procedures and Techniques Required for International Accreditation And Quality Control for Water LaboratoriesTechnical Assistance & Capacity BuildingCourse Laboratory Accreditation and Quality ControlDate5 th February 2013 (1 day)Objectives Introduction to the procedures and techniques required for international accreditation for water laboratoriesTarget group Staff involved in River Basin Management MonitoringMaterials Computer PowerPoint Presentations with Projector, Flip Charts, supporting information and softwareLocation Hotel Courtyard by MarriotDAY 1Time Method Content Lecturer9.30 – 9.45 Welcome to delegates Senior Manager of NEA (TBA)9.45 – 10.15 Introduction to the Project and the Training Workshop Zurab Jincharadze, Project Deputy Team Leader10.15 –11.00 Lecture 1 Introduction to Laboratory Accreditation 17025 Michael Jackman, Project Biology & Ecology Expert11.00 – 11.15 Coffee Break11.15 – 12.15 Lecture 2 Production of Standard Operating Procedures (SOPs) & Michael Jackman, Project Biology & Ecology ExpertLaboratory Information Management Systems (LIMS)Introduction to Analytical Quality Control, Precision andAccuracy12.15 – 13.00 Questions and Discussions Zurab Jincharadze, DTL13.00 – 14.00 Lunch14.00 – 15.00 Lecture 3 Validation of Analytical Methods & Associated Software Michael Jackman, Project Biology & Ecology Expert15.30 – 16.00 Lecture 4 Guidance on the classification Benthic Macro Invertebrate by Prof. Usov Oleksandr Guest Speaker from UA,applying customized softwareHydrobiologist16.00 – 16.15 Coffee Break16.15 – 16.45 Lecture 5 Sampling Benthic Macro Invertebrates & associated software Prof. Usov Oleksandr -Guest Speaker from UA,Hydrobiologist16.45 – 17.30 Questions and DiscussionsZurab Jincharadze, DTLPresentation of Certificates19:00 DinnerPage 22 of 36

Environmental Protection ofInternational River Basins ProjectContract No. 2011/279-666This project is funded byThe European UnionA project implemented by a Consortiumled by Hulla & Co. Human Dynamics KGAppendix 3 - Workplan for KE3 Mission 4AGENDATravel day 30-Jan-13 KE4 arrives to Batumi at 17:00 via Istanbul by plane; NEA/GE beneficiary - at 14:00 by train from Tbilisi; overnight stay in BatumiGeorgia 31-Jan-13 Batumi: KE3 and NEA/GE beneficiary work at the Black Sea Monitoring Division's Lab on the Act ivity1.5/1.6 tasksGeorgia 1-Feb-13 Batumi: KE3 and NEA/GE beneficiary work at the Black Sea Monitoring Division's Lab on the Act ivity1.5/1.6 tasksTravel day 2-Feb-13 Batumi/Travel: KE3 and NEA/GE beneficiary travel to Tbilisi by trainGeorgia 3-Feb-13 Tbilisi: KE3 works on QA/QC mission reportGeorgia 4-Feb-13 Tbilisi: KE3 works on Act ivity1.5/1.6 tasks at NEA Pollution Monitoring Department LabGeorgia 5-Feb-13Tbilisi: cameral work on Water Body identification and delineation with the beneficiaries: NEA Dept. of Monitoring, Hydromel, Eng.GeologyGeorgia 6-Feb-13Tbilisi: full day Regional Workshop (GE, AM, AZ benef., CWMEs, DTL, KE3) in Courtyard Marriott on Laboratory Accreditation andQuality ControlTravel day 7-Feb-13GE workshop participants travel to Batumi by train; AZ participants - to Baku by plain; AM participants and KE3 - to Yerevan byminivanArmenia 8-Feb-13 Yerevan: KE3 works on Act ivity1.5/1.6 tasks at MNP/EIMC LaboratoryArmenia 9-Feb-13 Yerevan: KE3 works on Act ivity1.5/1.6 tasks at MNP/EIMC LaboratoryTravel day 10-Feb-13 KE4 arrives to Yerevan at 20:20; KE5 - at 15:25; overnight stay in Yerevan; KE3 works on QA/QC mission reportArmenia 11-Feb-13Yerevan: cameral work on Water Body identification and delineation with the beneficiaries: WRMA, Hydromet, EIMC, Hydro-Geology CentreArmenia 12-Feb-13Yerevan: cameral work on Water Body identification and delineation with the beneficiaries: WRMA, Hydromet, EIMC, Hydro-Geology CentreArmenia 13-Feb-13Yerevan/Travel: Water Body identification and delineation with the beneficiaries; the team travel to Tbilisi by car at 16:00;overnight in TbilisiGeorgia 14-Feb-13Tbilisi: cameral work on Water Body identification and delineation with the beneficiaries: NEA Dept. of Monitoring, Hydromel, Eng.GeologyGeorgia 15-Feb-13 Tbilisi: cameral work on Water Body identification and delineation with the beneficiaries: NEA Dept. of Monitoring, Hydromel, Eng.Page 23 of 36

4th Mission Report _KE3_Jan –Feb 2013GeologyGeorgia 16-Feb-13Tbilisi: cameral work on Water Body identification and delineation with the beneficiaries: NEA Dept. of Monitoring, Hydromel, Eng.GeologyTravel day 17-Feb-13 The team (DTL, KE3, KE4, KE5) travel from to Baku by plane at 20:15; overnight stay in BakuAzerbaijan 18-Feb-13Baku: cameral work on Water Body identification and delineation with the beneficiaries: Monitoring Dept., Hydromel, Hydro-Geology ExpeditionAzerbaijan 19-Feb-13Baku: cameral work on Water Body identification and delineation with the beneficiaries: Monitoring Dept., Hydromel, Hydro-Geology ExpeditionAzerbaijan 20-Feb-13Baku: cameral work on Water Body identification and delineation with the beneficiaries: Monitoring Dept., Hydromel, Hydro-Geology ExpeditionAzerbaijan 21-Feb-13Baku/Travel: DTL leaves Baku at 12:30; KE4 - at 09:35; KE5 - at 16:10; KE3 works on Act 1.5/1.6) with the Env. Lab of the MoEMonitoring DepartmentAzerbaijan 22-Feb-13 Baku: KE3 works on Act 1.5/1.6) with the Env. Lab of the MoE Monitoring DepartmentTravel day 23-Feb-13 Baku/Travel: KE3 works on QA/QC mission report; KE3 leaves Baku at 20:30 (Baku-Doha-Kuala Lumpur-Penang)Page 24 of 36

Appendix 4 - Agenda for the Workshop for Quality Control and Gas Liquid Chromatography at Yerevan, ArmeniaTechnical Assistance & Capacity BuildingCourse Laboratory Quality Control & Introduction to Gas Liquid Chromatography for OCP AnalysisDate14 th February 2013 (1 day)Objectives Introduction to Internal AQC and GLC methods for OCP analysisTarget group Staff involved in River Basin Management MonitoringMaterials Computer PowerPoint Presentations with Projector, Flip Charts, supporting information and softwareLocation EIMS Central Laboratory, Yerevan, ArmeniaDAY 1Time Method Content Lecturer10.00 – 10.15 Introduction to Course Seyran Minusyan, Deputy Director10.15 – 10.45 Lecture 1 Production of Standard Operating Procedures (SOPs) & Michael Jackman, Project Biology & Ecology ExpertLaboratory Information Management Systems (LIMS)Introduction to Analytical Quality Control, Precision and Accuracy10.45 –11.15 Lecture 2 Introduction to Analytical Quality Control, Precision and Accuracy Michael Jackman, Project Biology & Ecology Expert11.15 – 11.30 Coffee Break11.30 – 12.15 Lecture 3 Introduction to GLC for the analysis of OCPs Michael Jackman Project Biology & Ecology Expert12.15 – 12.45 Lecture 4 Main issues concerning the Chromatography Department Michael Jackman Project Biology & Ecology Expert12.45 – 13.00 Questions and Discussions Seyran Minusyan,Deputy DirectorKEs Mission ReportsHuman Dynamics KG ConsortiumPage 25 of 36

Appendix 5 - List of Participants at Local Quality Control & GLC Training Workshop at EIMC in Armenia on 14th February 20131) Manana Zalieva2) Anna Aloyan3) Arpine Hovhannisyan4) Anna Zatikyan5) Lilit Mnoyan6) Melanya Arakeleyan7) Siranush Avagyan8) Hripsime Mkrtchyan9) Armine Gabrielyan10) Arpine Gabrielyan11) Lusine Gevorgyan12) Alina Zurnachyan13) Ani Varagyan14) Ruzan Manukyan15) Vardan Qaryan16) David Davdyan17) Nune Nersisyan18) Levon Ghurkasyan19) Shushanik Karapetyan20) Zanazan Nersisyan21) Hayk Hoveyan22) Ruzanna Andreasyan23) Seyan Minusyan,Page 26 of 36KEs Mission ReportsHuman Dynamics KG Consortium

