IC Columns

IC SectionIC Columns .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..304-316Introduction .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..305-306Allsep Anion Columns .. .. .. .. .. .. .. .. .. .. .. 307Novosep Anion Columns .. .. .. .. .. .. .. .. .. .. 308Other Anion Columns .. .. .. .. .. .. .. .. .. ..309-310Universal Cation Columns .. .. .. .. .. .. .. .. .. .. 311Cation & Organic Acid Columns .. .. .. .. ..312-314Transition Metal Columns .. .. .. .. .. .. .. .. .. .. 314Surfactant Columns .. .. .. .. .. .. .. .. .. .. .. .. .. 315Guard Cartridges .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 316IC Accessories .. .. .. .. .. .. .. .. .. .. .. .. ..317-321Starter Kits .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 317IC SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 318Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..319-320EZ-LUTE Buffers .. .. .. .. .. .. .. .. .. .. .. .. .. .. 320Other Accessories .. .. .. .. .. .. .. .. .. .. .. .. .. .. 321IC Instruments .. .. .. .. .. .. .. .. .. .. .. .. ..322-327Suppressors .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..322-324Conductivity Detector .. .. .. .. .. .. .. .. .. .. .. .. 325Column Heater .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 325IC Detection Systems .. .. .. .. .. .. .. .. .. ..326-327304

IC Columns IntroductionI C C O L U M N SIC Column SpecificationsCOLUMN COMPOSITION PH RANGE APPLICATIONSAnion Exchange ColumnsAllsep Allsep A-2Novosep A-1Novosep A-2Anion/SAnion/RPRP ® -X100AN1 Cation Exchange ColumnsUniversalCationUniversalCation HRPRP ® -X200Organic Acid ColumnsPRP ® -X300Prevail OrganicAcidOA and IOAAnion ExclusionSpecialty ColumnsTransitionMetalSurfactant/RSurfactant C8Surfactant C18polymer-based anionexchanger, 7µmpolymer-based anionexchanger, 7µmpolymer-based anionexchanger, 7µmpolymer-based anionexchanger, 5µmsilica-based anionexchanger, 10µmpolymer-based anionexchanger, 10µmpolymer-based anionexchanger, 10µmpolymer-based anionexchanger, 9µmsilica-based cationexchanger, 7µmsilica-based cationexchanger, 3µmpolymer-based cationexchanger, 10µmpolymer-basedsulfonated cationexchangersilica-based reversedphase, 3µm and 5µmpolymer-basedcation exchanger,6.5µm, 8µm, and 9µmpolymer-based cationexchanger, 10µmsilica-based reversedphase, 3µm and 7µmpolymer-basedreversed phase, 7µmsilica-based reversedphase, 5µmsilica-based reversedphase, 5µmpH 2-10pH 2-11pH 2-11pH 3-12inorganic anions, weak and strong acidions, metal complexes, organic acidsinorganic anions, organic acids,suitable for both weak and stronganions in a single runinorganic anions, weak and strongacid anions; ideal for separation ofhydrophobic anionsinorganic anions and oxyhalides; idealfor separation of 7 common anions plus3 oxyhalide anions in one runEPAMETHODS300.0,Part A300.0,Part AOPTIMIZED FOR SUPPRESSEDOR NON-SUPPRESSEDCONDUCTIVITYPAGEboth 307both 307314.0 suppressed 308300.0,300.1,317.0,326.0suppressed 308pH 2-5.5 inorganic anions non-suppressed 309pH 2-12pH 1-13pH 2-13pH 2-7pH 2-7pH 1-13pH 1-13inorganic anions, weak and strong acidionsinorganic anions, weak and strong acidionsinorganic anions, weak and strong acidions, organic acidsgroups I and II cations, amines,divalent transition metalsgroups I and II cations, amines,divalent transition metals; smallerparticle size for improved peakresolutiongroups I and II cations (separate runs),amines, lanthanidessimple aliphatic carboxylic acids andalcoholsnon-suppressed 309non-suppressed 310both 310300.7 both 311300.7 both 311non-suppressed 312non-suppressed 312pH 1-9 organic acids N/A 313pH 1-14organic acids, aromatic acids, sugars/organic acids/alcohols in one run;multiple particle sizes availablenon-suppressed 313pH 1-13 organic acids, weak acid anions non-suppressed 314pH 2-7pH 1-14high capacity, separates divalenttransition metals and metal-cyanocomplexes more selectively than acation exchange columnanionic and cationic surfactants;separate short and long chainsurfactants in one gradient runnon-suppressed 314both 315pH 2-7 short chain anionic surfactants non-suppressed 315pH 2-7long chain or aromatic anionicsurfactantsnon-suppressed 315306© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.

