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246 Hust et al.Fig. 1. Schematic description of the selection of antibodies from antibody libraries(panning) by phage display.5. M13K07 Helperphage (Stratagene, Amsterdam, Netherlands).6. 2X TY media: 1.6% (w/v) tryptone, 1% (w/v) yeast extract, and 0.5% (w/v) NaCl,pH 7.0.7. 2X TY-T: 2X TY, containing 50 µg/mL tetracycline.8. Super Optimal Broth (SOB) media: 2% (w/v) tryptone, 0.5% (w/v) yeast extract,and 0.05% (w/v) NaCl, after autoclavation add sterile 1% (v/v) of the 2 M Mgsolution, pH 7.0.9. 2 M Mg solution: 1 M MgCl + 1 M MgSO 4.10. Super Optimal Broth Glucose Ampicilin (SOB-GA) SOB, containing 100 µg/mLampicilin and 100 mM glucose.11. SOB-GA agar plates: SOB-GA + 1.5% (w/v) agar–agar.12. 15-cm Petri dishes.13. 2X TY-GA: 2X TY, containing 100 mM glucose and 100 µg/mL ampicilin.14. Glycerin solution: 87% (v/v).