Appendix 6 - Agenda for the Workshop for Laboratory Accreditation and Quality Control at NEA TbilisiTechnical Assistance & Capacity BuildingCourse Laboratory Accreditation and Quality ControlDate 4 th February 2013Objectives Introduction to the procedures and techniques required for international accreditation for water laboratoriesTarget group Staff involved in River Basin Management MonitoringMaterials Computer PowerPoint Presentations with Projector, Flip Charts, supporting information and softwareLocation NEA Pollution Control Head Office, Tbilisi, GeorgiaDAY 1Time Method Content Lecturer14.30 – 15.00 Lecture 1 Introduction to Laboratory Accreditation 17025 Michael Jackman, Project Biology & Ecology Expert15.00 – 15.30 Lecture 2 Production of Standard Operating Procedures (SOPs) & Michael Jackman, Project Biology & Ecology ExpertLaboratory Information Management Systems (LIMS)Introduction to Analytical Quality Control, Precision andAccuracy15.30 – 15.45 Coffee Break15.45 – 16.15 Lecture 3 Validation of Analytical Methods & Associated Software Michael Jackman, Project Biology & Ecology Expert16.15 – 16. 30 Questions and DiscussionsKEs Mission ReportsHuman Dynamics KG ConsortiumPage 27 of 36

Appendix 7 - List of Participants at Local Quality Control & GLC Training Workshops at NEMD in Baku, Azerbaijan on 20th-21st FebruaryNo Name Position Attended training1. Ayubeniz Dushdurova Lab Monitoring Water AQC 20/2/13 & AQC Practical Training -21/2/32. Aliyeva Aytan - AQC 20/2/13 & AQC Practical Training 21/2/33. Pashayeva Resmiya - AQC 20/2/13 & AQC Practical Training 21/2/34. Madatiova Durdana Head Of Monitoring Lab On Atmosphere Precipitations AQC 20/2/13 & AQC Practical Training 21/2/35. Efendiyeva Roya Secondary Chemical Engineer AQC 20/2/13 & AQC Practical Training 21/2/36. Abdullayeva Ramina Head Of Monitoring Lab On Atmosphere Air Pollution AQC 20/2/13 & AQC Practical Training7. Aliyaeva Aytan Senior Chemical Engineer AQC 20/2/13 & AQC Practical Training 21/2/38. Ulker - AQC 20/2/139. Khalilova Aybeniz Head Of Monitoring Lab On Earth Pollution AQC 20/2/1310. Aliyeva Arzu Secondary Chemical Engineer AQC 20/2/1311. Quliyeva Sevda Secondary Chemical Engineer AQC 20/2/1312. Qasimova Ruhiyya Chemical Technique AQC 20/2/1313. Askerova Sabina Chemical Technique AQC 20/2/1314. Abulhasanova Natravan Senior Chemical Engineer AQC 20/2/1315. Latifova Shakhla Chemical Engineer AQC 20/2/1316. Allahverdiyeva Rena Senior Chemical Engineer AQC 20/2/1317. Gulkar Mammadova, Deputy Head of Laboratory AQC 20/2/13 & GLC 21/2/318. Allahverdiyeva Ragiba Secondary Chemical Engineer AQC 20/2/13 & GLC 21/2/1319. Guliyev Nizami Physical Engineer AQC 20/2/13 & GLC 21/2/1320. Mamammadova Sabina Head Of Spectrometric And Chromatography Lab AQC 20/2/13 & GLC 21/2/1321. Baghirova Gulnar Physical Engineer AQC 20/2/13 & GLC 21/2/1322. Mayilova Lala Chemical Technique AQC 20/2/13 & GLC 21/2/13KEs Mission ReportsHuman Dynamics KG ConsortiumPage 28 of 36

Appendix 8 - Main issues and recommendations for Improving the Organic Micro-pollutant Analysis at EIMC, Yerevan, ArmeniaSampling BottlesThe sampling bottles are too small, (160ml) ideally the bottles should be 2000ml amber glass with ground glass tops or caps with Teflon inserts. The reasonthese smaller bottles were used was because obtaining extraction solvents of pentane or hexane is very difficult for Armenia owing to importing problems.So it was thought that by using smaller bottles then less solvent would be required. Whilst this seems logical it must be noted that the extraction is also animportant concentrating technique. If the sample is too small then it creates a problem in being able to extract enough of the OPG from sample to be ableto detect it by GLC analysis. It is very unlikely that the current small samples with be sufficient to reach the required limit of detection (LOD).Extraction ProceduresThe extraction/concentration procedures could be a problemCurrently the liquid/liquid extractions are evaporated overnight at 60 degrees to dryness and then made up to 1ml. The extractions should not beevaporated to dryness there is a high chance that the organics will also evaporate and be lost. So the evaporation should be done to 1ml.Using this technique will also lose some of the VOCs and the aromatics. Ideally the purge and trap systems should be used – this is a GLC samplingaccessory. Also head space analysis may also be useful. Note one can use water samples and not need hexane could be useful to ArmeniaCalibration StandardsThere is one old standard for VOC (Mix of 23 parameters) which is out of date. No standard for the OCPs. Only 3 old standards for the aromatichydrocarbons. Therefore for the training new standards, are required.As there are no OCP standards available. The analyst has relied on previous calibrations obtained in 2006 by their predecessors. The only was to resolvethis, is to use fresh standards and prepare new acceptable calibration standards. It is recommended that the KE3 could train the staff to obtain suchcalibration graphs in following missions..KEs Mission ReportsHuman Dynamics KG ConsortiumPage 29 of 36

GLC SoftwareEach of the 4 GLCs has its own operating software. The staff have not been adequately trained on these, which limits the application of the software.However the important elements of the analysis can be easily obtained from the software such as:i.)The retention times.ii.)The areas and heights of the integrated peaksiii.)The operational conditions of the GLCiv.)The identification of the peaksThese elements are sufficient to carry out qualitative and quantitative analysis by using simple calibration graphs and processing this data either manuallyor with Excel Spreadsheets.This could also be useful training for the staff as they would have a clearer understanding how the results are calculated.In parallel with this, the staff could continue to improve their knowledge of the system by further investigating the software together. In addition the staffcould refer to the manufacturers, and the Slovakian Institute to assist with answering specific points.GLC Carry Over - Ghost PeaksThe Agilent GLC suffered from carry over of ghost peaks. The temperature programme has a 1 minute clean out phase at 315 ºC. The KE3 recommended toresolve the problem by applying the following modifications to the methods:i. The temperature programme had only a 1 minute clean out phase at 315 ºC. It was recommended to change the programme by extending this phaseto up to 15 minutes.ii. Increase the temperature of the inlet port.iii. Change the septa more frequently.Laboratory EnvironmentKEs Mission ReportsHuman Dynamics KG ConsortiumPage 30 of 36

This sensitive analysis needs a laboratory that is clinically clean and in pristine condition, because any dust or volatiles in the air can contaminate the results.Unfortunately the EIMC laboratories are not nearly up this standard. There is a real need for the laboratories to undergo a complete refurbishment or to berelocated to modern premises so that the analysis can be of a standard for a national environmental department.Recommended Future WorkPetroleum Aromatic Analysis1) Ensure subtraction of blanks2) Try different extraction techniques3) Try suggested Excel techniques4) Write SOP for methodOCP analysis1) Develop the KD analysis- check recoveries.2) Use higher threshold values to remove poor peaks3) Ensure there is blank correction4) Try suggested Excel techniques5) Write SOP for methodVOC analysis1) Construct calibrations curves for all standards2) Ensure there is blank correction3) Try to remove ghost peaks4) Try different extraction techniques5) Try suggested Excel techniques6) Write SOP for methodKEs Mission ReportsHuman Dynamics KG ConsortiumPage 31 of 36