I C C O L U M N SAnionsAlltech Anion ColumnsAllsep Anion IC Columns• Resolves fluoride away from the water dip• Meets requirements for U.S. EPA Method 300.0 part AThe Allsep Anion Column is compatible withcommon IC mobile phases: carbonate, bicarbonate,p-hydroxybenzoic acid, phthalic acid, succinic acid, andsodium octane sulfonate.Use this column for performing EPA Method 300.0Part A “Determination of Inorganic Anions in Water” perU.S. EPA guidelines.Allsep A-2 Anion IC Columns• Separates both weak and strong acid anions in onerun• Resolves formate away from fluoride and chlorideThe Allsep A-2 Anion Column separates both weaklyretained organic and strongly retained inorganic anionsin one run. The high capacity Allsep A-2 Anion Columnis also suitable for the separation of chlorite andchlorate.Resolves Fluoride Awayfrom the Water DipOrganic and Inorganic Anions1. Fluoride2. Chloride3. Nitrite4. Bromide5. Nitrate6. Phosphate7. Sulfate130331. Fluoride (1.0ppm)2. Acetate (1.0ppm)3. Formate (1.0ppm)4. Chlorite (1.0ppm)87475. Chloride (2.0ppm)6. Nitrite (2.0ppm)7. Bromide (2.0ppm)8. Chlorate (5.0ppm)9. Nitrate (2.0ppm)10. Phosphate (3.0ppm)11. Sulfate (3.0ppm) Column: Allsep Anion, 7µm, 4.6 x 100mm(Part No. 51207)Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3Flow Rate: 1.2mL/minDetector: Suppressed ConductivityColumn: Allsep A-2 Anion, 7µm, 4.6 x 100mm(Part No. 51219)Mobile Phase: 2.1mM NaHCO3/1.6mM Na2CO3Flow Rate: 2.0mL/minDetector: Suppressed ConductivityAllsep Anion SpecificationsComposition:Methacrylate based w/quaternaryammonium functional groupsParticle Size: 7µmMobile Phase Limits: pH 2-10, 0-100% organic modifierAllsep A-2 Anion SpecificationsComposition:Methacrylate based w/quaternaryalkanol amine functional groupsParticle Size: 7µmMobile Phase Limits: pH 2-11, 0-100% organic modifierALLSEP ANION COLUMNSALLSEP A-2 ANION COLUMNSPACKINGI.D. X LENGTHPEEKPART NO.SSPART NO.Allsep Anion 4.6mm x 30 51217 512164.6mm x 50 51213 512144.6mm x 100 51207 512004.6mm x 150 51209 51208PRICEPACKINGI.D. X LENGTHPEEKPART NO.SSPART NO.Allsep A-2 4.6mm x 50 51221 512204.6mm x 100 51219 51218PRICENeed an All-Guard holder? Save money with ICAll-Guard Kits on page 316.GUARDS FOR ALLSEP AND ALLSEP A-2 COLUMNSDESCRIPTION QTY. PART NO. PRICEGA-1 Anion Cartridges 3 38108All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler included .© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.307

