anaerobic bacteria

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CULTIVATIONInoculation of specimen :• Specimens for anaerobic culture must beinoculated onto aerobic medium along with theanaerobic media.• Inoculation of anaerobic liquid media such asthioglycolate medium is also very important forthe possibility of anaerobic incubation systemfails.
CULTIVATION :Anaerobic Media:The media must be,* supplemented with hemin and vit K ,* freshly prepared and properly stored.Solid media ;-Non-selective media• Anaerobic blood agar• Schaedler agarLiquid media ;Thioglycolate mediumChopped meat broth• Brucella agar• Columbia agar-Selective media• Phenyl ethyl alcohol agar• Bacteroides bile esculin agar
Page 3 and 4: Isolation and identification of ana
Page 5 and 6: TRANSPORTATIONSpecimens should be t
Page 7: DIRECT EXAMINATION :Microscopic exa
Page 12 and 13: Examination of growth:Incubation pe
Page 14 and 15: Laboratory practice:On the front be
Page 16 and 17: Pigmented anaerobic bacteria Prevot
Page 18 and 19: Lecithinase (+)
Page 20 and 21: Thioglycolate mediumChopped meat br
Page 22 and 23: Clostridium tetani, gram positive b
Page 24 and 25: Clostridium perfringens, gram posit
Page 26 and 27: Actinomyces meyeriFilamentous gram

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