Appendix 9 - Water Bodies at Risk in Pilot River Basins in ArmeniaWater Bodies at Risk from Anthropogenic activities in Akuryan RiverLocation Anthropogenic activities PointSourcePollutionNon-PointSourcePollutionPollutant ParametersImmediately downstream of Gyumri Town Urban Activities & associated wastewaters√ √ BOD, Total N, NO3, PO4, Total P,Cl, NH4, & TDSImmediately downstream of Artik Town Urban Activities & associated waste √ √ BOD, Total N, NO3, PO4, Total P,watersCl, NH4, & TDSImmediately downstream of Maralik Town Urban Activities & associated waste √ √ BOD, Total N, NO3, PO4, Total P,watersCl, NH4Immediately downstream of Karkachun Extraction of Construction Materials √ √ Na, Mg,K, Ca, Br, Mn,Sr, Mo, Sb,RiverSO4, & Cl, TDS, TSS,Karkachun River Cattle Breeding √ BOD, Total N, NO3, PO4, Total P,Cl, NH4, & TDSLengthof WB?????Table 8Water Bodies at Risk from Anthropogenic activities in Metsamor RiverLocation Anthropogenic activities PointSourcePollutionImmediately down stream of largesettlementsIn the mid to lower sectionsFish farms In the mid to lower sectionsUrban Activities & associated wastewaters & Effluent from Food industryUrban Activities & associated wastewatersNon-PointSourcePollutionPollutant Parameters√ √ BOD, Total N, NO3, PO4, Total P,Cl, NH4, & TDS√ √ BOD, Total N, NO3, PO4, Total P,NH4, TDSLengthof WB??Page 32 of 36KEs Mission ReportsHuman Dynamics KG Consortium

201020112012201020112012201020112012201020112012201020112012Appendix 10 - Ecological categories for water quality of the rivers in Akhuryan 1Quality category/indicatorUnit of Categorymeasure excellent good moderate poor badDissolved oxygen mg/l >7 >6 >5 >4 18COD- Cr mg/l 10 25 40 80 >80Ammonium ion mg N /l 0.057 0.4 1.2 2.4 > 2.4Nitrite ion mg N /l 0.007 0.06 0.12 0.3 >0.3Nitrate ion mg N /l 0.463 2.5 5.6 11.3 >11.3Orthophosphate ion mg/l 0.085 0.1 0.2 0.4 > 0.4Total phosphorus mg/l 0.086 0.2 0.4 1 >1Total mineral nitrogen mg/l 0.8 4 8 16 >16Suspended particles mg/l 25 30 50 100 >100Results of assessment of water quality in Akhuryan RBIndicatorMonitoring points*31 32 33 34 38Dissolved oxygenBOD 5COD- CrAmmonium ionNitrite ionNitrate ionPage 33 of 36KEs Mission ReportsHuman Dynamics KG Consortium

201020112012201020112012201020112012201020112012201020112012IndicatorMonitoring points*31 32 33 34 38Orthophosphate ionTotal phosphorusTotal mineral nitrogenSuspended particlesPage 34 of 36KEs Mission ReportsHuman Dynamics KG Consortium

Appendix 11 - Draft Letter from First Deputy Minister of EnvironmentPage 35 of 36KEs Mission ReportsHuman Dynamics KG Consortium

Appendix 12Project is funded by theEuropean UnionProject is implemented by the ConsortiumLed by Hulla & Co. Human Dynamics. K.G.Certificate of TrainingThis is to certify thatMs Anna AloyanParticipated in theTraining Workshop “Introduction to Quality Control for Water Laboratories & Gas LiquidChromatography”organized by the EU funded Project “Environmental Protection of InternationalRivers Basins” on 14 February, 2013 in Yerevan, Armenia”This included introductory training for:√ Standard Operating Procedures & Laboratory Information Management Systems√ Analytical Quality Control, Precision and Accuracy√ The Application of Gas Liquid Chromatography for the Analysis of Organochlorine Pesticides_ Zurab JincharadzeDeputy Team Leader of EPIPB ProjectKEs Mission ReportsHuman Dynamics KG ConsortiumPage 36 of 36

5th Mission Report _KE3_March-April 2013Table of Contents1. BELARUS (31st March – 6th April 2013) ........................................................................................................ 22. MOLDOVA (6th April – 13th April 2013) ....................................................................................................... 43. UKRAINE (21st March – 31st March 2013) ................................................................................................... 8Annex 1 - Copy of Moldova Ministry of Environment Website Publicity on the Project ....................................... 13Annex 2 - Local Training Workshop in Chisinau for Laboratory Quality Control ................................................... 15Annex 3 - Agenda for the Local Training Workshop in Chisinau for Laboratory Quality Control .......................... 16Annex 4 - Terms of Reference FOR Short Term Benthic Macro Invertebrate (BMI) Expert ................................... 17Annex 5 - Terms of Reference Short Term senior Biologist Non-Key Expert ......................................................... 19Annex 6 - Evaluation reports for NKEs .................................................................................................................. 22Annex 7 - Workplan for KE3 Mission 4 .................................................................................................................. 23Page 1 of 23

5th Mission Report _KE3_March-April 2013IntroductionFollowing the previous training missions in Eastern Europe, it was decided to progress the issues that hadbeen raised in the previous mission: to provide support and training for the laboratories in each country.This encompassed Activity 1.5 “Support the analytical quality control assurance procedure” and Activity1.6 “Assess the needs regarding laboratory infrastructure, equipment and training” by developing theinternal quality controls and the analysis of organo chlorine pesticides (OCPs) of each of the water qualitylaboratories. In addition the mission included the KE3’s input for the delineation of water bodies, activity2.2. The mission starting on 20th March and completed on 14th April according to the operational plan isshown in Annex 7.The tasks included visiting the three beneficiary countries to submit the following:1) Intensive training on the application of internal quality controls.2) Investigate the current methods and provide training for organochlorine pesticides (OCPs).3) Further discuss the water laboratories’ capabilities of sampling, analyzing benthicmacroinvertebrates and their training needs.4) Assist each laboratory with their own analytical problems that could affect their analysis for theProject.5) Submit extra local training workshops in each country tailored for their individual requirements.6) In addition to the laboratory inputs the KE3 worked with KE2, KE4 &KE5 on the delineation of waterbodies (WB) in the three countries for Activity 2.2.1. BELARUS (31ST MARCH – 6TH APRIL 2013)National Monitoring Laboratories’ TrainingTwo main authorities are involved in monitoring of surface water quality on the River Dnieper:1) Republican Centre of Analytical Control in the Field of Environmental Protection (RCACFEP))-2) Republican Centre for Radiation Control and Environmental Monitoring (RCRCEMThe following training and discussions were undertaken:The Republican Centre of Analytical Control in the Field of Environmental Protection (RCACFEP))- Ministryof Natural Resources & Environmental Protection (5 th December 2012)Present1) Vladimir Antskevich, (Director of Centre)2) Svetlana Utochkina ,(Deputy Director of Centre)3) Victoriya Zmitrovich, (Head of Laboratory)4) Alexandr Stankevich, (CMWE)5) Veronika Selitskay, (Data Processing Engineer)Laboratory AccreditationThe HQ in Minsk and the regional laboratories all apply internal quality control standards for the mainparameters. The Department is nationally accredited and the national accrediting agency is soon to beaccepted as an international accrediting agency. Therefore this current accreditation would then beupgraded to the ISO 17025 standard.Page 2 of 23

5th Mission Report _KE3_March-April 2013The method validation software submitted at the workshop initially functioned well but recently couldnot be operated. This is currently being investigated by KE3.Svetlana Utochkina was keen to visit Hydromet in Moldova and other international laboratories astraining study tours to obtain further training in accreditation, which was supported by KE3.OCP AnalysisThe Department analyses OCPs in river water on a regular basis using the ISO 6468 standard method.However, their current calibration standards from LGC are limited and they particularly require thestandard for pp DDT, which is an important analyte required by the WFD.Furthermore the Department has experience in analyzing OCPs in river sediments and has recentlycompleted a survey of the pesticides at transboundary of River Bug. However, they are not sure if theircurrent sampling sediment procedures comply with the WFD. Training will be submitted at the JFS.2) Republican Centre for Radiation Control and Environmental Monitoring (RCRCEMPresentGennady Tischikov, (Head of Hydrobiology)Natalia Kalitskay, (Head of Hydrobiology Laboratory)Nina Misukaviz, (Hydrobiologist)Tatiana Milochevskaya, (Hydrobiologist)Olga Kovalenko, (Hydrobiologist)Soja Yozelicheva, ( Hydrobiologist)Veronika Selitskay, (Data Processing Engineer)Igor Tischikov, Principal Engineer Chemist Dept for Surface Water MonitoringHydrobiological AnalysisThe Hydrobiological Department was established in 1974 and has about 6 staff. It was proposed thatduring the JFS the Senior NKE will assist and train on the hydrobiological sampling, and the KE3 will trainon the Chemical/ Physical sampling to recommended ISO standard. The KE3 and Junior NKE BMI will visitthe labs in September/October to assist in the analysis and processing of results. The JFS will only bescheduled to sample from about 30 locations, therefore the selection will be very important especially forthe WBs at risk e.g. Tiskova.Study tours were discussed and the Department recommended that the Institute of Water at Slovakia(Bratislava) would be an ideal training centre for hydrobiology and also for hydrochemistry. (Contacts: DrJarmila Makovinska & Dr Liva Tothova who worked with the Department on the Transboundary study ofthe River Bug 2002, 2009 & 2012. (EU WW/SCRE1/No))There are 88 sampling points in the Upper Dnieper for hydrochemical and biological with about 30rivers/tributaries and 11 lakes/reservoirs. The Department can analyse all hydrobiological elements, apartfrom macrophytes and fish and are also developing the method for Chlorophyll A.The overall classification of the rivers has already been done according to the 5 new categoriesestablished in Belarus, which has similarities to the WFD. This information is still being prepared and is notofficially released yet. According to the Belarus system some WBs can have different classificationsaccording to either the chemical or hydrobiological systems. This is contrary to the WFD that can onlyhave one unified classification which would grade to the lowest category.WBs DelineationPage 3 of 23