AnionsI C C O L U M N SAlltech Anion ColumnsNovosep A-1 Anion IC Columns• Separates common anions and strongly retainedhydrophobic anions• Meets the requirements of U.S. EPA Method 314.0The Novosep A-1 Column is your best choice forroutine anion analysis and for determining other stronglyretained hydrophobic anions that previously requiredspecialty columns.The Novosep A-1 Column can simultaneously separateweakly and strongly retained anions by gradient elution.Carbonate/bicarbonate gradients offer a powerful toolfor separating anions that have a wide range of affinitiesfor the column.Novosep A-2 Anion IC Columns• Separates seven inorganic anions and three oxyhalideanions in a single run• Meets the requirements of U.S. EPA Methods 300.0,300.1, 317.0, and 326.0The Novosep A-2 Anion Column is ideal for theseparation of 10 inorganic anions by suppressor-basedion chromatography using U.S. EPA Method 300.1. Thecolumn can also be used to determine inorganic anionsin drinking water per U.S. EPA Method 300.0 and tracebromate in drinking water using U.S. EPA Methods 317.0and 326.0.Weakly and Strongly Retained Anions9299Anions in High Ionic Strength Waterby EPA Method 300.0101211 20 41. Acetate (5ppm)2. Chloride (1ppm)3 5648 12 16 20 24 Min.Column: Novosep A-1, 7µm, 4.6 x 100mm(Part No. 51406)Mobile Phase: A: Water B: 10mM Sodium BicarbonateC: 10mM Sodium Carbonatewith 1mM CyanophenolGradient: Time: 0 5 10 15 20 20.1%B: 17 28 50 0 0 17%C: 0 22 50 100 100 0Flow Rate: 1.5mL/minDetector: Suppressed Conductivity73. Phosphate (5ppm)4. Sulfate (1ppm)5. Oxalate (5ppm)6. Perchlorate (5ppm)7. Citrate (5ppm)1. Fluoride2. Chlorite Column: Novosep A-2 Anion, 5µm, 4.0 x 250mm(Part No. 51410)Mobile Phase: 3.6mM NaHCO3Flow Rate: 0.8mL/minDetector: Suppressed Conductivity 3. Bromate4. Chloride5. Nitrite-N6. Bromide7. Chlorate8. Dichloroacetate9. Nitrate-N10. Phosphate-P11. SulfateNovosep A-1 Anion SpecificationsComposition: Methacrylate based w/quaternaryammonium functional groupsParticle Size: 7µmMobile Phase Limits: pH 2-11, 0-100% organic modifier(methanol, acetonitrile, acetone)Novosep A-2 Anion SpecificationsComposition: Polyvinyl alcohol based w/quaternaryammonium functional groupsParticle Size: 5µmMobile Phase Limits: pH 3-12, 0-100% organic modifier, 10%modifier is practical upper limitPACKINGNOVOSEP A-1 ANION COLUMNSI.D. X LENGTHPEEKPART NO.SSPART NO.Novosep A-1 4.6mm x 30 51401 514024.6mm x 50 51403 514044.6mm x 100 51405 514064.6mm x 150 51407 51408PRICEPACKINGNOVOSEP A-2 ANION COLUMNI.D. X LENGTHPEEKPART NO.Novosep A-2 4.0mm x 250 51410GUARDS FOR NOVOSEP AND NOVOSEP A-2 ANION COLUMNSPRICEDESCRIPTION QTY. PART NO. PRICEGA-1 Anion Cartridges 3 38108All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler included.308© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.

