View - ResearchGate

278 Tikhmyanova et al.Fig. 6. Detailed flow chart for characterization and second confirmation of primarypositives. See text for details.The protocols described next provide two different approaches for analyzingpositives: the first is encouraged. These approaches are summarized in the flowchart in Fig. 6. Both utilize similar methods, but the order with which techniquesare applied differs. The choice between strategies depends on whetherthe individual investigator would rather spend time and money doing bulk PCR