5th Mission Report _KE3_March-April 2013A workshop was held on 2nd April with GIS Delineation Team presented by KE4 and supported by KE3.PresentAliksandr V Pakhomau – Head of Sector – Central Research Institute for Complex use of Water Resources (CRICUWR)Natalia Kalitskaya, (RCRCEM Head of Hydro biology)Vladimir Koneev, Head of Monitoring & Cadastre CRICUWRKonstantin Titov, (RU/UNDP Project)Yuri Diasmichy, Hydrometerological CentreElena Bogodiazh, Researcher CRICUWRVeronika Selitskay, Data Processer RCRCEMAlexandr Stankevich, CMWEThe WFD delineation process of WBs uses system A that is based on geological, and hydromorphologicalaspects will be applied. Belarus has a similar system of delineation using water catchments but using adifferent range of values. The KE3 explained that the extra information of highlighting WBs at risk isimportant and this information should be available in the RBA.2. MOLDOVA ( 6TH APRIL – 13TH APRIL 2013)The main agency monitoring the River Prut and is the main beneficiary is the State Hydromet Services,(SHS)Present1) Gavril Gilca, Head of Environmental Quality Monitoring Department SHS2) Svetlana Stirbu, Deputy Chief of Dept. Environmental Quality Dept, SHS3) Jurcu Valentina, Head if Laboratory, Surface Water Quality Center, SHS)4) Racovet Natalia, Engineer, Surface Water Quality Centre, SHS5) Ana Kumacova, Chief of the Soil Quality Monitoring Centre, SHS6) Natalia Molnicean, Senior Chemist7) Galina Buga, Senior Chemist8) Nadejda Orlova, Senior Chemist9) Anatolie Tosan , Sampling Expert10) Valerie Donici, Sampling ExpertThe following training and discussions were undertaken with the listed staff and included the following:Laboratory AccreditationThe laboratory had an external audit undertaken by the MOLDAC, the national and internationalaccreditation authority, and the following issues were raised by this agency:A few items were considered non conformity:1) The Department must display the new regulations of ISO17025 - this seems a minor issue and easilyrectified.2) The environmental conditions of the laboratory were out of range.Page 4 of 23

5th Mission Report _KE3_March-April 2013i. The Temperature must be 20ºC, sometimes it was 18ºC, and therefore the analysis should bediscounted in such circumstances.ii. The Relative Humidity changed significantly on a daily basis owing to the sensor not set upcorrectly- this was easily rectified.3) The Carey portable spectrophotometer was faulty and no longer used for air and water analysis fornitrates, nitrites, & phosphates. The accreditation agency stated that they should have been informedabout the condition of this instrument and also informed that this analysis was no longer done. Thisproblem was also easily rectified.Analytical Quality ControlThe method validation software submitted at the workshop initially functioned well but recently couldnot be operated. This is currently being investigated by their computer expert, Vova Linik, and the KE3submitted an expert link to resolve this problem.Since the last AQC training workshop, new staff had recently been engaged and the KE3 was requested tosubmit another customized workshop to concentrate on internal AQC charts and method validations.Since the last AQC training workshop, new staff had recently been engaged and the KE3 was requested tosubmit another customized workshop to concentrate on internal AQC charts and method validations. Thestaff from the State Hydrogeological Department and the State Ecological Inspectorate were also invitedto attend. The list of all the delegates and the agenda is detailed in Annex 2 & 3. It is recommended thattraining certificates are issued to these staff by the Project.Water & Sediment SamplingAn important aspect of monitoring for the WFD is sampling. Therefore the KE3 focused on the SamplingDepartment and submitted on-site training for the sampling staff at Westmurka Reservoir, near KishnerGate at the sampling platform on 10th April. The following issues were noted:i. The pesticide bottles were not ideal, as they had large mouth, with a screw top lid. Such bottlescould suffer from high evaporation losses of volatile components and also it was difficult to avoidair being trapped in bottle, which should not be permitted.ii.iii.iv.Three bottles used preservatives: e.g. Sodium Hydroxide (NaOH) for phenol analysis, Chloroform(CHCl3) for nutrients parameters, and Sulphuric Acid H2SO4 for petroleum products. It wasrecommended that with the last two sample bottles should be washed with the sample beforeadding the reagents on site.These three bottles were 1L amber glass bottles stored un-capped. It was recommended that infuture they are stored with their caps on to prevent dust contaminating them. Also the caps hadfragile cardboard inserts, which had been damaged by the corrosive preservatives. In fact many ofthese were so damaged that the actual tin plate lid was corroding. It was recommended that allthese caps are replaced by plastic caps or with new caps with Teflon inserts.Large 2L plastic sampling containers were used to take the other water samples. These were alsonot ideal, as it was impossible to prevent air being trapped in the vessel and thus contaminating thesample. It was recommended that alternative bottles are used.v. There were four sediment samplers, two were grabbers, which appear to function well. The othertwo were core samplers- one could be operated manually and the other one could be usedPage 5 of 23

5th Mission Report _KE3_March-April 2013remotely. This latter one may only be suitable for taking marine samples. It was recommended thatall these should be investigated further in the JFS.vi.vii.One of the samplers smoked during the procedures, ideally this should not be allowed, as thesmoke particles could contaminate the samples, especially the organic ones. It is recommendedthat the samplers should be advised about this problem.Finally the KE3 commissioned a portable turbidity meter, which had never been used for 6 years.The sampling staff were trained on its operational method and afterwards obtained satisfactoryresults as NTU and FTU units.OCP AnalysisThe Chromatography Department analyse the 25 parameters recommended by the StockholmConvention in water and soil and these include the OCPs. The Department is currently using GOSTstandard methods, which give satisfactory results, however to assist with further method developmentthe KE3 submitted three other international methods as requested.The extraction phase is carried out by using a liquid/liquid extraction technique using hexane and for thesoil a similar technique but also using acetone in the hexane.The concentration phase uses a rotary evaporator. It was noted that with soil samples 1 ml of iso-octaneis added to the hexane to reduce losses from volatile components.The clean up phase uses Sulphuric Acid, as per the method, however this affected the recoveries of Aldrinand Endrin, therefore the Department now used silica gel or alumina columns.The Department previously used Isodrin as an internal standard, which was effective to calculate recoverylosses for each sample. Unfortunately, the Department has depleted this standard and it is recommendedthat this standard is obtained by the project to complete the training for the JFS.The chromatographic phase is carried out by using an Agilent GLC (7890A) and Mass Spectrometerdetector (5975C) with a DB5 –MS 30 m X 0.25 X 0.25µm capillary column.The results appear satisfactory indicated by the application of internal AQC charts.On-line Monitoring SystemsThe Department had been funded to install two online monitoring stations for Temperature, pH, EC, DO ,turbidity and level on the River Prut. Unfortunately one is no longer operational and the other cannot beused as it proved too expensive to maintain and operate. However, Hydromet believe that such systemsare important in protecting the quality of the River Prut especially in monitoring the transboundaryregions.Hydromet recently undertook a new hydrology project (funded by the Czech Republic) which included theinstallation of a number of hydrology stations to measure flows and levels along the River Prut. Thesestations consisted of bridges or platforms that suspend the sensors in the river. This system has extraspace for 18 more sensors for water quality measurements and could also include very useful sensorssuch as for petroleum products and ammonia, especially for water bodies at risk. It is recommended thatthe project investigates these monitoring points during JFS with a view to fund the renting of such sensorsat locations at WBs at risk as a trial, as a pollution control measure.Page 6 of 23