I C C O L U M N SAnionsAlltech Anion ColumnsAnion/S IC Columns• Silica-based for symmetrical peak shapes• Separates inorganic and organic anionsThe Anion/S Columns are best suited for routineseparations of chloride, bromide, nitrate, and sulfate.Not suitable for fluoride analysis.EDTA andOther Anions8284Anion/R IC Columns• Polymer-based for broad pH stability• Separates seven common inorganic anionsBecause of their high pH stability, these column areideal for the separation of anions using high pH mobilephases such as p-hydroxybenzoate, sodium hydroxide,and sodium carbonate/bicarbonate. The larger dimensioncolumns (150mm and 250mm) are suitable for a highresolution separation of the seven common inorganicanions.Weak Acid Anions56971. Nitrite2. EDTA3. Nitrate4. Bromide 1. Borate (5ppm)2. Silicate (10ppm)3. Formate (10ppm)4. Sulfide (10ppm)5. Chloride (20ppm)6. Cyanide (20ppm) Column: Anion/S, 10µm, 4.6 x 100mm(Part No. 269012)Mobile Phase: 1.5mM Sulfuric AcidFlow Rate: 1.0mL/minDetector: UV at 210nmColumn: Anion/R, 10µm, 4.1 x 250mm(Part No. 269029)Mobile Phase: 4mM Sodium Hydroxide/0.1mM Sodium BenzoateFlow Rate: 1.5mL/minDetector: ConductivityANION/S SPECIFICATIONSComposition:Silica w/quaternary ammonium ionexchangerParticle Size: 10µmExchange Capacity: 0.25meq/gMobile Phase Limits: pH 2-5.5, 0-100% organic modifierANION/R SPECIFICATIONSComposition:Poly(styrene-divinylbenzene)TrimethylammoniumParticle Size: 10µmExchange Capacity: 0.19 ± 0.02meq/gMobile Phase Limits: pH 2-12, 0-100% organic modifierANION/S COLUMNSANION/R COLUMNSPACKINGI.D. X LENGTHPEEKPART NO.SSPART NO.Anion/S 4.6mm x 100 269012 2690134.6mm x 250 269011 269001PRICEPACKINGI.D. X LENGTHPEEKPART NO.SSPART NO.Anion/R 4.1mm x 100 – 2690314.1mm x 250 – 2690294.6mm x 100 269036 –4.6mm x 150 269034 –PRICENeed an All-Guard holder? Save money with ICAll-Guard Kits on page 316.© Copyright 2004 Alltech Associates, Inc.GUARDS FOR ANION/S AND ANION/R COLUMNSDESCRIPTION QTY. PART NO. PRICEGA-1 Anion Cartridges 3 38108All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler included.Contact your Alltech office or distributor for current or local prices.309

I C C O L U M N SCationsAlltech Cation ColumnsUniversal Cation and Universal Cation HR IC Columns• Separate Groups I and II cations in one isocratic run• Separate transition metals without post-columnreaction• New Universal Cation HR 3µm particle size forimproved efficiencyCompatible with a variety of mobile phasesincluding complexing acids (citric acid, tartaric acid,oxalic acid), mineral acids (nitric acid, hydrochloricacid, sulfuric acid) and non-complexing organic acids(methanesulfonic acid).1. Lithium (0.12ppm)2. Sodium (0.90ppm)3. Ammonium (0.90ppm) Cesium and Strontium130354. Potassium (1.5ppm)5. Cesium (0.8ppm)6. Magnesium (1.2ppm)7. Calcium (1.2ppm)8. Strontium (1.6ppm)Metal Ions1. Lithium (0.2ppm)2. Sodium (1.5ppm)3. Ammonium (1.5ppm)4. Potassium (2.5ppm)103185. Nickel (2.5ppm)6. Zinc (2.5ppm)7. Cobalt (2.5ppm)8. Magnesium (2.0ppm)9. Calcium (2.0ppm) Column: Universal Cation, 7µm, 4.6 x 100mm(Part No. 27106)Mobile Phase: 3mM Methanesulfonic AcidFlow Rate: 1.0mL/minDetector: Conductivity Rapid Analysis of Monovalentand Divalent Cations13013 Column: Universal Cation, 7µm, 4.6 x 100mm(Part No. 27106)Mobile Phase: 2mM Tartaric Acid/1mM Oxalic AcidFlow Rate: 1.0mL/minDetector: Conductivity2341561. Lithium2. Sodium3. Ammonium4. Potassium5. Magnesium6. CalciumUniversal Cation SpecificationsComposition:Silica coated with polybutadiene/maleicacid copolymerParticle Size:3µm and 7µm, sphericalMobile Phase Limits: pH 2-7, 0-100% organic modifier0 1234567 8 9 Min.Column: Universal Cation HR, 3µm, 7.0 x 53mm(Part No. 23109)Mobile Phase: 3mM Methanesulfonic AcidFlow Rate: 2.5mL/minDetector: ConductivityPACKINGUNIVERSAL CATION COLUMNSPEEKI.D. X LENGTHPART NO.SSPART NO.7µm 4.6mm x 100mm 27106 271003µm HR 4.6 x 100mm – 231007.0 x 53mm – 23109PRICEGUARDS FOR UNIVERSAL CATION COLUMNSDESCRIPTION QTY. PART NO. PRICEGC-2 Universal Cation 3 271010GC-3 Universal Cation HR 3 23110All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler Included.Need an All-Guard holder? Save money with ICAll-Guard Kits on page 316.© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.311

Cations and Organic AcidsI C C O L U M N SHamilton Cation & Organic Acid ColumnsHamilton PRP®-X200 Cation Analysis ColumnsFor separation of the alkali metal and alkaline earthcations in separate runs.Hamilton PRP®-X300 Organic Acid ColumnsFor separation of simple aliphatic carboxylic acids andalcohols. Inorganic anions are not retained.Alkali MetalsEndothall520491461. Lithium2. Sodium3. Ammonium4. Potassium5. Cesium1. Maleic Acid (1160ppm)2. Malonic Acid (2000ppm)3. Succinic Acid (210ppm)4. Fumaric Acid (12.3ppm)5. Endothall (9080ppm) Column: PRP ® -X200, 10µm, 4.1 x 150mm(Part No. 79441)Mobile Phase: 0.004N HNO3 in 30% MeOHFlow Rate: 2mL/minDetector: ConductivityColumn: PRP ® -X300, 7µm, 4.1 x 150mmMobile Phase: 1.5mN Sulfuric Acid: Acetonitrile (92:8)Flow Rate: 0.5mL/minDetector: UV at 210nmHamilton PRP ® -X200 SpecificationsComposition:Sulfonated psDVB copolymerParticle Size:10µm, sphericalExchange Capacity: 35 umeq/gMobile Phase Limits: pH 1-13, 0-100% organic modifierHamilton PRP ® -X300 SpecificationsComposition:Sulfonated psDVB in H+ formParticle Size: 3µm and 7µmMobile Phase Limits: pH 1-13, 0-100% organic modifierHAMILTON PRP ® -X200 COLUMNSPARTICLE SIZE I.D. X LENGTH PART NO. PRICEPRP ® -X200 2.1 x 150mm 793942.1 x 250mm 793474.1 x 150mm 794414.1 x 250mm 79442HAMILTON PRP ® -X300 COLUMNSPARTICLE SIZE I.D. X LENGTH PART NO. PRICEPRP ® -X300, 3um 4.1 x 150mm 79819PRP ® -X300, 7um 4.1 x 150mm 794644.1 x 250mm 794654.6 x 150mm 79485HAMILTON PRP ® -X200 GUARD KITS AND CARTRIDGESDESCRIPTION QTY PART NO. PRICEGuard Column Starter KitPRP®-X200, 1 Holder, 2 CartridgesReplacement Guard Cartridges(Requires Hamilton Guard Holder)PRP ® -X200, 25 x 2.3mmea 794565 79449HAMILTON PRP ® -X300 GUARD KITS AND CARTRIDGESDESCRIPTION QTY PART NO. PRICEGuard Column Starter KitPRP ® -X300, 1 Holder, 2 CartridgesReplacement Guard Cartridges(Requires Hamilton Guard Holder)PRP ® -X300, 25 x 2.3mmea 794605 79453312© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.