5th Mission Report _KE3_March-April 2013Early Warning and Emergency Response Systems (EWERS)The senior managers discussed with KE3 the EWERS that are used with the ICPDR (Danube Commission).Each country has dedicated contact sites and an emergency system of alerting countries of any seriouspollution upstream of the Danube, which includes some of the major tributaries. Moldova has beenalerted to a number of incidents from Ukraine and some of which may have been caused by leakage fromtheir tailing dams.In ICPDR there is a useful decision making software to categorise how serious any pollution incident is andtherefore how to deal with it. However, they do not have any simple software to track the pollutant as itflows downstream, therefore these pollution incidents are difficult to monitor. It is recommended thatthe Project considers trialling a simple software developed in the recent EU project for the Yellow River inChina that uses Google Earth as a platform, which the KE3 has experience in applying.Public Awareness (Activity 2.7)The CWME arranged a high profile meeting with the Environment Minister, George Şalaru, and ViceMinister, Valentina Tapic together with Gavril Gilca & Svetlana Stirbu, SHS Senior Managers, to discuss theProject with the CWME and KE3. This proved to be a fruitful meeting and the Minister was verysupportive of the Project and the important inputs by KE3. The details of this meeting and the Projectwere immediately downloaded on to their public website:http://mediu.gov.md/index.php/serviciul-de-presa/noutati/1294-ministrul-mediului-gheorghe-salaruimplementarea-proiectului-protectia-mediului-in-bazinele-riurilor-internationale-este-un-aport-lagestionarea-eficienta-a-resurselor-de-apaThe details are included in Annex 1Training MaterialsThe training materials were discussed with the Senior Managers to ensure that sufficient equipment andchemicals will be available to complete the training by the Key Experts for the JFS. KE3 reflected theseurgent requirements in the draft Updated Surface Water Training Report submitted to the Senior ProjectManagers.WB DelineationThe KE3 and the CMWE met with Dr. Gennadii Syrdroev Institute of Geography at the Academy ofChemistry, responsible for the GIS WB Delineation. The following points were made:i. 60 WBs had been identified according to the catchment zones and includes 17 WBs at risk owing tolarge waste water treatment works from the from the major towns. These WBs need to beprioritized according to their risks from their populations.ii. The WBs need to be coded according to the international reference systems.iii. Two major fish farms are in the pilot area but currently there is no water quality information, whichneeds to be taken into account for planning the JFS to resolve this gap.iv. Moldova had a classification system using 7 categories and this is number is currently beingreduced to 5 as in Ukraine and WFD. Hopefully the conversion of this old to new system can beapplied to assist the initial classifications for the pilot area of the Prut.v. The GIS project will be completed shortly and submitted to the Project key experts.Page 7 of 23

5th Mission Report _KE3_March-April 20133. UKRAINE (21ST MARCH – 31ST MARCH 2013)Two main authorities are involved in monitoring of surface water quality on the River Dnieper:1) Basin Management Authority of Water Resources (BUVR) Dnieper River (Vyshhorod);2) State Hydrometeorological Service of the Ministry of Emergency Situations and Chernobyl Affairs.The following training and discussions were undertaken with them:1) Dnieper (BUVR)Present1) Olga Kravtsova, Head of Laboratory2) Ivan Diemliega, Deputy Head of Dnieper BUVR3) Valetin Levchenko Head of Chromatography Dept4) Natalia Zakorchevna River Management Basin Expert5) Alexande Gulidov - TranslatorQuality ControlFor special samples, the Department analyses samples in duplicate, by two analysts and then carry out aspiked recovery test, which is a reasonable check for the results However, this extensive procedure is notfeasible for normal samples and so this was not done for all samples.KE3 provided supplementary training on the AQC procedure to incorporate the use of simple ShewhartCharts in their normal analysis. Ms Kravtsova then appreciated that this procedure was simpler thanexpected and no further software would be required. Therefore the Department would in future graduallyapply this system for a number of parameters with a view to applying this to all analyses. This will bereviewed further during the next mission by KE3.The UK method validation (AQC99) software that had been submitted at the regional workshop had beeninstalled by Department and could be operated. However all the computers’ software were upgradedrecently and unfortunately the AQC99 then could no longer function. It was suggested that a computerexpert investigates this to check whether the Windows settings were correctly set for this DOSprogramme.Organo Chlorine Pesticides (OCP) AnalysisThe OCP analysis is not normally carried out routinely, but the Department had developed the analyticalmethod using a modern GC/ECD system, with two Resek Capillary columns ( Rtx 5 -30m X o.32mm 0.5 µ)& Rtx 5 -30m X o.32mm 0.26 µ).The GOST standard method is used, Ref: 52.24.2009, using liquid/liquid extraction with hexane.Apparently the calibration is undertaken by comparing results with previous chromatograms rather thanchecking recoveries and using fresh calibration standards. The KE3 advised that for the JFS samples thisimproved procedure should be applied, and these results would be reviewed during the next mission.Unfortunately the GC computer could not be operated at the time of KE3’s visit because of a powersystem fault, so the previous results could not be assessed.The Department had individual calibration standards for a limited number of OCPs that were produced inUkraine. However the quality of these standards was questionable and the Department need to use morereliable standards produced overseas.Page 8 of 23

5th Mission Report _KE3_March-April 2013Hydrobiological AnalysisFollowing the regional AQC training workshop in Minsk, where it was noted that all the other countries’water management agencies had the facility to undertake hydrobiological analysis, Ms. Kravtsova waskeen for the Department to develop their own hydrobiological facilities. Therefore she recommended forBUVR to create at least one new hydrobiological post and also purchased a microscope for this.The ToR for this new post had been prepared, candidates had been interview, and a qualified biologisthad been provisionally selected. However the appointment had still to be ratified by the managementcommittee. In addition the Department has liaised with Olena Lietitski to obtain advice.This proposed new post is a significant milestone for the Project and BUVR and it is recommended thatthis advance should be supported by the Project such that sufficient training is provided especially insampling at the JFS and subsequent BMI identification training. Ms. Kravtsova, also requested thatanother member of staff (microbiologist) should be trained in parallel with the new hydrobiologist. Thisidea was supported by KE3 as it would ensure the sustainability for this new facility.2) State Hydrometeorological Service of the Ministry of Emergency Situations and Chernobyl Affairs;This government agency is also responsible for monitoring the Dnieper River and has supplied the Projectwith important data. Currently, there is no link with Dnieper BUVR.PresentMr Oleksandr Kosovets, Director of Central Geophysical ObservatoryMrs Hanna Doroshenko, Head of Environmental Monitoring- Air, Soil, Hydrobiology, HydrochemistryMrs Oleksandra Nikitenko, Head of HydrochemistryMrs Tatyana Kuznetsova, Head of HydrobiologyMrs Ludmila Gavrilova, Head of Physico- Chemical AnalysisThe Department has the capacity to analyse up to 41 physical/chemical parameters and all thezooplankton, including Benthic Macro Invertebrates (BMI). They have 17 water quality sampling points(Physical/Chemical) in the Upper Dnieper and 8 in the River Prut. However there is only onehydrobiological point in the River Prut.The Department has national but not international accreditation. There are twelve other regionallaboratories which monitor a total of 242 sampling points consisting of 4 different categories.OCP AnalysisThe Department analyses OCPs using a GOST method, but the GLC equipment is over 20 years old, usingpacked columns, rather than using modern capillary columns. It is doubtful that the required LOD for theWFD could be obtained using such old equipment.They had individual calibration standards for four OCPs that are produced in Ukraine, but these were ofquestionable quality.Water Bodies DelineationA number of meetings were held with the Key Experts 2, 4, & 5, Natalia and also GIS Experts:Ishuk Oleksiy, Groundwater and Dnieper Surface Water Expert, and Andreychuk Yuri, Prut Surface WaterExpert.KE3 requested that the GIS Experts should ensure that they identify the WBs at risk in their GIS maps andthis information should be available in the RBA.Page 9 of 23

5th Mission Report _KE3_March-April 2013Other IssuesWhilst in Kiev, this provided an ideal opportunity for KE3 to resolve a number of other issues:i) Senior & Junior Non Key Biological ExpertsThe TORs for these positions were finalised as shown in Annex 4 &5. It was agreed for three localcandidates for these positions to be interviewed by KE3. An evaluation form was prepared and agreedand the results are shown in annex 6.Olena Liteitski was considered the best candidate for the Junior BMI position and the senior position hasstill to be resolved.ii) River Basin AnalysisKE3 discussed the current RBA’s with KE2 of the Caucus countries to ensure all the required data for WBsat risk contains sufficient detail.iii) Team Leader MeetingThe TL held an emergency meeting to discuss the results of a high level meeting with the PDs and the EU.The minutes should be circulated later, but one of the urgent issues was to improve communication byholding weekly Skype Conferences and submitting about 5 bullet points to the EU also on a weekly basis.Summary & ConclusionsActivity 1.5The KE3 visited the three Eastern European countries providing training to the water analysisdepartments to ensure that they could establish and/or develop their own internal AQC systems.In Ukraine the KE3 provided supplementary training on the AQC procedures to incorporate the use ofsimple Shewhart Charts in their normal analysis for the BUVR Dnieper. This AQC procedure was found bythe trainees to be simpler than expected, and they realized that no further software would be required.Therefore the Department would in future gradually apply this system for a number of parameters with aview to applying this to all their analyses. This will be reviewed further during the next mission by KE3.In Belarus and the regional laboratories all apply internal quality control standards for the mainparameters. The Department is nationally accredited and the national accrediting agency is soon to beaccepted as an international accrediting agency. Therefore this current accreditation would then beupgraded to the ISO 17025 standard. The senior management in the chemical water laboratories in Minskwas keen to visit Hydromet in Moldova and other international laboratories, as training study tours, toobtain further training in accreditation, which was supported by KE3.In Moldova this training included a customised local workshop at Hydromet as after the last AQC trainingworkshop new staff had recently been engaged. The KE3 was requested to submit another workshop toconcentrate on internal AQC charts and method validations. The staff from the State HydrogeologicalDepartment and the State Ecological Inspectorate were also invited to attend. In addition the results ofthe recent external audit carried out by the accrediting agency were reviewed.It is essential that these AQC systems are established prior to the Joint Field Survey in July 2013.Most countries should now have had sufficient training to undertake this challenge. However, ifnecessary, the KE3 could provide distance training and support prior to his next visit to ensure allcountries can apply these procedures.Page 10 of 23

5th Mission Report _KE3_March-April 2013Outputs1) Extra individual training on the procedures for internal AQC was submitted in Ukraine, such that theDnieper BUVR has now committed to apply these in time for the JFS.2) An extra local AQC training course was submitted in Moldova, together with the associated trainingmaterials. It is recommended that training certificates should be issued to the participants for inclusion inMoldova’s ISO 17025 quality manual.Activity 1.6All three countries have the facilities and methods to analyse the OCPs. The application of internalstandards needs to be improved in all countries to calculate losses that occur during the extractionprocedures.Belarus and Moldova routinely analyse for OCPs not only in water but also sediments too. This isimportant for the JFS, as sediment analysis will provide much more information than from just watersamples.Some laboratories had some minor problems with their OCP analysis ,which were discussed with KE3, butit is hoped that these can be resolved before the JFS. One major deficiency in all countries that had beennoted was they do not have adequate OCP calibration standards, which are consumables required for theanalysis and for preparing the Analytical Quality Control (AQC) standards. Unfortunately, the countrieshave problems obtaining and funding for these consumables. Therefore the support of the Project toobtain these is essential, not only for this Project, but also for future analysis and surveys. In order tocomplete the training, it is proposed that the project should obtain these standards and is detailed in aseparate training report.In Moldova, the KE3 commissioned a portable turbidity meter, which had never been used for 6 years.The sampling staff were trained on its operational method and afterwards they obtained satisfactoryresults as NTU and FTU units, which should be helpful during the JFS.Outputs1) The KE3 has provided training, support and recommendations to improve the OCP analysis in eachcountry especially in the application of internal and calibration standards.2) In Moldova, the KE3 commissioned a portable turbidity meter, which should be helpful during the JFS.Activity 2.2The KE3 worked with the other Key Experts in Belarus & Ukraine in developing the delineation of thewater bodies at risk. This included assisting with a training workshop presented by KE4 in Belarus.In Moldova, KE3 discussed the WBs delineation with the local GIS expert focusing on the pollutionpressures on the River Prut, such as two major fish farms which are in the pilot area. Currently there is nowater quality information on these, which needs to be taken into account for planning the JFS to resolvethis gap.OutputsThe KE3 assisted the KE4 in the training workshop on water body delineation in Belarus and instructed theGIS staff in all countries on the information regarding WBs at risk.Activity 2.4Page 11 of 23

5th Mission Report _KE3_March-April 2013It is important that all the national water monitoring departments are fully prepared for the imminentsurface water JFS. Therefore the KE3 reviewed the chemical and hydrobiological sampling and analyticalequipment with each country and this information is detailed in the recent “Updated Surface WaterTraining Report.In Moldova Hydromet requested that a review was made by the KE3 of the Water Sampling Department.This included submitting an on-site training session at Westmurka Reservoir on 10th April. This inputproved useful and seven issues were raised and recommendations were submitted to improve theirsampling procedures and/or equipment.It was recommended that the KE3 submits a Sampling Manual to assist the samplers for taking Chemical/Physical Samples and the Senior NKE Biology draft a manual on taking hydrobiological samples, prior tothe JFS.It had been proposed that two extra NKEs for biology would be recruited especially for the JFS. The KE3interviewed three possible candidates in Kiev using agreed evaluation forms that had been prepared asshown in Annex 6. One candidate for the Junior BMI position had been recommended.Outputs1) An on site training course was submitted in Moldova on Chemical/ Physical sampling by KE3.2) Seven issues were raised and recommendations for improving procedures and equipment weresubmitted.3) The KE3 reviewed the chemical and hydrobiological sampling and analytical equipment with eachcountry and this information is detailed in the recent “Updated Surface Water Training Report”.Public Awareness ( Activity 2.7)In Moldova a high profile meeting with the Environment Minister, George Şalaru, and Vice Minister,Valentina Tapic together with Gavril Gilca & Svetlana Stirbu, SHS Senior Managers, was arranged todiscuss the Project with the CWME and KE3. This proved to be a fruitful meeting and the Minister wasvery supportive of the Project and the important inputs by KE3. The details of this meeting and the Projectwere immediately downloaded on to their public website and are detailed in annex 1.OutputFollowing a meeting between KE3 and CMWE and the Minister of Environment, an article was publishedin the Moldovan MOE website detailing to the public the information concerning the mission of KE3 andthe details of the Project.Activity 2.5 Development of national and basin-wide Programmes of Measures.Whilst the KE3 was discussing the JFS at Moldova Hydromet, the senior management were concernedabout other water quality monitoring procedures. These included on-line monitoring and Early Warningand Emergency Response Systems (EWERS), which should also be considered as a means of installingmeasures for protecting water bodies at risk. This has been planned to be addressed later in the project,but this was a useful opportunity to consider some future options for resolving some gaps that wereidentified. These options include:i.) renting water quality sensors to integrate with their new hydrology monitoring systems;ii.) investigate the application of water quality monitoring software used in other EU IRBM projectsthat the KE3 has successfully applied.Page 12 of 23

5th Mission Report _KE3_March-April 2013Annex 1 - Copy of Moldova Ministry of Environment Website Publicity on the ProjectToday, April 11, Environment Minister George Şalaru had a meeting with key expert for the project"Environmental Protection in International River Basin" Michael Jackman, who was on a mission at theState Hydrometeorological Service (SHS).Introduction to the topic for discussion was made by Deputy Valentina Tapis, who said that project isfunded by the European Union under the "European Neighbourhood Policy Initiative (ENPI) countries inEastern Europe and Russia" and implemented by Hull & Hyman Dynamics Consortium during 2012-2015. It aims to protect and efficient management of water resources in international river basins,especially in the Black Sea region as a continuation of activities started earlier in the international projectsimplemented in the Black Sea region. Key Expert, Michael Jackman, explained to the Minister Gh.Şalaruthat the implementation of the project included visiting the State Hydrometeorological Service (SHS), withthe task of investigating the laboratories of the Department of Environmental Quality Monitoring SHS. Hewas focusing on with the analysis of chlorinated pesticides in surface waters and international standardmethods for the examination of the application subsequent analysis of the parameters set.Page 13 of 23

5th Mission Report _KE3_March-April 2013This task is descended from the need revision procedures and sampling methods for the analysis ofphysico - chemical, including sediment sampling with field trips to assess and train staff of SHSsample. Important will be the conclusion of the evaluation of newly identified non-compliances andrecommendations by the International Accreditation System Audit Centre for Surface Water QualityMonitoring of SHS, the only internationally accredited laboratory in the beneficiary countries of theproject.Minister for the Environment George Şalaru suggested that all the recommendations mentioned in thefinal report of the mission to be taken into consideration in the preparation of joint expedition Prut riverbasin to collect additional information in the development of river basin management plan selectedinternational cross border by beneficiary countries accordance with the requirements of the EU WaterFramework 2000/60/UE. Expert Michael Jackman said that suggestions are welcome and they will bediscussed at a seminar entitled "Quality Control System" on April 12 at SHS specialists directly claimedEnvironmental Quality Monitoring, Hydrogeology Expedition EHGeoM and State Ecological Inspectorate,the staff involved in the management of Prut Basin Monitoring.Page 14 of 23