I C C O L U M N SOrganic AcidsAlltech Organic Acid ColumnsAlltech OA and IOA• Analyze organic acids and alcohols in a single run• Polymer-based for broad pH stabilityOA: selective for aliphatic and aromatic acidsUse OA-1000 for inorganic anions and most organicacids. Use OA-2000 for low pKa organic acids, lowmolecular weight straight chain acids, and aromaticacids.IOA: separate short chain organic acids from fructoseand glucoseUse IOA-1000 for TCA or Krebs cycle acids,carbohydrates, and alcohols. Use IOA-2000 for fastseparation of acids and some alcohols.Prevail OA• Modified silica for higher flow rates and broader pHstability• Acid-stable for long column lifetimes• Does not require a column heaterPrevail OA (Organic Acid) Columns separate organicacids with high efficiency, sharp peaks, and short runtimes. Use pH adjustments to adjust the selectivity fordi-and tri-carboxylic acids.Wine Analysis by High ResolutionIon ExclusionColumn: IOA-1000, 9µm, 7.8 x 300mm (Part No. 9646)Mobile Phase: 0.005N Sulfuric AcidFlow Rate: 0.3mL/minColumn Temp: 60°CDetector: RI 1. Citric Acid2. Tartaric Acid3. Glucose4. Malic Acid5. Fructose6. Acetic Acid7. Glycerol8. Lactic Acid9. Methanol10. Ethanol5411 Organic Acids Column: Prevail Organic Acid, 5µm, 4.6 x 150mm(Part No. 88640)Mobile Phase: 25mM KH2PO4, pH2.5Flow Rate: 1.5mL/minDetector: UV at 210nm93841. Oxalic Acid 5. Lactic Acid2. Tartaric Acid 6. Acetic Acid3. Formic Acid 7. Diglycollic Acid4. Malic Acid 8. Maleic Acid9. Citric Acid10. Succinic Acid11. Fumaric AcidOA and IOA SpecificationsComposition: Highly sulfonated poly (styrenedivinylbenzene)cation exchangerParticle Size: 6.5µm, 8µm, and 9µmMobile Phase Limits: pH 1-14, isocratic aqueous mobile phasesonly; no organicsPrevail OA SpecificationsComposition: Silica base, with specially modifiedreversed-phase functional groupsParticle Size: 3µm and 5µmMobile Phase Limits: pH 1-9, 0-100% organic modifierALLTECH ORGANIC ACID COLUMNSPACKING I.D. X LENGTH PART NO. PRICEOA-1000, 9µm 6.5 x 300mm 9046OA-2000, 6.5µm 6.5 x 100mm 9048IOA-1000, 9µm 7.8 x 300mm 9646IOA-2000, 8µm 6.5 x 150mm 9648PREVAIL ORGANIC ACID COLUMNSPACKING I.D. X LENGTH PART NO. PRICEPrevail OA, 3µm 4.6 x 100mm 886504.6 x 150mm 88655Prevail OA, 5µm 4.6 x 150mm 886404.6 x 250mm 88645GUARDS FOR OA AND IOA COLUMNSDESCRIPTION QTY. PART NO. PRICEOrganic Acid Guard Cartridge KitIncludes: 1 Holder, 2 Cartridges© Copyright 2004 Alltech Associates, Inc.ea 28883Organic Acid Cartridge, 24 x 4.0mm 2 28884PREVAIL ORGANIC ACID ALL-GUARD CARTRIDGESDESCRIPTION QTY. PART NO. PRICEPrevail OA (holder required) 3 96429All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler included.Contact your Alltech office or distributor for current or local prices.313