5th Mission Report _KE3_March-April 2013Annex 2 - Local Training Workshop in Chisinau for Laboratory Quality ControlParticipants listNr.ord.Name, surname Function, Organisation Contacts1. Mihni OlgaEngineer, Physico-Chemical AnalysisCenter , SHS0692585572. Josan Anatolie Engineer, The Expedition Group, SHS +373 227624663. Ghies NataliaEngineer, Ambient Air Qualityairpollution2007@yahoo.comMonitoring And Radioactive+373 22762566Background Level Center, SHS4. Cumanova AnaChief of the Soil Quality Monitoring+373 22795653105. Racovet NataliaCenter, SHSEngineer hydrobiology, Surface WaterQuality Monitoring Center, QualityManager, SHS6. Stirbu Svetlana Deputy Chief of EQMD, SHS7. Rusu LudmilaEngineer, Surface Water QualityMonitoring Center, Quality Manager,SHS8. Trafail Silvia Engineer chemist, “EHGeoM”9. Bujac Victor ERIRB CWME10. Michael Jackman ERIRB Key Expertnataliaracovet1901@yahoo.com+373 22762466stirbus@mail.ru+373 78906020Lduda@mail.ru+373 22762466Trafailsv@gmail.com+373 68472309Page 15 of 23

5th Mission Report _KE3_March-April 2013Annex 3 - Agenda for the Local Training Workshop in Chisinau for Laboratory Quality ControlTechnical Assistance & Capacity BuildingCourseLaboratory Quality ControlsDate12 th April 2013 (0.5 day)ObjectivesRevision of the procedures and techniques required for Laboratory Quality ControlsTarget groupStaff involved in River Basin Management MonitoringMaterialsComputer PowerPoint Presentations with Projector, Flip Charts, supporting information and softwareLocationHydromet, Chisinau, MoldovaDAY 1Time Method Content Lecturer10.00 – 10.15 Introduction Hydromet Senior Manager & Victor Burjac(Project Country Water Manager)10.15 – 10.30 Lecture 1 Production of Standard Operating Procedures (SOPs)) Michael JackmanBiological & Ecology Expert10.30 – 11.00 Lecture 2 Introduction to Analytical Quality Control, Precision , Accuracy &Shewhart Charts11.00 – 11.15 Coffee Break11.15 – 11.30 Lecture 3 Demonstration and discussion of Internal AQC systems used in UK &HydrometMichael JackmanBiological & Ecology ExpertMichael JackmanBiological & Ecology Expert11.30 – 12.00 Lecture 4 Validation of Analytical Methods Michael JackmanBiological & Ecology Expert12.00 – 12.15 Lecture 5 Practical Demonstration & Discussion of Method Validation Softwareused by UK and HydrometMichael Jackman &Hydromet Lab Staff12.15 – 12.30 Questions & Discussion Victor Burjac (Project Country Water Manager)Page 16 of 23

5th Mission Report _KE3_March-April 2013Annex 4 - Terms of Reference FOR Short Term Benthic Macro Invertebrate (BMI) ExpertShort Term Benthic Macro Invertebrate (BMI) Expert for: Activity 1.3 Develop WFD-compliantmonitoring programmes, including hydro-biological elements,I. Background and general objectivesA major part of the water quality monitoring required by the Water Framework Directive (WFD) includesup to five different biological elements, which include the assessment of Benthic Macro Invertebrates(BMI). All the six project countries require assistance to develop and improve their procedures forsampling, identifying and to process this BMI data to calculate the Ecological Quality Ratios (EQR) in thewater bodies in the pilot areas.Reviews have been made of the status of the BMI monitoring of each of the six project countries by theEcology & Biological Key Expert 3 (KE3). It has been noted that the water management agencies in thepilot areas in the six countries are at differing levels of expertise and so require differing support- fromUkraine, which has a very limited level of BMI experience, to Belarus, which has over 30 years ofexperience.Therefore the BMI Expert will be expected to tailor the support to the individual needs of each country.However, the main priority is to ensure that each country has at least sufficient expertise to identify theBMI ‘s to species level and to calculate the biotic indices in the water bodies in the pilot areas. Finally, theBMI Expert must ensure that each country’s results are consistent with their transboundary projectneighbours. This will cover the following activities:The assignments will contribute to the implementation of the Activity 1 - Increase capacities forrespective national authorities for hydro-biological, chemical and hydro-morphological monitoring ofwater quality, including groundwater, specifically the following sub-tasks under the Result-1 of theProject Terms of Reference: Activity 1.3 - Develop WFD-compliant monitoring programmes, includinghydro-biological elements; assisting the KE3 in training and development of BMI monitoring as a keycomponent for the introduction of hydrobiological monitoring in Belarus, Moldova and Ukraine.II.Specific objectives and scope of workThe specific objectives and scope of work of the BMI Expert with this assignment will be assisting the KE3in training and development of BMI monitoring in the following tasks as parts of the above-mentionedActivities.The main tasks are to train the six beneficiary countries to:1) To identify BMIs, ideally to species level.2) Calculate the biotic IndicesFinally produce a Guidance Documents for the above.III.Specific Activities(August – October 2103)Page 17 of 23

5th Mission Report _KE3_March-April 2013Visit each beneficiary to:1) To finalise the identification of the BMIs to species level.2) Train on the calculation of the biotic indices of the water bodies.3) Produce a guidance document for identification of the BMIs, & calculation of the biotic indices.IV.Required expertiseUniversity degree in Environmental Science or relevant discipline.Post-graduate university qualification in monitoring benthic macro invertebrates or equivalent.At least 5 years experience in monitoring river water benthic macro invertebrates.Fluent in Russian, and working knowledge of English is an asset.Knowledge and work experience of the EU Water Framework Directive (WFD).Preferably work experience in former Soviet Union countries.Experience in submitting training courses on monitoring river water benthic macro invertebrates.V. Duration and scheduleAs this is a multi- country project, the BMI expert will be expected to work in each of the six projectcountries. The estimated time required for the first year will be up to 36 working days to commence inAugust 2013 .i. Training in the identification of BMIs.ii. Training in the calculation of Biotic Indicesiii. Identifying any gaps.iv. Recommendations for resolving these gaps.v. Draft Guidance Manual for Sampling & identification of the BMIs, calculation of the biotic indicesPage 18 of 23

5th Mission Report _KE3_March-April 2013Annex 5 - Terms of Reference Short Term senior Biologist Non-Key ExpertShort Term Other Biologist Non-Key Expert(s) for: Activity 2.2 - Water body identification and typology;Activity 2.3 - Analysis of baseline situation, Activity 2.4 - Joint field surveys to cover the gaps.I. Background and general objectivesThese assignments will contribute to the implementation of the Activity 2 - Development of joint RiverBasin Management Plans (RBMPs) for selected river basins, specifically to accomplish the following subtasksunder the Result-2 of the Project Terms of Reference: Activity 2.2 - Water body identification andtypology; Activity 2.3 - Analysis of baseline situation, including preliminary classification of water bodiesbased on available data; Activity 2.4 - Joint field surveys to cover the gaps, including survey designmanuals.Identifying and determining type of the water bodies in the selected pilot basins shall become the core ofthe WFD approach for the project. After identifying water bodies, defining current state of play andtypology for each identified water object, the Programme of Measures will be developed to address thosewhere the ‘good’ state is threatened.A preliminary identification of the water bodies will be undertaken using the guidelines proposed inAnnex II of the WFD and the relevant WFD Common Implementation Strategy guidelines. The first step inthis process will be determining the geographical extent of these water bodies based on all relevantconsiderations including but not limited to river types, human impacts, protected areas and use of thewater bodies. For a simpler typology System-A, based on Eco-regions as specified in Annex XI of the WFD,shall be used for the project pilot basins. This will result in identifying basic water bodies, but that whereadditional information is available and where the resources of the project and beneficiaries allow, furthersub-division of the water bodies will be considered. Final specification of identified water bodies shall beendorsed by the beneficiaries as it will involve some further actions to implement Program of Measures inthe selected basins.Analysis of baseline situation shall be conducted for identified and typed water bodies. Therefore, thisactivity will undertake preliminary classification after identification of water bodies to describe ecologicalconditions and identify ecological/biological, hydro-morphological and physico-chemical classes for eachsegment of water bodies, as well as ecological potentials for heavily modified water bodies. And finally, tocover the gaps discovered after identification of water bodies, topological description and initialclassification, the project will conduct joint field surveys in each selected pilot basin. This will allow fillingin the gaps with respect to reference conditions, as well as baseline data required for final classification ofthe water bodies and establish type-specific reference conditions wherever these are not available.II.Specific objectives and scope of workThe specific objectives and scope of work of the Other Biologist Non-Key Expert (s) with this assignmentwill be assisting country water teams and the project Key Experts in fulfilling the following tasks as partsof the above mentioned Activities 2.2, 2.3, 2.4:1. Identify and describe water body types in each of the pilot basins / sub-basins, taking into accountthe general characteristics of the River Basins based on the biological parameters;2. Define typology of the water bodies based on the Eco-regions approach of the WFD (Type-A) witha possibility of further sub-division, when possible, and the final output of full description andPage 19 of 23

5th Mission Report _KE3_March-April 2013characterisation (typology) of each of the preliminarily identified water bodies with respect to itsabiotic factors;3. Assist the team for baseline analysis of the pilot basins, specifically in describing the ecologicalconditions / potential of each water body based on available data;4. Describe the ecological class of each water body, and the ecological potential of heavily modifiedwater bodies - compliant to the EU WFD methodology and in conjunction with the data obtainedthrough the hydro-morphological classification;5. Assist the project team for identifying gaps in classifying water bodies, having insufficient data fordetermining reference conditions, etc.;6. Assist the project team to fill in the gaps with respect to reference conditions as well as baselinedata required to classify the water bodies;7. Assist the project team in producing a survey design manual for the joint field surveys in eachpilot basin, especially focusing on the hydro-biological and physico-chemical parts of the survey;8. Ensure fulfilment of the joint field survey goals with respect of hydro-biological and physicalchemicalparts of monitoring programs as it will be presented in the survey design manuals;9. Conduct specific training sessions on hydro-biological monitoring both before and during the jointfield surveys for appropriate beneficiary institutions of each pilot basin;10. Assist the project team and the beneficiary institutions to establish the inter-calibration baselinesfor the biological and hydro-morphological parameters to ensure that the information fromshared rivers (where applicable) is compatible;11. Assist the project team and the beneficiary institutions to outline and update the pressures andthreats impacting on the ecological status of each water body of the pilot basins.Qualifications and skills University degree in Biology, Ecology, or equivalentGeneral professional experience Experience in international projects related to Integrated Water management; General experience demonstrating an understanding of transposition, implementation and/orenforcement of the EU environmental legislation, particularly the Water Framework Directive,and of capacity building challenges faced by water authorities in the beneficiary countries or newEU Member or accession States;Specific professional experience At least 10 years of demonstrable experience and a proven track record in technical, scientific andsocio-economic fields required for the implementation of the Water Framework Directive andother related Directives;At least 5 years of experience specialised in biological monitoring and assessment, and river basincharacterisation/analysis;Experience in development of measures for water pollution reduction and improving ecologicalstatus;Page 20 of 23

5th Mission Report _KE3_March-April 2013Experience in development of typology of water bodies and definition of reference conditions asrequired by the Water Framework Directive;Experience in working in the beneficiary countries or/and new EU Member or accession States.III.Duration and scheduleThis assignment will assist the project team to address hydro-biological and physical-chemical parts of thedescribed above sub-tasks under the Activity 2 in six beneficiary countries. As far as the pilot basins arelocated in two distinctive regions - Eastern Europe and the Caucasus and thus their starting conditionswith respect of hydro-biological and physical-chemical data availability and monitoring practices aredifferent, hiring more than one expert for the assignment is envisaged, respectively covering pilot basinsin the Caucasus sub-region: Chorokhi-Adjaristkali in Georgia, Central Kura in Azerbaijan and Akhurian-Metsamor in Armenia; and two transboundary pilot basins in the Eastern Europe: the Prut betweenUkraine and Moldova and the Upper Dnieper between Belarus and Ukraine. This assignment will start inMarch 2013 and will last until end of July 2014. Distribution of working days per tasks and the sub-regionswill be the following:South Caucasus: 60 daysActivity 2.2 - Water body identification and typology: 15 daysActivity 2.3 - Analysis of baseline situation: 15 daysActivity 2.4 - Joint field surveys to cover the gaps: 30 daysEastern Europe: 75 daysActivity 2.2 - Water body identification and typology: 20 daysActivity 2.3 - Analysis of baseline situation: 25 daysActivity 2.4 - Joint field surveys to cover the gaps: 30 daysIV.Deliverables• Reports on typology of water bodies;• Reports outlining the preliminary classification of water bodies;• Report describing the Ecological and physico-chemical class of each water body, and the ecologicalpotential of heavily modified water bodies; Reports outlining the gaps in hydro-biological and physico-chemical data preventing meaningfulclassification of water bodies; A survey design manual, outlining the sites, parameters and water bodies to be sampled for hydrobiological,as well as identifying the equipment required for the joint field surveys; Updated water body classification for each basin / sub-basin Updated report outlining the pressures and threats driving the impacts on the ecological conditions ofeach water body (in relevant field of study)Page 21 of 23

Annex 6 - Evaluation reports for NKEsEvaluation of Senior NKE BiologistName or number TypologyExpEQRExpBioticIndicesExpHydrobiologicalMonitoringChemicalMonitoringSamplingProgrammesBaselineAnalysis expClassification ofWBSergeyAFANASYEV8 9 9 9 8 8 8 9 9 9 10 8 10 10 1 10 119.7Svetoslav DimitroCHESHMEDJIEV0.0Top Score 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 142.0Weight Factor 0.8 1 1 1 0.5 0.8 0.8 1 0.8 1 0.8 1 0.8 0.8 0.9 0.9DefiningReferenceConditionRegional expEvaluation Junior BMI ExpertName or number EQRExpBioticIndicesHydrobiologicalMonitoringSamplinmgProgrammesBMIIdenticationAquaBiobaseRegionalexpEducationWFDExpTrainingExpNo.ofLanguageEnglishLanguageRussianTotalscoreExpto Species ExpyrsLevelexpOlena Lietitska 8 8 9 9 9 9 9 9 9 9 8 1 10 151.1Yuri Sanzhak 7 7 8 8 8 1 8 9 7 8 7 1 10 119.5Top Score 10 10 10 10 10 10 10 10 10 10 10 10 10 186.0Weight Factor 1 1 1 0.8 1.2 1.2 1 0.8 1 0.8 0.8 0.9 0.9EducationWFDExpTrainingExpNo.of yrsexpLanguageEnglishLanguageRussianTotalscoreKEs Mission ReportsHuman Dynamics KG ConsortiumPage 22 of 23

Annex 7 - Workplan for KE3 Mission 4UkraineUkraine 22-Mar Kiev: DTL, KE3, KE4, KE5 work separately with local UA experts on groundvated and surface water body identification.Ukraine 23-Mar Kiev/travel: team meeting to summarise results achieved so far. KE4 and KE5 depart to homebase at 19:40, DTL departsto homebase at 20:40.Ukraine 24-Mar Kiev: weekend.UkraineUkraineUkraineUkraineUkraineUkraine 30-Mar Kiev: KE3 works in the KPO on reportsUkraine 31-Mar Travel/Minsk: KE3 departs from Kiev to Minsk by train at 21:06. KE4 departs by plane from Riga at 14:10 and arrives in Minsk at 15:20.21-Mar Travel/Kiev: TL, RBME, KE4, KE5 arrive in Kiev by train at 07:59. DTL arrives in Kiev by plane at 08:00. KE3 arrive in Kiev by plane at 13:15. TL, DTL, RBME, KE4, KE5 travel t25-Mar Kiev: KE3 continues with water body identification focusing on bodies at risk at Kiev and also works with Laboratory to improve internal AQC procedures and progress the26-Mar Kiev: KE3 works at water body identification issues focusing on bodies at risk at Kiev and also works with Laboratory to improve internal AQC procedures and progress the27-Mar Kiev: KE3 works at water body identification issues focusing on bodies at risk at Kiev and also works with Laboratory to improve internal AQC procedures and progress the28-Mar Kiev: KE3 works at water body identification issues focusing on bodies at risk at Kiev and also works with Laboratory to improve internal AQC procedures and progress the29-Mar Kiev: KE3 works at water body identification issues focusing on bodies at risk at Kiev and also works with Laboratory to improve internal AQC procedures and progress theBelarusBelarusBelarusBelarusBelarusBelarus 06-Apr Minsk/travel: KE3 departs from Minsk by plane at 06:45. KE4 departs to homebase at 13:55.01-Apr Travel/Minsk: KE3 arrive in Minsk at 08.35. KE3 and KE4 work with local experts on water body identification, including Hydrobiological department and Laboratories to i02-Apr Minsk: KE3 and KE4 work with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC03-Apr Minsk: KE3 and KE4 work with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC04-Apr Minsk: KE3 and KE4 work with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC05-Apr Minsk: KE3 and KE4 work with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQCMoldova 06-Apr Travel/Chisinau: KE3 departs from Minsk by plane at 06:45 and arrives in Chisinau at 12:35.Moldova 07-Apr Chisinau: weekendMoldovaMoldovaMoldovaMoldovaMoldovaMoldova 13-Apr Travel/Homebase: KE3 departs to homebase at 17:55.08-Apr Chisinau: KE3 works with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC09-Apr Chisinau: KE3 works with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC10-Apr Chisinau: KE3 works with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC11-Apr Chisinau: KE3 works with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQC12-Apr Chisinau: KE3 works with local experts on water body identification, including Hydrobiological department and Laboratories to improve internal AQCKEs Mission ReportsHuman Dynamics KG ConsortiumPage 23 of 23