Transition MetalsI C C O L U M N SAnion ExclusionAlltech Anion Exclusion Columns• Separate organic acids and weak acid anions• Polymer-based for broad pH stability• Economical choice for small inorganicsAnion Exclusion Columns separate organic acids andweakly ionized anions by an anion exclusion mechanism.Dilute mineral acids are the typical mobile phases forthe separation. Acetonitrile may be used as an organicmodifier to decrease the retention of hydrophobiccompounds.Transition MetalAlltech Transition Metal Columns• Separate divalent transition metals and metal-cyanocomplexes more selectively than a cation exchangecolumnTo separate divalent transition metals, useconductivity detection for concentrations as low as1ppm, or UV detection with post-column reaction forppb-level concentrationsTo separate metal-cyano complexes, use Alltech’s lowUV IPC A (Part No. 185149) as the ion-pair reagent, andmethanol or acetonitrile as the organic modifier.Organic Acid StandardsMetal-Cyano Complexes1303753581. Oxalic Acid2. Maleic Acid3. Malic Acid4. Succinic Acid5. Formic Acid6. Acetic Acid12 3 450 10 20 30 40 Min.1. Fe(CN)6 4-2. Ag(CN)2 -3. Fe(CN)6 3-4. Ni(CN)4 2-5. Au(CN)2 - Column: Anion Exclusion, 10µm, 300 x 7.5mm(Part No. 269062)Mobile Phase: 1.5mM Sulfuric AcidFlow rate: 0.4mL/minDetector: ConductivityAnion Exclusion SpecificationsComposition: Highly sulfonated poly (styrenedivinylbenzene)cation exchangerParticle Size: 10µmMobile Phase Limits:

I C C O L U M N SSurfactantsAlltech Surfactant ColumnsSurfactant/R IC Columns• Separate anionic and cationic surfactants• Separate short and long chain surfactants in onegradient runThe Surfactant/R Column separates anionic andcationic surfactants by ion-pair chromatography alongwith suppressed conductivity detection. This column canalso be used for other reversed-phase applications.Surfactant/C IC Columns• For non-suppressed analyses• Silica-based for symmetrical peak shapesChoose Surfactant C8 for short chain anionicsurfactants such as alkyl sulfates and alkyl sulfonates.Choose Surfactant C18 for long chain or aromaticsurfactants such as xylene sulfonate and dodecylbenzene sulfonate.Short and Long Chain Cationic SurfactantsAlkyl Sulfates1. Tetramethyl Ammonium (20ppm)2. Tetraethyl Ammonium (20ppm)3. Tetrapropyl Ammonium (20ppm)4. Tetrabutyl Ammonium (30ppm)5. Dodecyltrimethyl Ammonium (30ppm)6. Cetyltrimethyl Ammonium (40ppm)7. Myristyltrimethyl Ammonium (50ppm)103171. Octyl Sulfate2. Decyl Sulfate3. Dodecyl Sulfate5998 Column: Surfactant/R Column, 7µm, 4.6 x 150mm(Part No. 25101)Mobile Phase: A: 2mM Nonafluoropentanoic AcidB: 100% AcetonitrileGradient: Time: 0 5 8 20%B: 30 30 35 80Flow rate: 1.0mL/minDetector: Suppressed ConductivityColumn:Mobile Phase:Flow rate:Detector:Surfactant C8, 5µm, 4.6 x 250mm(Part No. 269072)0.02g/L Ammonium Acetate in40% Methanol1.2mL/minConductivitySurfactant/R SpecificationsComposition: Polydivinylbenzene (DVB) based resinParticle Size: 7µmMobile Phase Limits: pH 1-14, 0-100% organic modifierSurfactant/C SpecificationsComposition: Silica-based C8 or C18Particle Size: 5µmMobile Phase Limits: pH 2-7, 0-100% organic modifierPACKINGI.D. X LENGTHSURFACTANT/R COLUMNSPEEKPART NO.SSPART NO.Surfactant/R, 7µm 4.6 x 150mm 25101 25100PRICEPACKINGSURFACTANT/C COLUMNSPEEKI.D. X LENGTHPART NO.SSPART NO.C8, 5µm 4.6 x 250mm 269072 269092C18, 5µm 4.6 x 250mm 269071 269091PRICEGUARDS FOR SURFACTANT/R COLUMNSDESCRIPTION QTY. PART NO. PRICEGRP-1 Revised Phase 3 38124All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler included.GUARDS FOR SURFACTANT/C COLUMNSDESCRIPTION QTY. PART NO. PRICEGRP-2 Revised Phase 3 38129All-Guard Cartridge Holder* ea 80101*Direct-Connect Column Coupler included.Need an All-Guard holder? Save money with ICAll-Guard Kits on page 316.© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.315

Guard CartridgesI C C O L U M N SIC Guard Cartridges• Convenient - change cartridges in seconds withouttools• No connecting tubing required• All-Guard Holders work with other manufacturer’scolumnsInert, metal-free PEEK fluid pathProtect your IC columns from contaminants withAll-Guard Cartridges. The All-Guard System’s uniqueholder is open on the side for fast and easy installationof cartridges.Save money with IC Guard Cartridge Starter Kits,including an All-Guard Holder and three guardcartridges. Connect the All-Guard Holder directly toyour analytical column using Alltech’s patented Direct-Connect Column Coupler, included with each holder.5419Guard Reference TableCOMPANYCOLUMN TYPECARTRIDGETYPEALL-GUARD KIT PART NO.Alltech Allsep , Allsep A-2 GA-1 38109Novosep A-1 GA-1 38109Novosep A-2 GA-1 38109Anion/R, Anion/S GA-1 38109Universal Cation GC-2 27109Universal Cation HR GC-3 23115Anion Exclusion GIE-1 38117Transition Metal C18 GRP-2 38123Surfactant/R GRP-1 38122Surfactant C8, C18 GRP-2 38123Dionex IonPac AS4, AS4A, AS4A-SC, GA-1 38109AS7, AS9, AS10, AS11, AS12,AS14IonPac CS3, CS10, CS11 GC-1 38113IonPac CS12, CS14, CS15 GC-2 27109IonPac NS1 GRP-1 38122IonPac AS1, AS5 GIE-1 38117Waters IC-Pak -A GA-1 38109IC-Pak -M/D GC-2 27109Ion Exclusion GIE-1 38117µBondapak C18 GRP-2 38123Radial-Pak C18 GRP-2 38123Nova-Pak C18 GRP-2 38123Hamilton PRP ® -X100 GA-1 38109PRP ® -X200 GC-1 38113PRP ® -X300 GIE-1 38117PRP ® -1 GRP-1 38122Misc. Sarasep ANIONSEP AN1 GA-1 38109Bio-Rad ® , Aminex ® , HPX-87 GIE-1 38117Interaction ® ION-120 GA-1 38109Interaction ® ION-210 GC-1 38113Interaction ® ORH-801 GIE-1 38117Metrohm Metrosep GA-1 38109Metrohm SUPER SEP GC-2 27109ALL-GUARD KITSDESCRIPTION QTY. PART NO. PRICEAll-Guard Kits (Includes 3 PEEK Cartridges, 1 Holder,and 1 Direct-Connect Column Coupler)GA-1 Anion ea 38109GC-1 Cation ea 38113GC-2 Universal Cation ea 27109GC-3 Universal Cation HR ea 23115GIE-1 Anion Exclusion ea 38117GRP-1 Resin Reversed-Phase ea 38122GRP-2 Silica Reversed-Phase ea 38123Guard Holder (Includes Direct-Connect Column Coupler)All-Guard Cartridge Holder ea 80101Replacement Column Coupler ea 28191REPLACEMENT CARTRIDGESDESCRIPTION QTY. PART NO. PRICEAll-Guard Replacement Cartridges, PEEKGA-1 Anion 3 38108GC-1 Cation 3 38114GC-2 Universal Cation 3 271010GC-3 Universal Cation HR 3 23110GIE-1 Anion Exclusion 3 38118GRP-1 Resin Reversed-Phase 3 38124GRP-2 Silica Reversed-Phase 3 38129316© Copyright 2004 Alltech Associates, Inc.Contact your Alltech office or distributor for current or local prices.